5 September 2004, Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one ``Spironema culicis'' strain recovered from mosquito Culex pipiens and seven spirochetal strains (not identified yet) isolated from mosquitoes and blackflies. ----------------------------------------- 2010 http://www.bioone.org/doi/abs/10.3376/038.035.0123?journalCode=jvec Seven spirochetal strains have been successfully recovered from mosquitoes and black flies: BR149 (Culex pipiens), BR151 (Cx. pipiens), BR173 (Cx. pipiens), BR177 (Cx. pipiens), BR193 (Aedes cinereus), BR208 (Cx. pipiens), and BR231 (Simulium noelleri). The strains have been adapted to laboratory conditions (BSK-H Complete medium). Their preliminary determination based on 16S rRNA gene sequencing has shown that they differ from the Lyme disease spirochete Borrelia burgdorferi sensu lato
as well as other members of the Order Spirochaetales indicating novel bacterial species in the Family Spirochaetaceae. ========================== Could it be these new findings are what some are describing as TBEV causing organisms and they just are a little behind in the science? History in the Making. ------------------------- http://www.ingentaconnect.com/content/bsc/emi/2010/00000012/00000001/art00013 January 2010 Japan The members of the genus Borrelia are transmitted by arthropods and known to be infectious to vertebrates. Here we found isolates and DNAs belonging to the Borrelia turcica and unknown Borrelia species from imported reptiles and their ectoparasites... See More.
The Borrelia strains were isolated from blood and multiple organs of exotic tortoises, and were experimentally infectious to captive-bred tortoises. These findings suggest that these tortoises may be a candidate as the reservoir host of the Borrelia species.
In this study, the Borrelia strains were also isolated from and/or detected in hard-bodied ticks, Amblyomma ticks and Hyalomma ticks. In some of these ticks, immunofluorescence imaging analysis revealed that the Borrelia had also invaded into the tick salivary glands.
Accordingly, these ticks were expected to be a potential vector of the Borrelia species.
Sequencing analyses of both housekeeping genes (flaB gene, gyrB gene and 16S rDNA gene) and 23S rRNA gene-16S rRNA gene intergenic spacer region revealed that these Borrelia strains formed a monophyletic group that was independent from two other Borrelia groups, Lyme disease Borrelia and relapsing fever Borrelia.
From these results, the novel group of Borrelia comprises the third major group of arthropod-transmitted borreliae identified to date.
[ 07-14-2010, 01:38 PM: Message edited by: Pinelady ]
Posted by Pinelady (Member # 18524) on :
Borrelia have a crazy way of being undetected and hidden in the bodies own cytoplasmic membranes as well as biofilms.
Could it be that since the vectors in question have differing membranes they are taking on that genetic cytoplasmic membranes
and that is the only difference, as they are a completely different vector from the usual mammalian hosts,
they simply don't know yet how they behave in others---yet? These and many other questions need answered.... http://www.ncbi.nlm.nih.gov/pubmed/20603210 Pin the tail on the donkey.