Ticks and mosquitoes as vectors of Borrelia burgdorferi s. l. in the forested areas of Szczecin.
Kosik-Bogacka DI, Kuźna-Grygiel W, Jaborowska M. Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstancow Wielkopolskich Av. 72, 70-111 Szczecin, Poland. [email protected]
The aim of the study was to determine the infection level of adult forms and larvae of ticks and mosquitoes with Borrelia burgdorferi in the forested areas of Szczecin. A total of 1699 ticks Ixodes ricinus, including 1422 nymphs, 277 adult forms and 2862 mosquito females representing the genera Aedes (89.6%) and Culex (10.4%) were collected between the years 2004 and 2005. A further 3746 larvae and 1596 pupae of Culex pipiens pipiens were colleted from water bodies. Borrelia burgdorferi s. l. was detected in the arthropods by the method of indirect immunofluorescence assay (IFA).
A positive immunological reaction was detected in 16.6% of the adult forms and in 16.5% of the nymphs of Ixodes ricinus. Spirochetes were also detected in 1.7% of mosquito females, 3.2% of larvae and in 1.6% of pupae of Culex pipiens pipiens.
The results of the present study confirm that contact with ticks constitutes the main risk of contracting Lyme disease, although mosquitoes play a role as vectors as well.
PMID: 18274258 [PubMed - in process]
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Borrelia burgdorferi sensu lato infection in mosquitoes from Szczecin area.
Kosik-Bogacka DI, Kuzna-Grygiel W, Gornik K. Chair and Department of Biology and Medical Parasitology, Pomeranian Medical University, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland. [email protected]
The aim of the study was to determine the level of infection in mosquitoes with spirochetes Borrelia burgdorferi sensu lato in the woody areas of Szczecin. The mosquitoes were collected from May to September 2003.
The spirochetes, Borrelia burgdorferi s. l., present in mosquitoes were detected in mosquitoes with indirect immunofluorescence assay (IFA) using rabbit anti-Borrelia burgdorferi antibodies and goat anti-rabbit IgG marked with fluorescein isocyanate (FITC).
A total of 1557 females and 58 males were collected. They represented the genera Aedes (63%) and Culex (37%). The infection level of the mosquitoes from the area studied amounted to 1.7%.
The results of the present study confirm the potential of these arthropods to spread Lyme borreliosis.
PMID: 17044261 [PubMed - indexed for MEDLINE]
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Isolation of Borrelia afzelii from overwintering Culex pipiens biotype molestus mosquitoes.
* Zakovska A, Capkova L, Sery O, Halouzka J, Dendis M. Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic. [email protected]
A total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus Culex, Aedes and Anopheles were collected during the period March 2000-April 2001 from the locality of Vysoke Myto (Eastern Bohemia, Czech Republic). They were examined by dark field microscopy for the presence of spirochetes.
The motile spirochetes were observed in 4.2% of all species of investigated mosquitoes. One spirochetal strain out of the 8 isolation attempts (BRZ14) was obtained (cultivation rate was 12.5 %) and the spirochetal strain was then successfully cultivated and identified using PCR for the presence of Borrelia burgdorferi s.l., and subsequently with the RFLP as genomospecies Borrelia afzelii.
This strain was derived from overwintering Culex (Culex) pipiens biotype molestus female mosquitoe. This is apparently one of the sporadic cases of the occurrence of pathogenic borreliae in haematophagous arthropods, other than Ixodes ricinus complex ticks.
PMID: 17199258 [PubMed - indexed for MEDLINE]
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Positive findings of Borrelia burgdorferi in Culex (Culex) pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method. * Zakovska A, Nejedla P, Holikova A, Dendis M. Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic. [email protected]
After first finding Borrelia in the midgut of imago mosquitoes, we concentrated on the presence of Borrelia in mosquito development stages--larvae of the third or fourth instar. In the summer season in the years of 2000-2001 a total of 439 Culex (Culex) pipiens pipiens larvae were collected from a barrel of rainwater in the Obrany holiday area of Brno city (East Moravia, Czech Republic). The larvae midgut was observed under dark-field microscopy. Ten DFM positive samples (2.28%) were further analysed using the single-tube nested PCR method for the presence of flagellum DNA sequence specific for Borrelia burgdorferi sensu lato, of which 5 were positive. Borrelian positivity of Culex (C.) pipiens pipiens larvae was 1.14%. One spirochete isolated strain in BSK-H medium was obtained. PCR detection for borrelian DNA of the isolated strain was negative.
From these results we can conclude that a low percentage Borreliae can be also found in mosquito larvae and are likely to survive into imago stage.
PMID: 12498597 [PubMed - indexed for MEDLINE]
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Detection of Borrelia burgdorferi sensu lato in mosquitoes (Culicidae) in recreational areas of the city of Szczecin.
* Kosik-Bogacka D, Bukowska K, Kuzna-Grygiel W. Department of Biology and Medical Parasitology, Pomeranian Medical University, 70-111 Szczecin, al. Powstancow Wielkopolskich 72, Poland. [email protected]
The mosquitoes were caught within the period of their highest activity, i.e. June August 2001, in recreational areas of the city of Szczecin.
Spirochetes, Borrelia burgdorferi sensu lato were detected in mosquitoes with the aid of the method of indirect immunofluorescence (IFA) using rabbit anti-Borrelia burgdorferi antibodies and goat anti-rabbit IgG marked with fluorescein isothiocyanate (FITC).
A total of 639 mosquito females representing genera Aedes (99.1%) and Culex (0.9%) were collected. The mean value of the infection rate of mosquitoes from the area studied was 1.25%. The highest infection rate was recorded in June (3.2%), while the lowest -- in July (0.6%).
All mosquitoes infected with Borrelia burgdorferi spirochetes belonged to the genus Aedes. The results of the present study confirm a potential role of those arthropods in epidemiology of Lyme borreliosis
PMID: 12088398 [PubMed - indexed for MEDLINE]
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Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Brno.
During the years 1995-1996, a total of 1,743 overwintering Culex pipiens biotype molestus female mosquitoes were tested for the presence of spirochetes in several localities in South Moravia, Czech Republic.
The spirochetes were observed in 5% of the mosquitoes investigated. One of the five isolated strains of spirochetes (BR-84) was identified as Borrelia afzelii.
The potential role of mosquitoes in the ecology and epidemiology of Lyme disease (LD) borreliae should be further investigated.
PMID: 10885843 [PubMed - indexed for MEDLINE]
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treepatrol
Honored Contributor (10K+ posts)
Member # 4117
posted
Thanks
-------------------- Do unto others as you would have them do unto you. Remember Iam not a Doctor Just someone struggling like you with Tick Borne Diseases.
AliG
Frequent Contributor (1K+ posts)
Member # 9734
posted
What an excellent collection!
Thanks so much for this Ima!
I hope Geneal sees this. I believe she's been having problems with her Parish mosquito control believing ketes can be transmitted by squeets.
Up for Geneal!!!!! (now I have to find Geneal )
-------------------- Note: I'm NOT a medical professional. The information I share is from my own personal research and experience. Please do not construe anything I share as medical advice, which should only be obtained from a licensed medical practitioner. Posts: 4881 | From Middlesex County, NJ | Registered: Jul 2006
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Michelle M
Frequent Contributor (1K+ posts)
Member # 7200
posted
Thanks, Ima. These are very informative.
I wonder why there are no U.S. studies. Or are there? I doubt our mosquitoes are much different.
Michelle
Posts: 3193 | From Northern California | Registered: Apr 2005
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D Bergy
Frequent Contributor (1K+ posts)
Member # 9984
posted
Wonderful.
Do you know how many mosquitoes we have in Northern MN? Apparently, in our area the mosquitoes must not carry, or be able to transmit the bacteria. We all would be infected in a years time.
D Bergy
Posts: 2919 | From Minnesota | Registered: Aug 2006
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