LymeNet Flash: Want to see Borrelia Burgdorferi swim, form Cyst an more.

This is topic Want to see Borrelia Burgdorferi swim, form Cyst an more. in forum Medical Questions at LymeNet Flash.

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Posted by lymebug (Member # 7270) on :

Hey everyone,

I researched/studied BB back in 1999. The last 6 years I've worked in Corporate Wasteland. I finally started to put together a website where I could post some video microscopy that I shot back then. It was one of the most interesting times of my life. After the opportunity ended I was never able to find a job in the Chicagoland area studying the bug.

Videos demonstrate.

-Normal swimming motion
-Cyst formation
-Long form BB
-Mass forms of BB
-BB Growth from long thin non-spiral protrusions.

Feel free to reference this site.

This videos were made with the ATCC Strain Borrelia Burgdorferi 31. http://www.atcc.org/catalog/numSearch/numResults.cfm?atccNum=35210

Click here: http://www.freewebs.com/lymebug/

Please allow a long time for the videos to load. You probable will have to have High Speed internet and it will still take 5 minutes or so. Go get a coffee!! Its worth it.

If you have any contacts for me in the Chicago area that would be cool.

------------------
Later
Stan

Posted by pippy (Member # 6005) on :

Thanks Stan!
We will enjoy these! Are you a microbiologist looking to study BB?
There is a contact of a lab in CA. They may need to expand at the rate Lyme is infecting people nationwide now.

Posted by lymebug (Member # 7270) on :

Yes

Microbiologist.

------------------
Later
Stan

Posted by lou (Member # 81) on :

Drat. Got dial up. Will have to go looking for a machine on broadband.

Posted by Jellybelly (Member # 7142) on :

Stan

I get there and it just sits there. I have high speed. Is there something I need to do? I really want to see them swim.

Posted by treepatrol (Member # 4117) on :

I went to first link talking about strains.

second link is just pics no movies.

Posted by lymebug (Member # 7270) on :

Click on the this link:http://www.freewebs.com/lymebug/

Click on Movies or Movies II

You will see Screen with Windows Media LOGO on it.

After a few minutes (5 to 15 min) the LOGO goes away.

Movie is loaded when you see the bug.

Click Play.

You can also click on the scroll bar and fast forward and reverse the action.

Stan

Posted by lymebug (Member # 7270) on :

Better yet direct. Link.
http://www.freewebs.com/lymebug/movies.htm http://www.freewebs.com/lymebug/movies2.htm

Stan

Posted by treepatrol (Member # 4117) on :

Your link produces this no windows logo
http://www.freewebs.com/lymebug/

CLICK ON MOVIES OR MOVIEII

AND GET THIS

MOVIES Please!

Home / Movies / Movies II / Pics / Story / Contact
All movies are Bb31 in BSKII agar. Live slide prep on Darkfield Microscope at 1000X. Use the scroll bar on the movie viewer to toggle back and forth. This will help to see the details of the moment.

Movies will take a few minutes to load with high speed. Longer with dial-up. Go get a coffee.

Subject: Normal Bug Life
Title: Swimmer
Normal textbook morphology. Six spirals long. Motal. Actual speed not sped up. This boy moves. Nonviscous fluid doesn't allow the coils to really grab anything, but the bug does manage to cross the field of view.

A recurring theme is the very little jiggly guys that move around in all of these Bb31 monocultures. I know, I know. "Brownian motion". That explains the random movements but immunoflurecent staining shows objects this size that stain positive. One shows up in the last few seconds of this movie.

Subjects: Morphology and "Cysts"
Title: "Death or Dormant"
Two large "double spirochetes" Bb31 in BSKII agar with penicillin solution added to live slide prep.

Upper left bugs.
The upper "double spirochete" has a few smaller ones near it. You will see three or more after you watch it a few times.

Lower right bugs.
A perfect double spirochete. Double only forms one "cyst". Not sure why, maybe because they are still attached. If separated I think they would form two. Use the slider on the movie projector to go frame by frame to see the spiro uncoil to one long straight form then "cyst" or lyse. Depending on which side of the lyme controversy you stand on. Dead bug or Dormant bug.

Subject: Growth and other "Cysts"
Title: Inside the "Cyst"
A few visible spirochetal "eggs" inside a "cyst"? These cysts are always connected to or form directly from an adult spirochete. The thin young spirochete has either grown out of the "cyst" or made the "cyst". Never the less internal structures are visible.

And I am on T1 line very fast no movie

[This message has been edited by treepatrol (edited 06 May 2005).]

Posted by Areneli (Member # 6740) on :

[This message has been edited by Areneli (edited 06 May 2005).]

Posted by lymebug (Member # 7270) on :

Sorry then.

Not sure what the problem is. When I click it the the first movie shows up right under the

Get a coffee part.

I look into it.

Does anybody see the Window media screen?

------------------
Later
Stan

Posted by treepatrol (Member # 4117) on :

nope

Posted by Areneli (Member # 6740) on :

I see medial player in IE, but nothing playes even after extensive wait.

Other browsers such as Opera or Firefox don't see anything (no media player)

Posted by lymebug (Member # 7270) on :

This the first website I've ever put together. I'm not an expert. All I can do is make it and look at it. I don't know exactly how to make sure it works on all computers.

I had a neighbor open the link and it worked and played in about 30 sec.

Maybe is windows media updates or something.

Sorry for the inconvience.

And I not advertising anything I'm trying to help.

------------------
Later
Stan

Posted by robi (Member # 5547) on :

Wuld love to see these. I have dsl but can't seem to see them.

robi

Posted by lou (Member # 81) on :

Keep trying, Stan. You've got folks who are REALLY interested in this. Thanks.

Posted by robi (Member # 5547) on :

Thanks for posting this Stan......... and helping out the cause ..... ignore the folks with negative comments ....

robi

Posted by Jellybelly (Member # 7142) on :

Thanks Stan. I will let it sit for longer. When yours first loads, do you get the little cursor flashing or an hour glass like something is happening?. I do get the media windows, with buttons to push and play, volume control, but no movies. My cursor just sits there like it is done.

I'll keep trying, cuz I do for some warped reason want to know what the little body snatchers swimming around inside me look like. Motion pictures are far better then snap shots in this case.

Posted by Jellybelly (Member # 7142) on :

Oh my gosh Stan! I just read the little story. Are those YOUR bugs???? They came from a cyst and you filmed them? Wow, pretty amazing. You actually got to watch them!

Posted by fiddler (Member # 3993) on :

Hi all- wanted to bring this back up- looks like many of you weren't able to access videos. I was able to on only a DIAL UP connection & they loaded IN LESS THAN 3 MINUTES. But, you must have the correct interface loaded onto your computer. Hit the link listed below.

THEY ARE GREAT. I have never seen anything like them out there- only pic. Try it for your self.

Click here: http://www.freewebs.com/lymebug/

-Sarah

------------------

Posted by lou (Member # 81) on :

Fiddler,what is the correct interface? When I click install plug-ins, I get a message that none are found. This is mozzilla searching for plug ins. Would sure like to see these movies.

Posted by fiddler (Member # 3993) on :

You need Windows Media Player- that's what it told me anyway when I clicked on movies. In the movie screen it was black except for the words "Windows Media Player", I waited about 3 min., at which point the movie screens went totally black. Then I pressed "Play" & voila- there the little b******* were swimming around. I esp. liked the one which shows how quickly the cypt formed. Try it again- it is so interesting.

Posted by lou (Member # 81) on :

Guess it is time to give up. I have windows media player but it doesn't come up when I click on movies, just get all these empty boxes with button to download plug ins, which doesn't. When I go to windows media player directly and click on enter URL in file menu, it gives me an error message. Says it can't read the file.

Really, computers can be maddening.

[This message has been edited by lou (edited 10 May 2005).]

Posted by lymebug (Member # 7270) on :

Very sorry for the difficulty. I emailing a web designer today to get suggestions on how upload the movies so all can see them. I'll repost when get it figured. I'm on a mission now.

Stan

One thing to try.

I used this codec to compress file.
See if downloading it helps.

Indeo Video 5

Google:
Indeo Video 5 or try link below. http://freepctech.com/pc/002/files011.shtml

Indeo Video Codecs

2mg
Contains the Indeo IR3*, IV4*, and IV5* codecs. Recently the Ligos Corporation took over Indeo video development from Intel Architecture Labs. They have updated Indeo Video to Indeo Video 5.11 and will continue to develop the technology in the future. Please visit the Ligos Corporations website for more information and support.

Posted by Areneli (Member # 6740) on :

I was able to download one of your movies and it works perfectly.

Can you make the others also available for download?

Posted by no2lyme (Member # 6978) on :

Yuck! Somehow seeing them in action makes me skin crawl, literally. Very fascinating and am glad that it was posted. No wonder it takes so long to get rid of them.

Posted by Sam60 (Member # 7311) on :

That was so cool and at the same time really creepy. When I get the bullseye rashes thats what it feels like is going on under my skin and now I'm going to think of that everytime it itches. Thanks for letting us view that.