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» LymeNet Flash » Questions and Discussion » Medical Questions » Culture and identification of Borrelia spirochetes in human vaginal and seminal secre

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Author Topic: Culture and identification of Borrelia spirochetes in human vaginal and seminal secre
KarlaL
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Culture and identification of Borrelia spirochetes in human vaginal and seminal secretions

Marianne J. Middelveen, Jennie Burke, Eva Sapi, Cheryl Bandoski, Katherine R. Filush, Yean Wang, Agustin Franco, Arun Timmaraju, Hilary A. Schlinger, Peter J. Mayne, Raphael B. Stricker

ref status: awaiting peer review

http://f1000r.es/4rm

"We have shown that Borrelia spirochetes are present in semen and vaginal secretions of patients with Lyme disease.

Furthermore, virtually identical strains of Borrelia are present in couples having unprotected sex, suggesting that transmission via intimate contact without a tick vector may occur.

The epidemiology and clinical risk of Borrelia sexual transmission remain to be determined."

"Lyme disease diagnosis is based largely upon serological testing using CDC-sanctioned two-tier surveillance criteria supported by FDA-approved commercial test kits.

While most patients in this study did have positive serological test results for Lyme borreliosis, some were considered serologically negative, and the majority of our study subjects did not meet the positive standard as defined by the CDC surveillance criteria (CDC, 2014a).

We were able to detect Borrelia spirochetes in the blood and/or genital secretions of all patients who were clinically diagnosed with Lyme disease, demonstrating that the CDC surveillance protocol is inadequate diagnostically. . .

In addition, if Borrelia spirochetes were transmitted sexually, then patients with false-negative results may unknowingly spread the infection to sexual partners."

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KarlaL

Posts: 694 | From New Lebanon, NY | Registered: Dec 2010  |  IP: Logged | Report this post to a Moderator
Maia_Azure
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Sooooo...how many of ex-boyfriends do I have to think about having a serious discussion with?

And future boyfriends?

And is that data from recently diagnosed or are some chronic stage Lyme patients in there?

Am I never going to date again? [Mad]

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Sick since 2000
Bulls eye 2005
Dx Babesia, Lyme 2014

Posts: 247 | From New Hampshire | Registered: Aug 2014  |  IP: Logged | Report this post to a Moderator
Lymedin2010
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I loooooooove this!!!!!!!

That is exactly how I was able to diagnose my wife 3 years prior to her showing any symptoms. I simply said to myself, I bet she is eventually going to get sick.

Then as months flew by she had resistant bacterial infections, one month she could not walk due to muscle pulled, swelling and leg infection. She got progressively wiped easily and more tired. And now she has snapping and cracking joints.


She now has many more spirochetes in one drop of blood than 3 years ago.


If I can see many spiros in 1 drop of blood, then a mosquito that feeds on us and then immediately another person, can potentially result in transmission. If a mosquito gets in your house, then it has no choice but to feed on your family, and can lead to transmission.


Then there is possible contact transmission, such as mouth to surface to mouth or eyes. A sneeze and a droplet of saliva on the eye can do it or someone with Lyme crying as the transmission.


Just because you get the infection, does not mean you get Lyme symptoms or get them right away. As typical it can take months to years or a life time.

Posts: 2087 | From NY | Registered: Oct 2011  |  IP: Logged | Report this post to a Moderator
Lymedin2010
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Love, love, love.


I've stressed the importance of microscopy from the beginning and literally saw how easy sexual and contact transmission can occur, based upon the number of spirochetes per drop of blood. Borrelia does not have to thrive in human body fluid, it just needs to survive in cyst or bleb form to transmit. After blood microscopy observations one would have to be an idiot not to draw those conclusions. Blood gets EVERYWHERE in the body!!!!!


"spirochetes were observed in cultures of genital secretions from 11 of 13 subjects diagnosed with Lyme disease, and motile spirochetes were detected in genital culture concentrates from 12 of 13 Lyme disease patients using light and darkfield microscopy."

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t9im
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The powers that be will never let this be "peer review published". It refutes there position on the two tier test.

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Tim

Posts: 1111 | From Glastonbury, CT | Registered: Apr 2010  |  IP: Logged | Report this post to a Moderator
Lymedin2010
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Oh yes, they wouldn't have it. That is why it is published by multiple researchers and in 3 different labs. They knew that they would be refuted and that this would would be a concerted effort.

Time and again the effort to conceal, deny, and deceive is quite evident.

Posts: 2087 | From NY | Registered: Oct 2011  |  IP: Logged | Report this post to a Moderator
KarlaL
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It is possible to get papers published, which demonstrate the inadequacy of the CDC two-tiered tests. It doesn't seen possible to get the CDC to acknowledge their importance and to change their testing recommendations.

Below are two studies which use newly developed DNA sequencing techniques that provide strong evidence supporting persistence of infection after antibiotic treatment, the presence of other disease causing Borrelia species in the U.S, as well as the insensitivity of the CDC endorsed two-tier assay for Lyme disease.

Clark, K.L., Leydet, B., and Hartman, S. (2013). Lyme borreliosis in human patients in Florida and Georgia, USA. Int. J. Med. Sci. 10, 915-931.doi: 10.7150/ijms.6273: http://www.medsci.org/v10p0915.htm

Dr. Kerry Clark's discussion of his research: http://aces.panopto.auburn.edu/Panopto/Pages/Viewer.aspx?id=728531f7-273a-4cb5-970d-9d35c4d9c5cb

Lee, S.H.; Vigliotti, J.S.; Vigliotti, V.S.; Jones, W.; Moorcroft, T.A.; Lantsman, K. DNA Sequencing Diagnosis of Off-Season Spirochetemia with Low Bacterial Density in Borrelia burgdorferi and Borrelia miyamotoi Infections. Int. J. Mol. Sci. 2014, 15, 11364-11386: http://www.mdpi.com/1422-0067/15/7/11364

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KarlaL

Posts: 694 | From New Lebanon, NY | Registered: Dec 2010  |  IP: Logged | Report this post to a Moderator
   

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