LymeNet Flash: Concentration of sFas and sFasL in the supernatant of PBMC culture from LLB patients

LymeNet on Facebook

LymeNet on Twitter

Follow @LymeDiseaseNet

The Lyme Disease Network receives a commission from Amazon.com for each purchase originating from this site.

When purchasing from Amazon.com, please
click here first.

Thank you.

Dedicated to the Bachmann Family

LymeNet needs your help:
LymeNet 2020 fund drive

The Lyme Disease Network is a non-profit organization funded by individual donations.

»	LymeNet Flash » Questions and Discussion » Medical Questions » Concentration of sFas and sFasL in the supernatant of PBMC culture from LLB patients

UBBFriend: Email this page to someone!

Author

Topic: Concentration of sFas and sFasL in the supernatant of PBMC culture from LLB patients

AliG
Frequent Contributor (1K+ posts)
Member # 9734

posted

Przegl Epidemiol. 2007;61(1):51-8.Links

{Concentration of sFas and sFasL in the supernatant of PBMC culture from the patients with late lyme borreliosis}
[Article in Polish]

Grygorczuk S, Chmielewski T, Zajkowska J, Pancewicz S, Swierzbińska R, Kondrusik M, Tylewska-Wierzbanowska S, Hermanowska-Szpakowicz T.

Klinika Chor�b Zakaźnych i Neuroinfekcji Akademii Medycznej w Białymstoku. [email protected]

OBJECTIVE: Persistent, inadequate inflammatory response present in late Lyme borreliosis may be driven by activated T lymphocytes.

We estimated synthesis of extracellular proteins: soluble Fas receptor (sFas) and its ligand (sFasL), which might protect T lymphocytes from physiologic apoptosis, in the peripheral blood mononuclear cell (PBMC) culture from patients with late borreliosis.

METHODS: Lyme borreliosis group (LB) consisted of 20 patients with Lyme borreliosis present for >6 months.

Six patients without any active infection constituted the control group (K).

PBMC were incubated for 7 days with phytohemaglutinine or suspension of Borrelia afzeli (Ba), B. garinii (Bg) and B. burgdorferi sensu stricto (Bss) spirochetes.

sFas and sFasL concentrations were measured in the culture supernatant with ELISA.

RESULTS: In LB mean sFasL concentration was increased significantly under stimulation with phytohemaglutinine, Ba and Bg, and, with borderline significance, with Bss, in comparison with unstimulated culture.

sFas also tended to increase, which was significant with phytohemaglutinine and borderline with Bg.

In K sFas and sFasL was not significantly increased under antigenic stimulation.

CONCLUSIONS: Increased synthesis of antiapoptotic factors by PBMC from patients with late borreliosis incubated with B. burgdorferi antigens may suggest impaired apoptosis of T lymphocytes contributing to persistent inflammatory response in this patients.

PMID: 17702439 [PubMed - indexed for MEDLINE]

--------------------
Note: I'm NOT a medical professional. The information I share is from my own personal research and experience. Please do not construe anything I share as medical advice, which should only be obtained from a licensed medical practitioner.

Posts: 4881 | From Middlesex County, NJ | Registered: Jul 2006 | IP: Logged |

Printer-friendly view of this topic

The Lyme Disease Network is a non-profit organization funded by individual donations. If you would like to support the Network and the LymeNet system of Web services, please send your donations to:

The Lyme Disease Network of New Jersey
907 Pebble Creek Court, Pennington, NJ 08534 USA
http://www.lymenet.org/