Bpur, the Lyme disease spirochete's PUR-domain protein: identification as a transcriptional modulator and characterization of nucleic acid interactions.
Jutras B, Chenail AM, Carroll DW, Miller MC, Zhu H, Bowman A, Stevenson B. Source
University of Kentucky, United States; Abstract
The PUR-domain is a nucleic acid-binding motif
found in critical regulatory proteins of higher eukaryotes,
and in certain species of bacteria.
During investigations into mechanisms by which the Lyme disease spirochete controls synthesis of its Erp surface proteins,
it was discovered that the borrelial PUR-domain protein,
Bpur, binds with high affinity
to double-stranded DNA
adjacent to the erp transcriptional promoter.
Bpur was found to enhance the effects of the erp repressor protein, BpaB.
Bpur also bound single stranded DNA and RNA,
with relative affinities RNA > double stranded DNA > single stranded DNA.
Rational site-directed mutagenesis of Bpur identified amino acid residues
and domains critical for interactions with nucleic acids,
and revealed that the PUR-domain has a distinct mechanism of interaction
with each type of nucleic acid ligand.
These data shed light on both gene regulation in the Lyme spirochete
and functional mechanisms of the widely-distributed PUR-domain.
Posted by sparkle7 (Member # 10397) on :
It's a bit hard to understand. I'm tired... Is there a layman's explanation anywhere?
Posted by lpkayak (Member # 5230) on :
im missing the relationship to morgellons...i will forward it to friend who knows more
Posted by Pinelady (Member # 18524) on :
They are explaining the gene sharing stealth of spirochetal disease. IT can share its genes with whatever it wants to survive. IT is what you are infected with, what junk GM genes you ingest, and what infectious antigens you add to the spirochetal prion protein mix. Which is what causes Autism, etc.
http://www.ncbi.nlm.nih.gov/pubmed/23841456 BMC Evol Biol. 2013 Jul 11;13(1):146. [Epub ahead of print] Coexistence of ribbon and helical fibrils originating from hIAPP20�29 revealed by quantitative nanomechanical atomic force microscopy
Uncontrolled misfolding of proteins leading to the formation of amyloid deposits is associated with more than 40 types of diseases, such as neurodegenerative diseases and type-2 diabetes. These irreversible amyloid fibrils typically assemble in distinct stages. Transitions among the various intermediate stages are the subject of many studies
Cys was made in the lab for use in making vaccines so they did not have to constantly be using live organisms in the making of them. It is the genetic equivilent of e coli used in many vaccines for animals and humans.
They used e coli strains most likely from bovine source and then compounded the problem by giving it back to them genetically altered in HeLa/Spirochetal infected cells.
Until now they did not know that MS/ALS/Alzheimers/Parkinsons/Lymphoma/Arthritis'/etc. etc. etc. are all suspect to be caused by actual multiple infections that cannot be seen until enough are killed to enable tests to pick up.
University of KY. has identified 3 of Borrelia's proteins to be prion like in their stealth ability.
http://nar.oxfordjournals.org/content/38/16/5443.full EbfC preferentially binds the palindromic sequence GTnAC, where �n� can be any base, with all erp Operator 2 regions containing two adjacent EbfC-binding sites "What this says is exactly what it means".
The n may be any peice of any organism folded within the protein to cause disease.
They are looking right now to see what all it is capable of hiding. Which is the cause of all our "Syndromes" of today.
Mad Cow was a myth of epic proportion, by the failures of govt. and acceptance to leave it as such to continue profits for all the symptoms by refusal to seek the cause and treatments to cure. Because they def. KNEW.
About 25% of the polyrod structures have attached polyhook structures in the FlgG-G65V mutant background (Fig. 2B). Unexpectedly, the polyrods with attached hooks exhibit a peak in measured lengths. The significance of this result is not clear at this time. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1973146/ Genes Dev. 2007 September 15; 21(18): 2326�2335. doi: 10.1101/gad.1571607 PMCID: PMC1973146 The mechanism of outer membrane penetration by the eubacterial flagellum and implications for spirochete evolution
We have a but a very small window of opportunity to change our world for a much better one. BUT it will not come without the people being the ones to DEMAND IT NOW.
Posted by droid1226 (Member # 34930) on :
I find it interesting Morgellon's is considered a delusional parasitic infection that causes open sores.
Posted by ms dixie (Member # 40818) on :
There are MANY health boards where testimonies are found of cures or improvement with fenbendazole , a substitute easily obtained for albendazole . ONCE AGAIN, PARASITES !!!
Posted by Razzle (Member # 30398) on :
So quorum sensing should be blocked to prevent Bb from sharing genetic material with other organisms...
Oregano, Basil, Thyme, etc., are good for this, and should be included in one's diet on a regular basis, if possible.
Posted by sparkle7 (Member # 10397) on :
Thanks Pinelady. I'll come back & read everything when I have time. This is pretty much what I already knew but not in such scientific details. I think alot of these "out there" illnesses are related. I think there's alot of misdiagnosis & misinformation surrounding all of this, as well.
Posted by Pinelady (Member # 18524) on :
Borrelia is not the only organisms who have gained the same gene sharing stealth abilities as spirochetes. Very profound article.
The Trypanosoma brucei genome accommodates an archive of thousands of VSGs, located mainly in subtelomeric arrays on conventional chromosomes [8] and in the subtelomeres of a pool of approximately 100 minichromosomes [9] snip Many pathogens, including Anaplasma spp. [13], Borrelia burgdorferi [14], Neisseria gonorrhoeae [15], Treponema pallidum [16], Mycoplasma spp. [17] and Babesia bovis [18],
undergo a process of segmental gene conversion (SGC) that introduces variation in the expressed antigen.
In this process, conversion occurs within the open reading frame, producing a gene
that contains segments from two or more donors.
By varying only the immunodominant region of an antigen, SGC can make efficient use of a small genome,
and can potentially generate vast combinatorial diversity