My experience with testing is pretty typical and it showed a specialty lab better than two tier. Furthermore, elisa negative and both parts of WB missing only one band to be CDC positive. This with documented coinfection elsewhere, tickbite, symptoms. Can't tell me the two tier is better. Most doctors would have turned me away with no treatment based on the two tier testing.
Furthermore,they don't discuss knowledgeably seronegative people, the problems with antibody testing, most strains not covered by testing, and known species excluded. Lab variability is just one problem. There are many others.