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» LymeNet Flash » Questions and Discussion » Medical Questions » New Lyme test??

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Author Topic: New Lyme test??
Chipster
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Has anybody seen this report and if so, what do you think?

It is supposed to be for a new DNA sequencing test done by a hospital in Connecticut.

http://wtnh.com/2014/01/15/first-reliable-dna-test-for-lyme-disease/

Chipster

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Tincup
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I did a quickie search and couldn't find much of anything about it, but I didn't search like my life depended on it either. What I did see was almost "evasive", or just totally lacking solid information for some reason.

One good point, it was designed to detect B. Miyamotoi, a strain not normally tested for by commercial labs.

A bad point, quote- "The new test cannot detect older infections; after a month or so the protein it searches for is no longer in a patient's blood."

Sources:

Milford Medical Laboratory in Connecticut is now offering a Sanger sequencing-based test for Borrelia burgdorferi and Borrelia miyamotoi, which cause Lyme disease and Lyme disease-like infection. The test sequences a target segment of the bacterial16S ribosomal RNA gene and is available for $150.
http://www.genomeweb.com/sequencing/new-products-sequenoms-maternit21-plus-milford-medical-labs-lyme-disease-test

In 2010 this was published.

http://www.ctpost.com/local/article/Test-uses-DNA-to-detect-Lyme-disease-434235.php

--------------------
www.TreatTheBite.com
www.DrJonesKids.org
www.MarylandLyme.org
www.LymeDoc.org

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Chipster
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Tincup,

Seems like it doesn't add much, does it?

Chipster

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6t5frlane
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Repost...but worth it. I did not see where it would not help older infections
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Keebler
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-
http://www.anapsid.org/lyme/lymeseroneg.html

Reasons for False Negative (Seronegative) Test Results in Lyme Disease


http://publichealthalert.org/uploads/2013_6.pdf

Public Health Alert (PHA) is a newspaper committed to researching and investigating Lyme Disease and other chronic illnesses

Laboratory Tests and Diagnosis for Lyme Disease and Co-infections - June 2013

- by Armin Schwarzbach, M.D., Ph.D. - Five Pages


http://flash.lymenet.org/scripts/ultimatebb.cgi?ubb=get_topic;f=2;t=015508;p=0

Diagnosing Lyme Disease (&/or whatever else is going on)

Other tick-borne infections and other chronic stealth infections - as well as certain conditions that can hold us back - are discussed here.


http://flash.lymenet.org/scripts/ultimatebb.cgi?ubb=get_topic;f=2;t=013239;p=0

What is a LLMD? LL ND? What is ILADS?

WHY you need an ILADS "educated" or "minded" Lyme Literate doctor (whether MD or ND, or both) - starting with assessment / evaluation for lyme, OTHER tick-borne diseases, and other chronic stealth infections - and all that goes along for the ride.

Medical "models" explained here, as to differences in the ISDA & ILADS models of assessment & treatment - and exactly why it is so very important to know the differences.
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Tincup
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Don't think so Chipster, but there may be (probably is) more info I didn't locate.

To answer 6t5... If you check the second link I posted above you'll see this statement.

"The new test cannot detect older infections; after a month or so the protein it searches for is no longer in a patient's blood."

--------------------
www.TreatTheBite.com
www.DrJonesKids.org
www.MarylandLyme.org
www.LymeDoc.org

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miyamotoi
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This is an important development. PCR with sequencing has a potential to detect and identify the exact species and strain of Borrelia. The current antibody test is very sensitive to Bb strain and almost always fails with other species. There have been at least 4 other species of Borrelia found in the US besides the common burgdorferi species.

B. bisettii

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3067736/

B. miyamotoi

http://www.nejm.org/doi/full/10.1056/NEJMc1215469

B americana and B andersonii


http://www.medsci.org/v10p0915.htm

This is essentially the same test and sequencing used by both Lane and Clark in detecting the new species in human blood.


http://kw.typepad.com/lyme/sample-lyme-report.html


Nested PCR can be up to a thousand times more sensitive. It is the type of PCR used by most University researchers because it can detect down to a low number of spirochetes and by performing it multiple times, the odds of finding a spirochete can be multiplied.

By then sequencing the detected genes, the exact species and strain can be identified from the NCBI and other databases.

So its the first time nested PCR that can catch any species of Borrelia in fairly low quantities with sequencing has been made available outside research labs.

The researcher in this lab has published a number of papers describing their nested PCR procedure and its being done correctly.

You can read about it here:

http://www.mdpi.com/1422-0067/14/6/12853

http://ajcp.ascpjournals.org/content/133/4/569.full


There is no magic in Sanger sequencing as this is the underlying method Frederick Sanger received a Nobel Prize in Chemistry for its invention.

Since the test only costs $150, its possible to double the odds of finding Borrelia by 2X for $300 and quadruple for $600 etc.. The combination of the nested PCR, sequencing and low price do make this an important new test.

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miyamotoi
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Here is a link to the lab describing the nested PCR and sequencing:

http://www.dnalymetest.com/

And the sequencing:

http://www.dnalymetest.com/dnasequencetesting.html

Posts: 51 | From California | Registered: Aug 2013  |  IP: Logged | Report this post to a Moderator
   

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