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» LymeNet Flash » Questions and Discussion » Medical Questions » Dr. Alan MacDonald's Autopsy Findings on a 19 Month Old

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Author Topic: Dr. Alan MacDonald's Autopsy Findings on a 19 Month Old
WakeUp
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Please donate to Alan's Research at:

http://tinyurl.com/oqwuonq

His latest findings after autopsy of a 19 month old child who died of Lyme:


1)Infection: simultaneous presence of:Burgdorferi Borrelia,in Myocardium and in Autopsy Brain tissue sections(loosegroupings or single Borrelia microbes devoid of extracellularMatrix material.

2.Heavy load of infectomes as granular and cylindrical, spiral units: Infectious burden isQualitatively estimated by comparing the density of Borrelia in60x microscopic fieldsIn patient tissue –

with the density of laboratory cultures of pure B31 strain Borrelia burgdorferiGrown in vitro in BSK culture medium –in late log phase of Bacterial GrowthCurve

3.Biofilm Communities in Tissue (i.e. Communities of borrelia with Extracellular matrix present –Biofilms are identified in this patient’s Autopsy tissues -as detailed above in select tissueSites. Biofilms of microbes in human tissue are by definition, ALWAYS a marker of Chronic Infection.

Large diameter biofilms (matureforms) and small diameter biofilms (early biofilm communities) are present in the tissues submitted. Large diameter biofilms, with prominent Water Channel spaces, are evidence for persistent infection.

Biofilms BEGIN ASsmall diameter communities of membrane bound DNA/MRna ofliving granular borrelia in ExtracellularDNA containing Matrix.Water channel spaces are not yet present in the early biofilm Communities.DNA, MRna, PROTEINS in the Extracellular Matrix of biofilms are derived


..... read more and see amazing pictures at link

http://tinyurl.com/qdsflhy

..................................................

(breaking this up for easier reading for many here)

[ 11-09-2015, 04:21 PM: Message edited by: Robin123 ]

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WakeUp
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Dr. MacDonald--

WE LOVE AND APPRECIATE YOU!!

Posts: 696 | From New York | Registered: Aug 2006  |  IP: Logged | Report this post to a Moderator
WakeUp
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Even $5 would help...!!

WE WIL FIND A CURE!

http://tinyurl.com/oqwuonq

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Marnie
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"Dr. Nelson emphasized that for Lyme disease diagnosis, the CDC recommends a 2-step process of serologic testing:

first, an FDA-cleared enzyme immunoassay,

followed in immunoassay-positive or equivocal cases

by confirmatory Western blot.

***Only patients positive on both tests are considered to have Lyme disease.***"


http://webcache.googleusercontent.com/search?q=cache:Y_qKr4FCHrwJ:http://www.medscape.com/viewarticle/823840

To make *healthy* antibodies, adequate Mg and Ca are needed.

Our antibody to OspB wasn't "right", but Mg restored it:

"A confocal microscopy study was undertaken to characterize the bactericidal effects of

the Fab fragments of CB2, an immunoglobulin G1 murine monoclonal antibody,

to an epitope in the carboxy region of the outer

surface protein B (OspB) of Borrelia burgdorferi.

The bactericidal effect of Fab-CB2 is not dependent on the induction

of spirochetal proteases but

is dependent on the presence of Ca2

and Mg2 .

Supplementation of Ca2 - and Mg2 -free medium with these cations *restored* the bactericidal effects of Fab-CB2. The mechanism by which

a Fab fragment of an antibody destroys a bacterium directly may represent a novel form of antibody-organism interaction."

http://tinyurl.com/oovda2h

CB2...in ALS, MS, AD, et al also.

Cannabinoid receptor.

https://en.wikipedia.org/wiki/Cannabinoid_receptor_type_2

It is IgG1k specifically. I linked a long time ago.

Ofatumumab (HuMax-CD20; Arzerra, GlaxoSmithKline/Genmab) is a recently approved

fully human type I

***anti-CD20***

IgG1k mAb

http://webcache.googleusercontent.com/search?q=cache:bg-bCy-psooJ:http://www.mjhid.org/index.php/mjhid/article/view/2013.027/602

2013 - Yea for the Italians!

Since Mg levels PLUMMET at the outset of lyme, it is vital to restore levels as best as possible prior to testing, IMO.

Why in DOGS do doctors (vets) know the following:

"High levels of antibodies are believed to indicate active infection.

The problem is how to interpret negative or low level positive results.

A negative result is generally interpreted to mean the dog is not infected,

although it could also mean the dog's body simply has mounted no immune defense against the infecting organism.

Low levels of antibodies may indicate a poor immune response against an active infection, or past exposure to the organism but no active infection."

http://tinyurl.com/o9d9rv8

Repeating very old info. for newbies:

A ``novel form of antibody-organism interaction?'' I don't THINK so!

E. Required by immunological process. Magnesium, immunity, and allergy: Mg is required for several steps of immunological reactions

1. Lymphoblastic transformation,

a prerequisite of secretion of antibodies by lymphoblasts,

requires Ca2+ and Mg2+


2. Mg is required for synthesis of proteins, immunoglobulins included


3. Antibody-induced complement activation is Mg dependent


4. The antigen-immunoglobulin-complement reaction induces degranulation of the mastocyte

http://www.mdschoice.com/elements/elements/major_minerals/magnesium.htm

Given the fact that Mg levels absolutely DIVE at the outset of lyme, it is critical to get the levels back up before WB testing.

[ 11-08-2015, 10:23 AM: Message edited by: Marnie ]

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LisaK
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marnie, I so wish I could read all this and get it... I used to be able to do that. stupid lyme brain now. I am so glada that lyme doesn't kill everyone's brains.

yes, God bless Dr M.

gives me some hope that maybe one day my brain can be more normal

--------------------
Be thankful in all things- even difficult times and sickness and trials - because there is something GOOD to be seen

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WakeUp
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Marnie,

God bless you for continuing to have faith in organizations like the CDC and their innacurate diagnostic tests--- and outright lies about Lyme disease, Morgellons and Ebola..

Officials there have been caught in so many lies about Lyme, Morgellons and Ebola---- that I (and increasing numbers of other people) don't believe a thing that comes out of that corrupt organization anymore. ( Fool me once, shame on you... fool me twice shame on me, as the saying goes...)

Sad and terrifying, to know that our tax dollars are being spent by liars who do not have our best interests at heart---- but true if you will dig into the unreported story. ( Yes, the media is owned by the same small group that controls the CDC at the top).

They don't like us very much.

Thanks for the info anyway...

I wish I were wrong... [Frown]

Peace...

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WakeUp
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Sorry Marnie--

Could you possibly summarize your finding above (outside of the CDC info of course...lol)

Im trying to figure out what FAB CB-2 is(a monoclonal antibody--- ie a manufactured antibody?) , and how it relates to the cannabinoid receptor--- I will definitely read more, but if you can summarize it in two sentences for dunces , I would be grateful.

Also, a question-- since borrelia practices antigenic variation and cloaks itself in our own fibrin and in goo, and hides inside biofilm, how would this expensive monoclonal antibody work in these cases?

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WakeUp
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Hi Marnie--

I just read the article--- will read it again--- very interesting.. Thank you for posting.

I was also thinking about the fact that OSpA is super antigen, is it not? Meaning that any vaccine using synthetic OspA causes arthritis-- even when no actual borrelia is present... No?

I believe this is why the Lyme vaccine was taken off the market... although Paul Mead bald faced lied about it and said that Lymerix was taken off the market due to "lack of demand..." LOL

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WakeUp
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One last thing Marnie,
So correct me if Im wrong--- in order for this FAB monoclonal antibody to work properly in killing spirochetes(above)--- it requires Ca2 and Mg2-- are these minerals produced naturally in the body from regular Ca and Mg?

And does this FAB monoclonal antibody work against biofilm and cysts?

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WakeUp
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BUMP FOR ALAN

Please donate to Alan's Research at:

http://tinyurl.com/oqwuonq

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Marnie
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IgG1k (thanks to the Italians!)= a *very* specific antibody.

the "K" = anti-inflammatory.

***"anti-human CD20 IgG1k mAb"

"B-lymphocyte antigen CD20"

It appears the antibody - IgG1k - binds to CD20 (antigen = protein) on B lymphocytes which may stop them from working.

"OspB ***inhibits the phagocytosis*** and

oxidative burst of human neutrophils

whereas OspA induces the oxidative

burst in neutrophils."

"Induce *memory* B cell immune responses."

http://webcache.googleusercontent.com/search?q=cache:BzmgMGobEhgJ:http://journal.frontiersin.org/article/10.3389/fimmu.2014.00310/pdf

"B lymphocytes are the cells of the immune system that make antibodies to invading pathogens like viruses. They form memory cells that remember the same pathogen for faster antibody production in future infections." Wikipedia.

GREAT easy read 2015 link:

http://webcache.googleusercontent.com/search?q=cache:_flfoSTWlmwJ:http://news.ucdavis.edu/search/news_detail.lasso%3Fid%3D11254


B cells make our antibodies. Our antibody to OspB looks like it may have been not appropriately made due to our 1st reaction to OspA which may have impacted our kidneys -> Mg levels plummeted.

Antibodies take several days to be made.

Besides:

"These results strongly suggest that BIP (B cell inhibitory protein ***in the tick's saliva***) may facilitate B. burgdorferi transmission by

preventing B-cell activation,

and also highlights the potential of BIP as a therapeutic agent in B-cell maladies."

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1782588/

More than one protein in the tick's saliva look to impact our response negatively - to Bb's benefit.


CD20-CD20L ("L = ligand = binder)are proteins which bind together like Velcro in MANY "autoimmune diseases".

Our T cells "talk to" our B cells. It looks like the "conversation" was not interpreted correctly.

Which is WHY antibiotics are necessary. Our own ANTIBODIES don't work to clear Bb and they, if made, are made too late or clear out of the system.


Mg is anti-inflammatory AND anti-histamine AND inhibits HMG CoA reductase (like a statin to lower cholesterol).

Romanian doctors CURED EARLY ONSET lyme in TWO patients via restoring very depleted Mg levels AND giving IV abx.

Remember...it takes several DAYS to mount an "healthy" antibody defense.

IgA (is a first responder to Bb - OspA).

See my post today about how IgA can impact the kidneys (which control the electrolyte levels - including Ca and Mg).

Need for Omega 3 fatty acids.

"OspA, administered intranasally (i.n.) or intragastrically, induced strong serum IgG and

salivary gland IgA responses."

IgA salivary...uhm...L-theanine to counter or the omega 3 fatty acids?

Adding to the complexity...IgG1k -

Nucala (mepolizumab) is an

interleukin-5 antagonist monoclonal antibody (IgG1 kappa)

indicated for the add-on maintenance treatment of patients with *severe eosinophilic* asthma.

http://tinyurl.com/p3279cv

These results indicate that the selective

proliferation of

IgM rheumatoid factor-secreting B-1a cells is

induced by co-stimulation by the specific pathogen antigen

***and IL-5…***

http://www.ncbi.nlm.nih.gov/pubmed/25452118 2015

"Of these cytokines, interleukin 5 appears to have the greatest role in the regulation of eosinophil maturation."

About eosinophils here:

https://en.wikipedia.org/wiki/Eosinophil_granulocyte

Skim granule proteins (see *eosinophil-derived neurotoxin*!!!) and Eosinophilia in the above link!


P.S. the "demand" for using BOTH tests - including the often inaccurate WB testing - to "confirm" lyme infuriates me.

And...I can't help wonder if persons with blood type O don't develop "autoimmune" and can't mount a better response to Bb?

http://www.helpmychronicpain.com/blog/bid/74613/Your-Blood-Type-and-AutoImmune-Disease-Inflammation-and-Fatigue

Wake up...L-theanine (chewed - doesn't taste bad), 200mg in the morning MIGHT help your brain fog. Get your doc's approval!

This one:

http://www.amazon.com/Natural-Factors-Stress-Relax-Suntheanine-Chewable/dp/B000OEPAH0/ref=pd_sim_121_4?ie=UTF8&dpID=41VXqxXrQXL&dpSrc=sims&preST=_AC_UL160_SR93%2C160_&refRID=0W69CV CMQNAXR7EWWP7Z

Google L-theanine and beta amyloid. It also looks to impact gamma delta T cells - positively.

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