Topic: Characterization of SP41, a surface protein of Brucella associated with adherence
treepatrol
Honored Contributor (10K+ posts)
Member # 4117
posted
Characterization of SP41, a surface protein of Brucella associated with adherence and invasion of host epithelial cells Elsa I. Casta�eda-Rold�n, 1 Safia Ouahrani-Bettache, 2 Zeus Salda�a, 3 Fabiola Avelino, 1 Mar�a A. Rend�n, 3 Jacques Dornand 2 and Jorge A. Gir�n 3* 1 Centro de Investigaciones en Ciencias Microbiol�gicas, Benem�rita Universidad Aut�noma de Puebla, Ciudad Universitaria, Puebla, 72570, M�xico. 2 INSERM U431 Universit� de Montpellier II, 34075 Montpellier, France. 3 Department of Immunobiology, University of Arizona, Tucson, AZ 85724, USA. ABSTRACT
Summary Brucella is an invasive organism that multiplies and survives within eukaryotic cells. The brucellae are able to adhere to the surface of cultured epithelial cells, a mechanism that may facilitate penetration and dissemination to other host tissues. However, no adhesins that allow the bacteria to interact with the surface of epithelial cells before migration within polymorphonuclear leukocytes, monocytes and macrophages have been described. Here, we show that Brucella surface proteins (SPs) with apparent molecular masses of 14, 18 and 41 kDa bound selectively to HeLa cells. However, only antibodies directed against the 41 kDa surface protein (SP41) inhibited in dose-response manner, bacterial adherence and invasion of HeLa cells. HeLa cells treated with neuraminidase did not bind SP41, suggesting the involvement of cellular sialic acid residues in this interaction. Biochemical analysis of SP41 revealed that this protein is the predicted product of the ugpB locus, which showed significant homology to the glycerol-3-phosphate-binding ATP-binding cassette (ABC) transporter protein found in several bacterial species. SP41 appears to be exposed on the bacterial surface as determined by immunofluorescence and immunogold labelling with anti-SP41 antibody. An isogenic ΔugpB mutant showed a significant inhibitory effect on Brucella adherence and invasion of human cultured epithelial cells and this effect could be reversed by restoration of the ugpB on a plasmid. Lastly, we also show that most of the sera from individuals with acute brucellosis, but not sera obtained from healthy donors or patients with chronic brucellosis, mount antibody reactivity against SP41, suggesting that this protein is produced in vivo and that it elicits an antibody immune response. These data are novel findings that offer new insights into understanding the interplay between this bacterium and host target cells, and identify a new target for vaccine development and prevention of brucellosis. http://www3.interscience.wiley.com/journal/118585323/abstract
Now this is interesting considering bio weapon engineering they used brucellosis and spirochetes huh.
-------------------- Do unto others as you would have them do unto you. Remember Iam not a Doctor Just someone struggling like you with Tick Borne Diseases.
The Lyme Disease Network is a non-profit organization funded by individual donations. If you would like to support the Network and the LymeNet system of Web services, please send your donations to:
The
Lyme Disease Network of New Jersey 907 Pebble Creek Court,
Pennington,
NJ08534USA http://www.lymenet.org/
UBBFriend: Email this page to someone!
Printer-friendly view of this topic