This is topic Those with Brucella, did you have a second test done to confirm? in forum Medical Questions at LymeNet Flash.


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Posted by Sammi (Member # 110) on :
 
If you tested positive for Brucella, was a second test done to confirm it? If so, did you test positive on that also?

I tested positive on the Brucella ELISA IgG. My result was 2.81 and anything above 1.11 is positive. I am having a second test done. I believe it is called a tube agglutination test.

Does anyone know how reliable the tube agglutination test is?
 
Posted by aperture (Member # 34822) on :
 
My IgG was 2.47 and IgM was 2.15. My Agglutination test was negative. My LLMD and ID Dr both agree that, with levels this high, I have Brucella.
 
Posted by Sammi (Member # 110) on :
 
aperture, thanks for your reply.

I was told that the Agglutination test is usually negative. I don't know why it is required. I believe my doctor will likely treat me also because of the initial result.

Are you currently treating it? Are you taking Doxy and Rifampin? If so, do you notice any improvment?

It looks like many of the symptoms of Brucella are also common to Babs. Once again, it is hard to tell which symptoms are from which disease!
 
Posted by jlcd1 (Member # 18138) on :
 
I can tell you my results in 2 weeks.

I did about 6 months of rifampin and mino and then added zith. After 2 months on that I felt awesome!!
 
Posted by BoxerMom (Member # 25251) on :
 
I have not tested. I've been on Rifampin, Doxy, Zith since December. Doing well, but now Babs is flaring.

Yes, the symptoms are similar.
 
Posted by aperture (Member # 34822) on :
 
Sammi,

I was on Doxy and Rifampin for the past 2 months. I am currently taking a break because I was herxing constantly. I don't notice any difference...yet.

BTW, I saw my ID Dr today and he feels that, since I have not responded "to the recommended 6wks of Doxy/Rifampin", then he doubts I ever had Brucella and wants me to now see a Rheum. Whatever?!?! So maddening! At least my LLMD agrees with longer term treatment (heck, even the Dept of Health agrees that Brucella can be chronic).

The symptoms are very similar to Babs. I was also on Plaquenil and Artemisinin SOD. But I am on a temporary break from those and focusing on detox and nutritional supplements til I see my LLMD on Thurs.

I get re-tested in a couple of weeks to see if my IgG and IgM levels have responded to treatment.

Good luck!
 
Posted by nonna05 (Member # 33557) on :
 
Same go around for me....Was tested twice with pulm. doc...he said positive/no doubt !!!

Sent to ID and they ran different test and didn't come up with that or anything really and sent to RA or home/

Do something that makes you happy and get on with life..

Easy to say when you don't have to hold off going to the bathroom 2 hours cause you're so sick ,tired, in pain and have so many weird feelings, tingles etc. , etc.

First doc just kept me on Zith and/or Doxy as much as could . Changed insurance lost him and just got the LD diagnosed this AUG.

I had asked the ID Doc's to check for any spirochete illness, malaria..whatever.

I don't think they did ,cause I can't get them to answer that directly at...Now won't even

return call.


That all started over 5 years ago..OH They did do something for me...STEROID SHOTS 6 in less than 5 months,2010..

That took this illness to a whole new horrible level...

Frustrated..today..had some symptoms a bit better. Tried to ignore the feeling they were showing up again ,but after several days guess it's real..
Now showing up [Frown]
 
Posted by nonna05 (Member # 33557) on :
 
anybody else with a Brucellosis tie-in to being sick?//
 
Posted by timaca (Member # 6911) on :
 
I can't speak on Brucella from personal experience, but I can speak from other pathogens, eg: Parvo, CMV, etc. Those results are in the 5 to 7 range for me (reference range for Parvo <.9; CMV <.8) and my doctors are not concerned. This just shows prior exposure. The tests have been repeated over the years and they don't move much which also indicates prior exposure.

So I would think that values in the 2s do not indicate a problem. If those values go up significantly then it warrants paying attention to. Or if you had values in the 15s or higher I'd be talking to a doctor about it.

Brucella can have a false positive IgM. See: http://www.medscape.com/viewarticle/576150_2
If one has a true positive IgM then the IgG would go up with time. (At least that is my understanding)

It will be interesting to see Aperature what your tests show now. What did the ID doctor think about your HHV-6 count? That is the one I would pursue.

Nonna~ call the ID office and ask for copies of your test results. Those are your results and you are entitled to have a copy if you want them.

A steroid shot to my knee set off my illness so I can totally relate to your problems. My ID doctor has seen that in other patients as well.

You might want to look this website over and get tested for some viruses if antibiotics are not helping you: http://chronicfatigue.stanford.edu/

Best, Timaca
 
Posted by Sammi (Member # 110) on :
 
apeture, I wonder if treating Brucella can make symptoms worse for some time like Lyme and company.

I am not surprised about what the ID believes. So your test was positive because of what??!! I have also read that Brucella can be chronic.

I had the tube agglutination test done today, but it sounds like it is rarely positive. Let us know how your follow-up tests are.

I hope your appointment went well today.
 
Posted by aperture (Member # 34822) on :
 
Sammi: My LLMD does believe that Brucella can be chronic. Funny, at the time I was diagnosed, my ID Dr thought so too. Now he thinks I never had it?? This is the same crazy-making, with ID Dr's, that surrounds the Lyme issue.

Be sure to let us know what the results of the agglutination test are. I am having my follow-up Brucella labs done today.

Timaca: my LLMD appt was wonderful! I am so grateful for him/her. He/she is willing to look at anti-virals; specifically the Stanford protocol.

I am going to reassess after I heal from a small oral surgery next week.
 
Posted by timaca (Member # 6911) on :
 
aperture~ Glad to hear that your LLMD appt was good! Keep me posted on lab tests and treatments. Thank God for doctors who are willing to learn and help us!!

Best, Timaca
 
Posted by GlitchyMomma (Member # 35826) on :
 
Hi everyone. I have been analyzing my bloodwork for the last 10 years, and in addition to being CDC positive for lyme, I think I may have brucellosis.

I also have had a persistent back ache around my upper spine for 9+ years, and that really makes me think Brucella.

Also, in 1996 I visited Quito, Ecuador for a week with my "then" boyfriend (now ex-husband). I'm sure that the soft cheeses we ate were likely unpasteurized.

Anyway, I'm trying to figure out what testing I can order from LabCorp. I just left my LLMD last week, so trying to figure out what other doc I can convince to run the test.

I read the Rose Bengal Test was very accurate, along with an additional test for IgG and IgM but I don't know if those can be done with LabCorp or not.

Thanks for any help,
Glitchy
 
Posted by jlcd1 (Member # 18138) on :
 
My results say my brucella abortus tube agglutination titer is 80. I don't know what it was a yr ago, but tested positive then too. Not sure about these other tests, sorry.

I had mine done at a local hospital.

I also have horrible pain in my upper back. That's where the worst of my pain is.
 
Posted by GlitchyMomma (Member # 35826) on :
 
Crazy! Over 9 years and I feel so close to figuring out the root cause! I will feel so vindicated!

At labcorp now, waiting on my LLMD to call me back and order the testing.
 
Posted by Larae30 (Member # 35220) on :
 
I feel like I should be tested for brucella and fry bug...?
 
Posted by Pinelady (Member # 18524) on :
 
http://www.ncbi.nlm.nih.gov/pubmed/21668364 The fragments encoding potential B-cell epitopes were cloned and overexpressed in an E. coli system.

Japan now tells us they see the same in Pam2cys/mycoplasma.
http://www.ncbi.nlm.nih.gov/pubmed/21172450

The results infer that MALP-2 is applicable to a cytokine inducer but not to an adjuvant for antitumor NK immunotherapy.

It is done..
http://www.newswise.com/articles/new-disease-modeling-grants-target-dengue-tb-other-infections

They have no idea how many of what is hiding in the stealth proteins. Why? Because they will not culture...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025832/ Cattle tick assessment.

See what they see in the different morphological forms of vector----what is the difference? The blood they feed on. IMO it does not matter what the tests say when we know they can hide in synergy of the DNA. Treat everything.
 
Posted by GlitchyMomma (Member # 35826) on :
 
Sorry Pine, but I'm not clear of how your post relates to brucella?
 
Posted by Pinelady (Member # 18524) on :
 
They have no idea how many of what is hiding in the stealth proteins. Why? Because they will not culture...
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3025832/ Cattle tick assessment.

The results showed that the PCRs targeting the
OspA gene,
fla gene,
rrs gene, and
P66 gene detected
37 (6.8%),
74 (13.6%),
16 (2.9%), and
14 (2.6%) tick samples,
respectively. The PCR targeting the fla gene was the most sensitive method for the detection of B. burgdorferi s. l.
http://www.ncbi.nlm.nih.gov/pubmed/22494560


See what they see in the different morphological forms of the tick vector----what is the difference? The blood they feed on. IMO it does not matter what the tests say when we know they can hide in synergy of the DNA. Treat everything.
 
Posted by Pinelady (Member # 18524) on :
 
Besides surviving in a host cell, Coxiella burnetti can adapt to a shared intracellular niche with another parasite without inhibition of development for either.
According to research accomplished by Andreoli and colleagues, Vero cells that were previously inhabited by Coxiella burnetti were co-infected with another type of parasite such as Trypanosoma cruzi.

Parasitophorous vacuoles (PV) of T. cruzi trypomastigotes and bacterial vacuoles fused together upon co-infection.

T. cruzi was able to proceed with differentiation into amastigotes and division in Coxiella burnetti vacuoles. The co-existence of both parasites depends on the ability of Coxiella burnetti to provide sufficient amount of nutrients for T. cruzi.
http://microbewiki.kenyon.edu/index.php/Coxiella_burnetti
----------------------------
http://www.biomedcentral.com/1471-2180/11/6
That's why they too can be sero negative.

http://www.biomedcentral.com/1471-2180/11/6/figure/F2
The infections don't just disappear from egg to adult.
 
Posted by Pinelady (Member # 18524) on :
 
Borrelia is all of them and all of them are Borrelia

New detection methods have found it in at least 80% of the pop.s Because it hides in the DNA as shown above.

http://www.youtube.com/watch?feature=player_embedded&v=yOno_2m_8LY

We the people should not be FORCED to wait until we are crippled with Lyme to treat the masses for the truth with the proper treatment=====Antibiotics and Stem Cells.

But the criminals who got us in this predicament of Autism now being diagnosed at 1 in 29 now trial Chemo/Rituxamab in all the syndromes to once again fill their pockets---not make you well. Because MS trials have already shown the disaster it to be.
 
Posted by Pinelady (Member # 18524) on :
 
If you think they don't care to kill us now----don't think they will care when the treatment means antibiotics and stem cells or
chemo/rituxamab...

Because they don't.

Its going to come down to how much you are worth them them to let you live with how much money they can make as a result of what they decide...

If we do not fight for the people now----well they now rehash birth control and abortion to make sure you don't get that chance.
 


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