Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA.
The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil.
At the S�o Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled.
Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively.
Using 16S-23S rRNA intergenic transcribed spacer (ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples.
Bartonella seroreactive and/or PCR positive blood samples (n=8) were inoculated into a liquid pre-enrichment growth medium (BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates.
PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog (ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806).
B. henselae and B. vinsonii subsp. berkhoffii (pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs.
We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species.
The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world.
To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.
PMID: 17583666 [PubMed - indexed for MEDLINE]
-------------------- Note: I'm NOT a medical professional. The information I share is from my own personal research and experience. Please do not construe anything I share as medical advice, which should only be obtained from a licensed medical practitioner. Posts: 4881 | From Middlesex County, NJ | Registered: Jul 2006
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Clarissa
Frequent Contributor (1K+ posts)
Member # 4715
posted
So do you think we need to get our dogs' tested? My vet said if I brought in a blood test kit, she'd be happy to do it.
I'm going to ask my present LLMD if animal test kits exist.
I'm "trying" to be ignorant to this fact but it's probably better to face it head-on...especially after all the hell I've been through with Bart.
AliG: What are you going to do about your aardvark? My mini-doxy Spencer is the next Casanova.
Keebler
Honored Contributor (25K+ posts)
Member # 12673
posted
-
Ali,
Thanks for your recent storm of activity with some research articles. Very interesting . . . more homework but looking forward to going through your recent posts.
I have many files on my Mac desktop labeled "read" "to read" "study" etc. It would be nice if a computer could just read them into our brains, eh?
AliG
Frequent Contributor (1K+ posts)
Member # 9734
posted
Clarissa,
They test cats for Bartonella, No? Why not dogs? I probably should test my "aardvark" since I can't escape that foot-long tongue.
Keebler,
You're very welcome for the articles. I think my files are "read", "re-read" and "re-re-read". LOL
Maybe someday we'll be able to have chips installed in our brains & just download all the info. It sure is nice to dream!
-------------------- Note: I'm NOT a medical professional. The information I share is from my own personal research and experience. Please do not construe anything I share as medical advice, which should only be obtained from a licensed medical practitioner. Posts: 4881 | From Middlesex County, NJ | Registered: Jul 2006
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I think my files are "read", "re-read" and "re-re-read". ![[Roll Eyes]](rolleyes.gif)
LOL![[hi]](graemlins/hi.gif)
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