Microbes & Infection 2006 Sep 22; [Epub ahead of print]
Invasion of human neuronal and glial cells by an infectious strain of Borrelia burgdorferi.
* Livengood JA, * Gilmore RD Jr.
Centers for Disease Control and Prevention, Division of Vector-borne Infectious Diseases, 3150 Rampart Road, CSU Foothills Campus, Fort Collins, CO 80522, USA.
Human infection by Borrelia burgdorferi, the etiological agent for Lyme disease, can result in serious acute and late-term disorders including neuroborreliosis, a degenerative condition of the peripheral and central nervous systems.
To examine the mechanisms involved in the cellular pathogenesis of neuroborreliosis, we investigated the ability of B. burgdorferi to attach to and/or invade a panel of human neuroglial and cortical neuronal cells.
In all neural cells tested, we observed B. burgdorferi in association with the cell by confocal microscopy.
Further analysis by differential immunofluorescent staining of external and internal organisms, and a gentamicin protection assay demonstrated an intracellular localization of B. burgdorferi.
A non-infectious strain of B. burgdorferi was attenuated in its ability to associate with these neural cells, suggesting that a specific borrelial factor related to cellular infectivity was responsible for the association.
Cytopathic effects were not observed following infection of these cell lines with B. burgdorferi, and internalized spirochetes were found to be viable.
Invasion of neural cells by B. burgdorferi provides a putative mechanism for the organism to avoid the host's immune response while potentially causing functional damage to neural cells during infection of the CNS.
PMID: 17045505
Posted by Lymetoo (Member # 743) on :
ugh
Posted by LYMESCIENCE (Member # 9259) on :
great post! This corresponds very well with biology as it exists.
Posted by trails (Member # 1620) on :
then why are they sticking to their stupid BLOOD test?
Posted by treepatrol (Member # 4117) on :
duh
Posted by ldfighter (Member # 9405) on :
Yes, and since cephalosporins are not intracellular, how can they maintain 2-4 wks of rocephin cures CNS Lyme???
This CDC study directly contradicts the new IDSA guidelines, which state:
"Lyme disease lacks characteristics of other infections that justify longer treatment courses, such as... infections caused by an intracellular pathogen..."
Of course there's already plenty of evidence that it's intracellular, but this is the first I've seen from the CDC & I think the first that shows Bb specifically invades (doesn't just damage) neural cells.
Posted by Beverly (Member # 1271) on :
ugh and duh
Posted by TerryK (Member # 8552) on :
Great find!
The IDSA states that lyme lacks characteristics of other infections that justify a longer treatment course, such as infections caused by an intracellular pathogen.
Do a search for "intracellular", without the quotes.
They also say that in-vitro, cysts have not shown clinical significance. Terry
Posted by lou (Member # 81) on :
Guess it turns out that not everyone associated with CDC's infectious diseases division is a liar. Now if only the bosses will listen and change their approach.
Thanks for posting this, fighter. An important article!
[ 19. October 2006, 04:55 PM: Message edited by: lou ]
Posted by TerryK (Member # 8552) on :
ldfighter, weird, your post was not shown when I posted mine. Was mine there when you posted yours about the IDSA guidelines?? Terry
Posted by ldfighter (Member # 9405) on :
Nope Terry, I didn't see your post until after I'd already posted, guess we were just in sync. Posted by Robin123 (Member # 9197) on :
So if this is a recent CDC study, what's going on with this organization?! Sounds like one hand doesn't know what the other one's doing, so to speak.
ldfighter, could you by any chance provide the link for this article? In this way, we could send it around easier, possibly even do some forwarding. It directly contradicts the new guidelines. Yay.
Posted by Areneli (Member # 6740) on :
I am sure that the article will be listed by IDSA as 'not to read'
Posted by ldfighter (Member # 9405) on :
The journal is Microbes and Infection, if anyone's able to get the full text.
Posted by lymie tony z (Member # 5130) on :
I don't quite understand...
I've known that BB infects brain and cns cells for years...
Is it just "new" because now the CDC has figured it out!???
Or is it "new" because they admit it!
zman
Posted by Dave6002 (Member # 9064) on :
Have scaned the full text artical: they have done a great job showing that infectious Bb did attach to the neuronal cells and invade neuronal cells by beautiful confocal microscopy pictures.
In contrast, non infectious Bbs cannot attach and invade neuronal cells.
They are going to find out what genes are responsible for the infection (attachment and invasion).
Definitely they are on the right track.
However, this just in vitro experiments, and we know that there could be huge differences between in vitro and in vivo(the reality).
By the way they are scientists not policy maker, that's why they just care about the facts not politics.
There will be more decent papers coming out on Lyme disease.
Dave
Posted by treepatrol (Member # 4117) on :
You guys need to jump on this sounds like this reporter goes after liars and gets prosecutions!!
![[Smile]](smile.gif)