Tincup
Honored Contributor (10K+ posts)
Member # 5829
posted
The NY University School of Medicine said... "Skin tattooing [as a vaccine delivery method] is a novel technique that is safe, cost-effective, and convenient."
Researchers in the Netherlands said... "To conclude, ... DNA tattoo is a promising vaccination strategy against spirochetes."
Sample 'DNA Tattoos' are at the link at the bottom of the page. Might want to pick which tattoo you're going to want, and where you want it.
J Vis Exp. 2012 Oct 18;(68). pii: 50032. doi: 10.3791/50032.
Skin tattooing as a novel approach for DNA vaccine delivery.
Chiu YN1, Sampson JM, Jiang X, Zolla-Pazner SB, Kong XP. Author information 1Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, USA.
Abstract Nucleic acid-based vaccination is a topic of growing interest, especially plasmid DNA (pDNA) encoding immunologically important antigens. After the engineered pDNA is administered to the vaccines, it is transcribed and translated into immunogen proteins that can elicit responses from the immune system.
Many ways of delivering DNA vaccines have been investigated; however each delivery route has its own advantages and pitfalls.
Skin tattooing is a novel technique that is safe, cost-effective, and convenient. In addition, the punctures inflicted by the needle could also serve as a potent adjuvant.
Here, we a) demonstrate the intradermal delivery of plasmid DNA encoding enhanced green fluorescent protein (pCX-EGFP) in a mouse model using a tattooing device and b) confirm the effective expression of EGFP in the skin cells using confocal microscopy.
PMID: 23117298 [PubMed - indexed for MEDLINE] Share on FacebookShare on TwitterShare on Google+
AND....
Gene Ther. 2014 Oct 2. doi: 10.1038/gt.2014.87. [Epub ahead of print]
Rapid outer-surface protein C DNA tattoo vaccination protects against Borrelia afzelii infection.
Wagemakers A1, Mason LM1, Oei A2, de Wever B2, van der Poll T3, Bins AD4, Hovius JW5. Author information
1Department of Internal Medicine, Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. 2Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands. 31] Department of Internal Medicine, Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands [2] Division of Infectious Diseases, Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands. 4Division of Medical Oncology, The Netherlands Cancer Institute-Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands. 51] Department of Internal Medicine, Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands [2] Division of Infectious Diseases, Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands [3] Amsterdam Multidisciplinary Lyme Center, Academic Medical Center, Amsterdam, The Netherlands.
Abstract
Borrelia afzelii is the predominant Borrelia species causing Lyme borreliosis in Europe. Currently there is no human vaccine againstLyme borreliosis, and most research focuses on recombinant protein vaccines against Borrelia burgdorferi sensu stricto. DNA tattooing is a novel vaccination method that can be applied in a rapid vaccination schedule.
We vaccinated C3H/HeN mice with B. afzelii strain PKo OspC (outer-surface protein C) using a codon-optimized DNA vaccine tattoo and compared this with recombinant protein vaccination in a 0-2-4 week vaccination schedule.
We also assessed protection by DNA tattoo in a 0-3-6 day schedule. DNA tattoo and recombinant OspC vaccination induced comparable total IgG responses, with a lower IgG1/IgG2a ratio after DNA tattoo.
Two weeks after syringe-challenge with 5 × 105 B. afzelii spirochetes most vaccinated mice had negative B. afzelii tissue DNA loads and all were culture negative. Furthermore, DNA tattoo vaccination in a 0-3-6 day regimen also resulted in negative Borrelia loads and cultures after challenge.
To conclude, DNA vaccination by tattoo was fully protective against B. afzelii challenge in mice in a rapid vaccination protocol, and induces a favorable humoral immunity compared to recombinant protein vaccination.
Rapid DNA tattoo is a promising vaccination strategy against spirochetes.Gene Therapy advance online publication, 2 October 2014; doi:10.1038/gt.2014.87.
PMID: 25273355 [PubMed - as supplied by publisher] Share on FacebookShare on TwitterShare on Google+
Keebler
Honored Contributor (25K+ posts)
Member # 12673
posted
- Oh, no . . . no . . . no . . . no. For so many reasons: no.
They are trying to lure with "sexy" approaches. The dyes used for the "tattoo" are not likely going to be from blueberries but from industrial chemicals. -
[ 10-04-2014, 02:36 AM: Message edited by: Keebler ]
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Keebler
Honored Contributor (25K+ posts)
Member # 12673
posted
- I will say that the "patch" approach for some vaccines may (I hope) better than some of the serums offerings that contains some terrible additives.
TED Talks had a very good presentation about this about 6 months ago. I'll see if I can find that tomorrow.
But, for lyme, the vaccine is lacking in the depth of understanding all about lyme / TBD. But that's another topic, another brain I would need to adequately remark.
That they want to call it a "tattoo" is very telling. It's all in the marketing of it. -
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Keebler
Honored Contributor (25K+ posts)
Member # 12673
posted
- Not about lyme, at all.
No matter what you think about any vaccine, this is a remarkable presentation and could (possibly?) help in many ways to eliminate the additives, a huge problem -- although that was not exactly the initial goal here.
Demo: A needle-free vaccine patch that's safer and way cheaper
TED Global 2013
Mark Kendall - Video of 13:50 in length - June 2013 -
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beaches
Frequent Contributor (1K+ posts)
Member # 38251
posted
Just popping in to say that moms don't have a vote in their kids' tattooing. If we did, the number of tats would be slim to none, none or just one being preferable.
And no, I didn't read the article or links. Just responding to the tattoo thing.
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