LymeNet Flash: Berberine and multi-drug resistance

LymeNet on Facebook

LymeNet on Twitter

Follow @LymeDiseaseNet

The Lyme Disease Network receives a commission from Amazon.com for each purchase originating from this site.

When purchasing from Amazon.com, please
click here first.

Thank you.

Dedicated to the Bachmann Family

LymeNet needs your help:
LymeNet 2020 fund drive

The Lyme Disease Network is a non-profit organization funded by individual donations.

»	LymeNet Flash » Questions and Discussion » Medical Questions » Berberine and multi-drug resistance - efflux pumps

UBBFriend: Email this page to someone!

Author

Topic: Berberine and multi-drug resistance - efflux pumps

Marnie
Frequent Contributor (5K+ posts)
Member # 773

posted

Pathogenic bacteria such as salmonella possess RamA, *an activator protein for AcrAB.*

They also have RamR, a transcriptional *repressor* of the gene-encoding RamA protein that

controls the expression of the multidrug efflux system genes.

In antibacterial substance-free conditions,

RamR proteins bind to DNA and

represss the expression of multidrug efflux system genes acrAB's activator RamA.

However, when bacteria are exposed to antibacterial compounds, RamR proteins recognize these compounds,

***reducing suppression of RamA
and thereby promoting multidrug efflux.***

http://www.osaka-u.ac.jp/en/news/ResearchRelease/2013/06/20130626_1

Berberine binds to RamR and upregulates ramA and acrAB

RamR is a transcriptional *repressor* of the gene-encoding RamA protein, which controls the expression of the multidrug efflux system genes acrAB-tolC.

http://www.nature.com/ncomms/2013/130626/ncomms3078/full/ncomms3078.html?WT.ec_id=NCOMMS-20130703

Gram-negative bacteria employ efflux pumps to expel unwanted materials from their cytoplasm.

Cells rely on this process for survival.

The pump is transient and consists of three proteins when it is complexed.

Two inner-membrane proteins make up the translocase complex, which recognizes specific substrates and provides energy for the expulsion step.

When the translocase complex is bound to its export substrate,

it recruits a third, outer membrane protein known as TolC.

The complex is capable of expelling intruders in a single energy-dependent step across both the outer and inner membranes and across the periplasmic space between them.

This is possible because TolC stretches between the two membranes, creating a direct path from the cytoplasm to the extracellular space

http://biology.kenyon.edu/BMB/Jmol2008/KatieKristinaTolC/index.htm

OspA,B and C???

Note...!!!

1. Which form of berberine is most effective...chloride or sulfate?

2.What doses are needed?

3. What timing is needed?

4. What drugs/other supplements should NOT be taken in conjunction with berberine?

5.What drugs/other supplements have a synergy with berberine?

6. Which persons should NOT take berberine (diabetics on insulin come to mind)?

Posts: 9430 | From Sunshine State | Registered: Mar 2001 | IP: Logged |

Keebler
Honored Contributor (25K+ posts)
Member # 12673

posted

-
Marnie,

Thanks so much for this. That last link has some splendid detail on drug resistance and the efflux pump uniqueness of gram-negative bacteria (of which Bb is one).

I could read only a little bit but will come back to this "treasure trove" of information, probably many times before it's all read. Glad you are such a good scout of detail.
-

Posts: 48021 | From Tree House | Registered: Jul 2007 | IP: Logged |

Printer-friendly view of this topic

The Lyme Disease Network is a non-profit organization funded by individual donations. If you would like to support the Network and the LymeNet system of Web services, please send your donations to:

The Lyme Disease Network of New Jersey
907 Pebble Creek Court, Pennington, NJ 08534 USA
http://www.lymenet.org/