epartment of Cardiology, Institute for Clinical and Experimental Medicine, Videňsk� 1958/9, Prague, Czech Republic. Abstract AIMS:
Recent studies in patients with dilated cardiomyopathy (DCM) have detected the genome of Borrelia burgdorferi sensu lato (BBSL) in endomyocardial biopsy (EMB) specimens using
a qualitative polymerase chain reaction (PCR), suggesting a causal link between Lyme disease and DCM in areas in which Lyme disease is endemic.
We aimed to study this relationship using a comprehensive molecular analysis detecting BBSL in EMB samples. METHODS AND RESULTS:
We performed a comprehensive histopathological, immunohistochemical, ultrastructural, and molecular analysis targeting cardiotropic viruses and BBSL in EMB specimens of
41 individuals with recent-onset DCM and 15 controls with end-stage coronary artery disease.
Specifically, quantitative PCR and electron microscopy of EMB specimens were employed.
In addition, autoantibodies and manifestation of autoimmune diseases were evaluated in both groups.
Individuals with recent-onset DCM presented more frequently with myocardial BBSL persistence as compared with the control group (24% vs. 0%, P = 0.035).
In contrast, the prevalence of parvovirus B19 and cytomegalovirus was similar in both groups.
Sequence analysis of borrelial DNA revealed the following genospecies: Borrelia burgdorferi sensu stricto in three patients (30%), Borrelia afzelii in two patients (20%), and Borrelia garinii in four patients (40%),
the results being inconclusive in one case.
BBSL-positive DCM patients had a higher prevalence of organ-specific autoimmune diseases in comparison with the remaining DCM patients (50% vs. 16%, P = 0.030). CONCLUSION:
Myocardial persistence of BBSL may be involved in the pathophysiology of DCM in individuals living in areas in which Lyme disease is endemic. ------------------- Listen close cos' they are telling PCR is not the bomb....
-------------------- Suspected Lyme 07 Test neg One band migrating in IgG region unable to identify.Igenex Jan.09IFA titer 1:40 IND IgM neg pos 31 +++ 34 IND 39 IND 41 IND 83-93 + DX:Neuroborreliosis Posts: 5850 | From Kentucky | Registered: Dec 2008
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