BACKGROUND: Treponema denticola is an oral spirochete involved in the pathogenesis and progression of periodontal disease.
Of its virulence factors,
the major surface protein (MSP) plays a role in the interaction between the treponeme and host.
To understand the possible evolution of this protein, we analyzed the sequence of the msp gene in 17 T. denticola positive clinical samples.
METHODS: Nucleotide and amino acid sequence of MSP have been determined by PCR amplification and sequencing in seventeen T. denticola clinical specimens
to evaluate the genetic variability and the philogenetic relationship of the T.denticola msp gene
among the different amplified sequence of positive samples.
In silico antigenic analysis was performed on each MSP sequences to determined possible antigenic variation.
RESULTS: The msp sequences showed
two highly conserved 5' and 3' ends
and a central region that varies substantially.
Phylogenetic analysis categorized the 17 specimens into 2 principal groups, suggesting a low rate of evolutionary variability and an elevated degree of conservation of msp in clinically derived genetic material.
Analysis of the predicted antigenic variability between isolates, demonstrated that the major differences lay between amino acids 200 and 300.
CONCLUSION: These findings showed for the first time, the nucleotide and amino acids variation of the msp gene in infecting T.denticola in vivo.
This data suggested that the antigenic variability found in to the MSP molecule,
may be an important factor involved in immune evasion by T. denticola.
-------------------- Suspected Lyme 07 Test neg One band migrating in IgG region unable to identify.Igenex Jan.09IFA titer 1:40 IND IgM neg pos 31 +++ 34 IND 39 IND 41 IND 83-93 + DX:Neuroborreliosis Posts: 5850 | From Kentucky | Registered: Dec 2008
| IP: Logged |
Pinelady
Frequent Contributor (5K+ posts)
Member # 18524
posted
Why don't they just tell people--
In order to keep dentists in business we cannot give you enough antibiotics to get rid of the organisms that destroy your teeth and eat your bones? Go figure.
-------------------- Suspected Lyme 07 Test neg One band migrating in IgG region unable to identify.Igenex Jan.09IFA titer 1:40 IND IgM neg pos 31 +++ 34 IND 39 IND 41 IND 83-93 + DX:Neuroborreliosis Posts: 5850 | From Kentucky | Registered: Dec 2008
| IP: Logged |
onbam
Unregistered
posted
I'm not sure it's something that you can get rid of. I think that, like the rest of the naturally occurring oral bacteria, good dental hygeine keeps it in check.
IP: Logged |
The Lyme Disease Network is a non-profit organization funded by individual donations. If you would like to support the Network and the LymeNet system of Web services, please send your donations to:
The
Lyme Disease Network of New Jersey 907 Pebble Creek Court,
Pennington,
NJ08534USA http://www.lymenet.org/
UBBFriend: Email this page to someone!
Printer-friendly view of this topic