Intracellular Pathogens Research Laboratory, Center for Comparative Medicine and Translational Research, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, United States.
Although an organism primarily associated with non-clinical bacteremia in domestic cattle and wild ruminants, Bartonella bovis was recently defined as a cause of bovine endocarditis.
The purpose of this study was to develop a B. bovis species-specific PCR assay that could be used to confirm the molecular prevalence of Bartonella spp. infection.
Blood samples from 142 cattle were tested by conventional PCR targeting the Bartonella 16S-23S intergenic spacer (ITS) region.
Overall, Bartonella DNA was detected in 82.4% (117/142) of the cattle using either Bartonella genus primers or B. bovis species-specific primers.
Based upon size, 115 of the 117 Bartonella genus ITS PCR amplicons were consistent with B. bovis infection, which was confirmed by PCR using B. bovis species-specific primers and by sequencing three randomly selected, appropriately sized Bartonella genus PCR amplicons.
By DNA sequencing, Bartonella henselae was confirmed as the two remaining amplicons, showing sequence similarity to B. henselae URBHLIE 9 (AF312496) and B. henselae Houston 1 (NC_005956), respectively.
Following pre-enrichment blood culture of 12 samples in Bartonella alpha Proteobacteria growth medium (BAPGM) B. henselae infection was found in another three cows.
Four of the five cows infected with B. henselae were co-infected with B. bovis.
To our knowledge this study describes the first detection of B. henselae in any large ruminant species in the world and supports the need for further investigation of prevalence and pathogenic potential of B. henselae and B. bovis in cattle.
-------------------- My biofilm film: www.whyamistillsick.com 2004 Mycoplasma Pneumonia 2006 Positive after 2 years of hell 2006-08 Marshall Protocol. Killed many bug species 2009 - Beating candida, doing better Lahey Clinic in Mass: what a racquet! Posts: 830 | From Mass. | Registered: Aug 2006
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Angelica
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posted
I wonder if the cattle have bartonella how many of them have Lyme as well?
There is a ex cattle rancher here (Calif.) who has lyme disease. He told me years ago in Wisconsin there was a big out break in many of the cattle there where they all had bartonella or maybe he said Lyme I forget which disease it was but he did mention it was a big well known outbreak.
Where I grew up I found out recently it is a hot spot for lyme. Briefly the land right next to our house was used as a cow pasture by a rancher before it became open space. I wonder if the neighborhood has the cows to thank for the turning the area into a lyme hot spot. There were many new flies and horse flies once they moved the cattle in.
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posted
Yes, I'm assuming high heat kills infections in the milk and the meat? Anyone know for sure?
Posts: 13116 | From San Francisco | Registered: May 2006
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blaze
Unregistered
posted
It's not the cows' faults. Please don't blame the cows.
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Melanie Reber
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Member # 3707
posted
Can we assume that these cattle were from NC? Anyone able to read the full article?
Posts: 7052 | From Colorado | Registered: Mar 2003
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sparkle7
Frequent Contributor (5K+ posts)
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posted
Were all of the cattle blood samples taken from the same area? I looked up the study & it didn't say.
I did see a similar study published on another website & it specifically stated they were NC cows.
PCR DETECTION OF BARTONELLA BOVIS IN THE BLOOD OF NORTH CAROLINA BEEF CATTLE
NA Cherry*, RG Maggi, AL Kennedy, EB Breitschwerdt, Intracellular Pathogens Research Laboratory, College of Veterinary Medicine, North Carolina State University
Although an infrequent cause of bovine endocarditis, Bartonella bovis is an organism primarily associated with non-clinical bacteremia in domestic cattle and wild ruminants.
The zoonotic potential of B. bovis increased substantially when this species was implicated as the cause of human bacillary angiomatosis. The purpose of this study was to determine the molecular prevalence of Bartonella spp. among beef cattle in North Carolina.
EDTA anti-coagulated blood samples from 142 cattle from three different farms were tested by conventional PCR using Bartonella 16S-23S intergenic spacer (ITS) primers.
Bartonella DNA was detected in 79.6% (113/142) of the cattle and there was no difference in molecular prevalence among the 3 farms. Subsequently, newly designed B. bovis-specific primers identified a molecular prevalence of 76.1% (108/142 samples).
Of three B. bovis amplicons selected for sequencing confirmation, there was between 98 and 99% homology with B. bovis, accession number AY116638, for each of these samples.
Sequences from the only two smaller ITS amplicons had 99.8 and 99.7% homology, respectively, with B. henselae Houston 1 (accession number NC005956).
There was 95% agreement among the two PCR assays. Following inoculation of blood from 12 samples into Bartonella alpha Proteobacteria growth medium (BAPGM) and subculture onto blood agar plates, neither B. bovis nor B. henselae isolates were obtained.
To our knowledge this study reports the first detection of B. henselae in any large ruminant in the world.
Because B. henselae causes a wide variety of illness in animals and humans alike, including Cat Scratch Disease, infective endocarditis, lymphadenopathy, bacillary angiomatosis and peliosis hepatis, this novel finding supports the need for further investigation of infection with this Bartonella species in cattle.
Although an obviously well-adapted intravascular bacteria in beef cattle, the role of B. bovis in bovine diseases such as shipping fever respiratory complex or fescue toxicity should be considered.
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On google there were lot of other studies about bart in dog saliva, biting flies in CA, French cows & cat fleas, etc...
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I also found this -
In this study - they found the presence of a "nano-bacteria" along with the bart....
Infection with Bartonella weissii and Detection of Nanobacterium Antigens in a North Carolina Beef Herd
Edward B. Breitschwerdt,* Sushama Sontakke, Allen Cannedy, Susan I. Hancock, and Julie M. Bradley
To our knowledge, data related to Nanobacterium infection in cattle are not available from United States.
As Nanobacteria purportedly share similar surface antigens with Bartonella spp., there may be serologic cross-reactivity; however, the extent to which cross-reactivity occurs has not been well characterized (11, 12).
Because of the potential for serologic cross-reactivity among these two organisms, we tested a subgroup of the herd with a commercially available antigen ELISA test kit.
Surprisingly, 100% (22 of 22) of the cattle tested in this herd had very high levels of circulating nanobacterial antigens.
In contrast, 29 of 32 dog samples did not contain detectable levels of nanobacterial antigens. Assuming the specificity of the monoclonal antibody for the detection of nanobacterial antigens, these results would support a high rate of nanobacterial infection in this herd.
Whether a correlation exists between the detection of nanobacteria antigens and infection with B. weissii in this herd was not determined, due to the presumed relative insensitivity of bartonella blood culture as used in this study.
In humans and experimentally infected laboratory animals, Nanobacteria can cause chronic infection (1, 8, 12).
For example, Nanobacteria were reportedly isolated from the blood of one person for five years, despite the concurrent presence of antibodies.
Following experimental infection of rabbits with Nanobacteria derived from fetal bovine sera, organisms can be isolated from urine or cerebrospinal fluid for up to 1 year (1, 12).
Most recently, a role for Nanobacterium in the development of human kidney stones and polycystic kidney disease has been proposed (8, 10).
Future studies should examine the potential relationship between exposure to and infection with Bartonella and Nanobacterium species, the extent to which these organisms might contribute to disease manifestations in cattle and the extent to which cattle might serve as a reservoir for human infection.
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This doesn't sound good to me....
Posts: 7772 | From Northeast, again... | Registered: Oct 2006
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Melanie Reber
Frequent Contributor (5K+ posts)
Member # 3707
posted
Thanks Sparkle,
That actually was the same study as the one TC listed...but your PDF contained a bit more info.
Posts: 7052 | From Colorado | Registered: Mar 2003
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NanaDubo
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Member # 14794
posted
Yikes - some of my friends are really into the raw milk and yogurt thing from a nearby farm. This has bothered me but now I think I need to talk to them about it.
Posts: 1129 | From Maine | Registered: Feb 2008
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sparkle7
Frequent Contributor (5K+ posts)
Member # 10397
posted
re: "very high levels of circulating nanobacterial antigens"
Does anyone know what this means?
PS - Nana - you may want to suggest that the farm test the cows for Bart... Seems like raw milk has some benefits but it would be good to find out if the cows are healthy - especially if you know the farmer.
Posts: 7772 | From Northeast, again... | Registered: Oct 2006
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tdtid
Frequent Contributor (1K+ posts)
Member # 10276
posted
Definitely one of those kinds of articles that makes you sit and ponder.
I'm not sure all the way WHAT it means from a deeper level, but not a good thing no matter what.
Thanks for sharing.
Cathy
-------------------- "To Dream The Impossible Dream" Man of La Mancha Posts: 2638 | From New Hampshire | Registered: Oct 2006
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NanaDubo
Frequent Contributor (1K+ posts)
Member # 14794
posted
Thanks Sparkle - good idea and I will mention it to my friends.
So....in terms of meat - anyone know if this stuff dies at a certain temperature? I gave up eating any meat that didn't come from pasture fed, organic sources a long time ago due to the hormones and antibiotics.
Now pasture fed just makes me think of ticks Posts: 1129 | From Maine | Registered: Feb 2008
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Tincup
Honored Contributor (10K+ posts)
Member # 5829
posted
Hey nana..
I had to laugh. Yes.. pasture fed or whatever.. could mean ticks.
Interesting thought to me a friend mentioned...
Cats, barns, mice and cows...
Think about it... they all go together like a horse and carriage or peanut butter and jelly.
So it stands to reason that it could be spread from one thing to another.... mice- to cats- to cows. All in close quarters.
You asked if it was killed during high heat.. or cooking I assume?
That was the first thing I asked too. researched it for a long time actually.. found no direct answers.
But I did find that lab people.. to kill Bartonella organisms to use in experiments... boiled the bartonella for ONE HOUR.
I know I sure don't boil my meat at that high a temperature for that long.
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