lyme in Putnam
Frequent Contributor (1K+ posts)
Member # 11561
posted
Why does the CDC have 30 as one of their 5 banns to be positive for igg and not igenex? Ive always had 31 from igenex 5 times, this time not a 31, just a 30. ???
-------------------- He took u to it, He'll you through Posts: 2837 | From NE. | Registered: Apr 2007
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joalo
Frequent Contributor (1K+ posts)
Member # 12752
posted
Up.
-------------------- Sick since January 1985. Misdiagnosed for 20 years. Tested CDC positive October 2005. Treating since April 2006. Posts: 3228 | From Somewhere west of the Mississippi | Registered: Aug 2007
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posted
For the same reason(s?) IGeneX doesn't use the 18, 28, 45, 58 and 66 bands.
The bands IGeneX uses are the bands they found to be "highly significant markers for infection compared to both Groups 2 and 3."
Group 2 were patients with tick-borne infections other than lyme. Group 3 were the normal controls.
Posts: 426 | From Berkeley, CA | Registered: Feb 2009
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lyme in Putnam
Frequent Contributor (1K+ posts)
Member # 11561
posted
Thx.
-------------------- He took u to it, He'll you through Posts: 2837 | From NE. | Registered: Apr 2007
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susank
Frequent Contributor (1K+ posts)
Member # 22150
posted
I heard this thread calling to me. Re: Igenex
Chuck posted bands I have tested positive for over time - to include band 30 - and exclude band 45. But since Igenex only puts IND on their ** bands - who knows - maybe I had an IND 45 - will never know. But that is beside the point.
But is why I wish Igenex would provide pictures of our results in addition to their interpretation.
Igenex considers bands 30 and 31 to be closely associated IIRC. My first test I had an IGG 31 IND. Third test I had IGM 30+ along with IGG 31 IND. That is the only time band 30 has shown for me. Igenex suggested epitope tests for the 31's and the 30. All negative.
Chuck - your group 2 concerns me. One could have Bart and Lyme - and due to having Bart - be considered Lyme neg - because of cross-reacting bands?
ie some bands could be Bart and Lyme -so if in doubt - call it non-specific - which could result in overall result as neg. for LD? That's real logical. That is the best anyone can do with these tests?
Another reason I guess we hear "it's a clinical diagnosis" so often - because many folks have co-infections.
quote:Originally posted by susank: I heard this thread calling to me. Re: Igenex
Chuck posted bands I have tested positive for over time - to include band 30 - and exclude band 45. But since Igenex only puts IND on their ** bands - who knows - maybe I had an IND 45 - will never know. But that is beside the point.
But is why I wish Igenex would provide pictures of our results in addition to their interpretation.
Igenex considers bands 30 and 31 to be closely associated IIRC. My first test I had an IGG 31 IND. Third test I had IGM 30+ along with IGG 31 IND. That is the only time band 30 has shown for me. Igenex suggested epitope tests for the 31's and the 30. All negative.
Chuck - your group 2 concerns me. One could have Bart and Lyme - and due to having Bart - be considered Lyme neg - because of cross-reacting bands?
ie some bands could be Bart and Lyme -so if in doubt - call it non-specific - which could result in overall result as neg. for LD? That's real logical. That is the best anyone can do with these tests?
Another reason I guess we hear "it's a clinical diagnosis" so often - because many folks have co-infections.
quote: A second group of 45 serum samples (Group 2) consisted of samples positive for other tick-borne infections, including ten positive for Babesia , two positive for Bartonella , and 33 positive for Anaplasma or Ehrlichia . Of these samples, 12 were provided with accompanying patient histories by the proficiency survey agencies, and 33 were collected from specimens submitted to the authors' laboratory for testing for tick-borne diseases in 2005. The latter samples were positive by IFA for non-B. burgdorferi tick-borne diseases with titers equal to or greater than 1:160. Specimens were not included if the proficiency survey agency stated that the sample was from a patient diagnosed with Lyme disease. For patient sera received for testing in-house, only those that were negative for B. burgdorferi antibodies were included in the evaluation.
ALL Western Blot lyme bands (Bb immunogenic proteins), and there are over 100 known - I have a list to which I have added their kDa's and some GenBank info - are subject to false positives and false negatives. It is true for IgM and it is true for IgG. And that which can generate a false positive on a Western Blot probably will increase the likelyhood of a false positive on an ELISA. The ELISA is an integrative (integral calculus-like) test.
I just found a list of false positive by band that I have yet to digest completely but it includes bands I wasn't aware were known to have false positives.
BLASTP (Wikipedia is your friend) is used to find potential cross reactive bands. I have used it. Interpreting the results is the difficulty. For me, at least.
"it's a clinical diagnosis" because testing is far from perfect.
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