COTES A Phanotron (hot-cathode gas filled tube) diode was placed in strategic proximity to the culture on the slide to maximize exposure. Sweeps of frequencyspecific electric pulses were broadcast from the plasma tube while the culture was being observed at 1000X with a phase contrast microscope. Morphological changes in individual spirochetes and the biofilm were recorded. Heuristic methodologies formed the basis for the exploration sweeps. Initial frequencies were determined using calculations that took into account genomic characteristics (including genome length and form).
COTES frequency sweeps 69 day culture: Part 1a: 10.01 - 10.99 kHz in 1Hz increments (with included sweeps of [6870, 6863, 4200, 2050, 2016, 1520, 1455, 46866, 46851, 34170, 34112, 920, 884, 800, 797, 758, 673, 625, 615, 605, 432, 345, 344, 388, 254]- 3 min. Each, 68% duty cycle
Part 1b: 1330.57 � 1360.57 in 1Hz increments- 3 min. each, 60% duty cycle Circa for part 1: 270 minutes
Part 2a: [442, 464, 490, 498]-3 min. each, 30% duty cycle; [619, 673, 727, 733, 784, 757, 783, 767, 801, 842]-3 min. each 70% duty cycle. Part 2b: 72.5 - 73 kHz in 100Hz increments- 5 min. each, 60% duty cycle Circa for part 2: 100 minutes. See photo dates: 1/13/2011 and 1/14/2011
76 day culture: Part 1a: 72.1 -73.0 kHz in 100Hz increments- 5 min. each, 60% duty cycle Part 1b: 73.0 -74.0 kHz in 100Hz increments- 5 min. each, 60% duty cycle Circa for part 1: 110 minutes
Part 2a: 72.0 -73.0 kHz in 100Hz increments- 5 min. each, 70% duty cycle
Part 2b: 73.1 -74.0 kHz in 100Hz increments- 5 min. each, 70% duty cycle
Part 2c: 73.8 -74.8 kHz in 50HZ increments- 5 min. each, 50% duty cycle Circa for part 2: 195 minutes. See photo date: 1/20/2011
95 day culture: Part 1a: 73.8 -74.8 Hz in 50HZ increments- 5 min. each, 50% duty cycle
also - the bottom photo on holland's poster shows "fewer RBs were noted when the culture was exposed to same oscillatory explorations." I wonder if they are dying or ???
[ 01-06-2013, 01:19 PM: Message edited by: cottonbrain ]
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cottonbrain
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posted
any takers?
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D Bergy
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The duty cycle of the square wave is given. I am not sure why he is always changing it?
There is no reference to gating, although I suspect he is using gating.
Dan
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cottonbrain
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posted
Dan, thank you. you are just the person who can help me here!
the poster says: 10.01 - 10.99 kHz in 1Hz increments
does this mean he used a gate rate of one hertz?
I tried running channel 466 on the GB4000 (it uses most of hollands freqs) with the duty cycles that he gave. During the rifing i had only mild response, but about an hour later I had some herxing. Usually i get immediate response on a freq., esp. when I use the 2016 freq that you recommend, Dan.
anybody else try this?
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D Bergy
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posted
It sounds like a sweep using 1 Hertz increments.
A gate rate would be a higher number.
Your response to the auto channel is pretty interesting. I will have to see what that auto channel is all about.
Dan
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