LymeNet Flash: Killing of Lymphocytes by Spirochetes

This is topic Killing of Lymphocytes by Spirochetes in forum Medical Questions at LymeNet Flash.

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Posted by treepatrol (Member # 4117) on :

One entry found for lymphocyte.

Main Entry: lym�pho�cyte
Pronunciation: lim(p)-f - s t
Function: noun
: any of the colorless weakly motile cells that originate from stem cells and differentiate in lymphoid tissue (as of the thymus or bone marrow), that are the typical cellular elements of lymph, that include the cellular mediators of immunity, and that constitute 20 to 30 percent of the white blood cells of normal human blood -- see B CELL, T CELL

Figure 1. Micrographs revealing
attachment to and invasion of
lymphocytes by Borrelia burgdorferi.
Cultured SKW 6.4 cells and
primary human peripheral B cells
were coincubated with virulent or
attenuated B. burgdorferi for 1
hour; the mixtures were then prepared
for and examined by light or
electron microscopy. The micrographs
are representative of each
susceptible host cell population. a:
Light microscopy revealed attachment
of spirochetal tips to SKW 6.4
cells; attached spirochetes remained
highly motile yet anchored
to host cells. b: Scanning electron
microscopy revealed that adherent
spirochetes penetrated lymphocytes
through endocytotic pits;
penetrated lymphocytes exhibited
a noticeable loss of filopodia
and other surface projections. c:
Transmission electron microscopy
showed that intracellular spirochetes
were retained within vacuoles;
no fusion of lysosomes to endocytotic
vacuoles was observed.
d: Continued coincubation with
virulent spirochetes resulted in numerous
lymphocytes with disrupted
cell membranes. e: No such
cytopathic changes were observed
with uninfected control lymphocytes.
No such cytopathic changes
were observed with lymphocytes
incubated with Borrelia hermsii or
attenuated B. burgdorferi

Killing of Lymphocytes by Spirochetes

FIG. 1. Attachment of B. burgdorferi to primary murine lymphocytes. Scanning electron microscopy of coincubation mixtures containing B. burgdorferi and immunomagnetic bead-purified lymphocytes (L) showed that spirochetes (S) adhere to immobilized cells but not to the antibody-coated beads (B). Examination of paired stereomicrographs showed that attachment occurred at variable locations along the axis of the spirochete. However, adherence was observed most frequently in association with the terminal ends of filopodia extending from the surface of immobilized lymphocytes. Scale bar, 0.5 �m.

Link PDF

Also

Link Intracellularity

AHMF Data Base

Intracellular localization

[This message has been edited by treepatrol (edited 24 February 2005).]

[ 19. May 2006, 02:06 PM: Message edited by: treepatrol ]

Posted by sarahjane (Member # 6517) on :

Hi,

Thanks for this article!

Especially relevant to me today...just this morning I was searching for answers to why I am so sore on the right side under my arm- and trying to educate myself on the effect of Lyme on the lymphatic system.

Since being infected with Lyme in October and starting treatment just over a month later( 400 mg of doxy/day with various supplements)- I have had times where I am sore under the arms and in the groin.

I had a bit of a scare with a breast lump which after ultrasound and mammogram looks to be a cyst- perhaps also related to the lymphs?

I have read that Bartonella can affect the Lymph more than Lyme- any ideas as to why?

Perhaps I only have Bartonella? This whole tick disease journey began with a "nip" from a tick and the only skin rash has been one round red bump on the same finger that got the nip....( which a Park Ave dermatoligist told me was perfectly normal-ARGH!-)

it is still there and red today actually- same side as my sore lymphs...

So many mysteries with Lyme disease-
Why does it seem to affect one side of the body at a time for instance....?

Thanks for insight and ongoing wisdom-

Sarah

Posted by treepatrol (Member # 4117) on :

Your welcome

Posted by ivebeentricked (Member # 6944) on :

could this cause my lymphocyte count to be low??? I have been trying to figure out what is wrong with me for the past 4 years and I recently looked through my medical records and found that I had high neutrophils, high eosinophils, and low lymphocytes on a number of occasions...I have also tested positive for lyme but the testing method I guess was questionable. So anyway, could this be a reason why my lymphocytes have been low, in some cases when I was most sick they were way low.

thanks

Posted by treepatrol (Member # 4117) on :

quote:
Originally posted by ivebeentricked:
could this cause my lymphocyte count to be low??? I have been trying to figure out what is wrong with me for the past 4 years and I recently looked through my medical records and found that I had high neutrophils, high eosinophils, and low lymphocytes on a number of occasions...I have also tested positive for lyme but the testing method I guess was questionable. So anyway, could this be a reason why my lymphocytes have been low, in some cases when I was most sick they were way low.
thanks

Yes from what I have been reading yes

Posted by kare (Member # 5041) on :

My spinal tap results read "very rare lymphocytes" which I think means very low.

Posted by hiker53 (Member # 6046) on :

Kare,

Very rare lymphocytes in a spinal tap is a good thing. If you have white cells in your spinal fluid that means you have an infection, such as meningitis there.

Posted by Mo (Member # 2863) on :

Hey Tree,

Thanks for this feature film
(remember Saturday afternoon "Creature Feature")

Great info.

How many Lyme patients here have low Lymphocytes?

Would be interesting to know.

[This message has been edited by Mo (edited 24 February 2005).]

Posted by Tincup (Member # 5829) on :

Thanks tree...

Good info!

I'm low... have been for a long time.

Do you think that might mean I have Lyme disease?

Or am I just plain old NUTS?

------------------
If you get the choice to sit it out or dance...

Posted by treepatrol (Member # 4117) on :

quote:
Originally posted by Tincup:
Thanks tree...
Good info!
I'm low... have been for a long time.
Do you think that might mean I have Lyme disease?

Or am I just plain old NUTS?

TC NUTS

And depending on the infections state probaly everyone has had low lymphocytes MO

Posted by kare (Member # 5041) on :

Hiker53:

The funny thing is that the Neuro and ID Drs did not mention the rare lymphochtes.
They do not want to hear about LD. Is this an indicator of LD in the nervous sys?
I definately do have big issues w/ my nervous sys.

Posted by Cher (Member # 7108) on :

I've been low too. But not dx with Lyme, yet.

Posted by janet thomas (Member # 7122) on :

Tin cup- You never fail to crack me up- Thanks Janet

Posted by treepatrol (Member # 4117) on :

up for fix

Posted by ChrisBtheLymie (Member # 8916) on :

My blood tests which I had done on Monday have shown low Lymphocytes.

Posted by Cobweb (Member # 10053) on :

That's discouraging- I can't even understand picture threads even ! [Razz]

Posted by 5dana8 (Member # 7935) on :

Awesome good photo's!! Thanks Tree for posting.

Can't understand why the mdeical community can't look at these phots's & see why BB is so a crafty devil. Do they not believe the photos?

How can they dispute good hard core magnification scienticfic photo's?

Here's a video-look under "spiroketes-sub coil structure
and under borrelia-boaring into human skin cells:

http://www.canlyme.com

Thanks again [Smile]

Dana