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» LymeNet Flash » Questions and Discussion » Medical Questions » China's Wb Criteria for POS...Check this out!!!

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Author Topic: China's Wb Criteria for POS...Check this out!!!
Pinelady
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GO CHINA GO!!!----http://www.ncbi.nlm.nih.gov/pubmed/21112481 2010 Oct Abstract

OBJECTIVE: Western blotting (WB; immunoblotting) is a widely used tool for the serodiagnosis of Lyme borreliosis (LB), but so far, no generally accepted criteria for its performance and interpretation have been established in China. The present study was designed to determine the criteria for standardized Western blot for the predominant species of Borrelia burgdorferi sensu lato in China, in which WB was produced with strain PD(91) as the representative strain attributed to predominant genospecies Borrelia garinii of Borrelia burgdorferi sensu lato.

METHODS: Approximately 13 bands between 14 and 100 kD were differentiated for strain PD(91) by using Gel-Pro analysis software. In a study with 631 serum samples (taken from 127 patients with Lyme borreliosis and 504 controls), all observed bands were documented. To establish criteria for a positive WB result for strain PD(91), receiver operating characteristic (ROC) curves were used.

RESULTS: The following interpretation criteria were recommended:

for IgG, at least one band of P83/100, P58, P39, P30, OspC, P17, P66, and OspA;

for IgM, at least one band of P83/100, P58, OspA, P30, OspC, P17 or P41.

In addition, syphilis, leptospirosis and other related diseases should be excluded when the positive band is P41 in IgM.

For IgG criteria, the sensitivity is 73.2%, the specificity is 99.4% and Youden index is 0.726; for IgM criteria, the sensitivity is 50.6%, the specificity is 93.1% and Youden index is 0.437.

CONCLUSION: Standardization of WB assays is necessary for comparison of results from different laboratories. Moreover, the criteria of other genospecies of Borrelia burgdorferi sensu lato should be determined in the future to complete the criteria of WB for the diagnosis of the Lyme disease in China.

As they look for themselves they have discovered the also need criteria for Pos....Notice sensitivity is still what I consider low while the specificity is extremely high!!!!! Which means we may still need to kill some first to be seen on the testing.....

http://www.ncbi.nlm.nih.gov/pubmed/21106783 2010 Nov 24
Distribution of Borrelia burgdorferi sensu lato in China.
Abstract We
genotyped 102 Borrelia burgdorferi sensu lato strains isolated from
ticks, animals, and patients in 11 provinces in China by PCR restriction
... fragment length polymorphism (PCR-RFLP) amplification of 5S (rrf)-23S
(rrl) ribosomal DNA spacer amplicons and multilocus sequence analysis

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
lou
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It seems that the Chinese actually want to find cases, not ignore them and make sure they don't get treated, like some countries we know of.
Posts: 8430 | From Not available | Registered: Oct 2000  |  IP: Logged | Report this post to a Moderator
Pinelady
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http://www.canlyme.com/wb.html
The CDC criteria for a positive WB are as follows:

* For IgM, 2 of the following three bands: OspC (22-25), 39 and 41.

* For IgG, 5 of the following ten bands: 18, OspC (22-25), 28, 30, 39, 41, 45, 58, 66 and 9...3.

Can we now estimate how many patients have been left to die with their syndrome of unknown origin because of the lies and failures?

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
momlyme
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[shake] Shedding a tear for humanity & praying for humane treatment of people who have TBDs.

--------------------
May health be with you!

Toxic mold was suppressing our immune systems, causing extreme pain, brain fog and magnifying symptoms. Four days after moving out, the healing began.

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lululymemom
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I hope the Chinese will set a precident for the rest of the world... It all has to start somewhere.. [Smile]

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IGM 41 IND, 83-93+ IGG 31 IND,34 IND, 41++, 58+, 83-93 IND

31 Epitope test neg.

Bartonella henselae 1:100

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Pinelady
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USA MUST HAVE MISSED THE MEMO...SOOOO CHINA POSTED IT AGAIN...LOL

http://www.ncbi.nlm.nih.gov/pubmed/23425802

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
ticksickfamily
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Here is a link to the full paper. Note only one band IgM plus one band IgG is needed to confirm Lyme Disease in China and this includes band 41 on IgM. This is for Borrelia afzelii

http://www.besjournal.com/Articles/Archive/2013/No3/201302/t20130220_77498.html

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Razzle
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And with global travel and bird migrations between China and N. America, this strain has to be here in the US as well.

--------------------
-Razzle
Lyme IgM IGeneX Pos. 18+++, 23-25+, 30++, 31+, 34++, 39 IND, 83-93 IND; IgG IGeneX Neg. 30+, 39 IND; Mayo/CDC Pos. IgM 23+, 39+; IgG Mayo/CDC Neg. band 41+; Bart. (clinical dx; Fry Labs neg. for all coinfections), sx >30 yrs.

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Pinelady
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IMO the only diff. between any of the varients is the host and the other infections sharing their genes. The spirochetes are all the same. Its only the other genes they can share that makes the diff.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
Lymetoo
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Wonder how they will TREAT Lyme?

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--Lymetutu--
Opinions, not medical advice!

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Robin123
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Well, even here, Lymetoo, someone stopped a case of Lyme disease by doing PEMF machine treatment (electromagnetic pulse treatment) and Chinese herbs. We have many wonderful Chinese herbalists in town.

Me, I tried Chinese herbs and reacted to the majority of them, so not for me. For others, yes.

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droid1226
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Without an agenda driven IDSA, they may be our best hope.

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http://www.youtube.com/user/droid1226/videos?view=0&flow=grid

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beaches
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Thanks so much for posting this! I am printing the article for a doc or two of mine.
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trimom
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Didn't Dr. H recently go to China consulting with government about TBD?
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Sammi
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Isn't that something? They even consider band 41 on the IgM as a positive.

They do not list bands 23, 31, or 34 which are all Lyme specific.

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Kudzuslipper
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2 years ago, my PCP started treating me with just band 41 because she said "in china they treat with one band." I am very lucky to have her.
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Pinelady
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http://www.besjournal.com/Articles/Archive/2013/No7/201307/t20130729_85328.html

So why did US lie and give us guidelines we could not reach?

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
Annie C
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And people are questioning the Q-RiBb. The CDC accepted it in 2008. I don't see the reasoning of why it's not reliable. When are people and I'm referring to Drs even LLMDs. People have gotten better. Or there wouldn't be the Success topic.

We all want to get better. And NOT just told we are better. Arrrrrgghggggg [group hug] [tsk] [woohoo]

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May God Bless you every day. And Never say never and do not give up no matter what. We need you to help others.

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map1131
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Chinese medicine man pretty smart!!!! I bet they've got the cure before US even thinks that Lyme disease exists or just one pill and it's gone mentality.

Tutu, why'd you have to go and get me ticked off?
[Razz]

Pam

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"Never, never, never, never, never give up" Winston Churchill

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