Medical Microbiology Borrelia Spirochetes in human blood: fluorescence in situ hybridization – FISH Alan B MacDonald* *Corresponding author: Alan B MacDonald Department of Research, The Dr Paul H Duray MD Research Endowment Trust, Inc, Naples, FL, USA F1000Posters 2015, 6: 110 (poster) [English] Poster [815.69 KB] Add to Twitter Add to Mendeley http://cdn.f1000.com/posters/docs/302473778
Not presented at a conference Background / Purpose: Demonstration of the efficacy of fluorescence in situ DNA hybridization (FISH Method) for the diagnosis of chronic Borreliosis infections in the human host.
Main conclusion:
FISH DNA hybridization using molecular beacon DNA probes specific for Borrelia burgdorferi provides evidence for the diagnosis of Borrelia burgdorferi infection in human hosts whose conventional antibody (serology testing) produces negative or inconclusive results.
FISH method testing produces images of the Borrelia DNA-containing Spirochetes by direct imaging. This technology enables the correct diagnosis of Borrelia infections which are ambiguous or negative for antibody production by the host in serology testing.
Disclosures: No relevant competing interests disclosed. COMMENTS (2) add a comment 12 Feb 2015 | 2:26 PM Eva Sapi The strength of this poster to show that molecular beacons could be very powerful tools to find Borrelia especially in bodily fluids. There is also a very important point that beacon probes are capable demonstrating all the forms of Borrelia not just the spirochete forms therefore they provide additional information for the clinicians. The next step would be a double blinded clinical study to see if the probe can be used for Lyme disease diagnosis.
Eva Sapi PhD DISCLOSURES The author has no competing interest 11 Feb 2015 | 11:05 PM Alan MacDonald University of West Haven
ADDENDUM : VALIDATION STUDIES COMPLETED TO RATIFY THE SPECIFICITY OF MOLECULAR BEACON DNA PROBES IN THIS PRESENTATION.
Validation is required for purposes of Quality Control when a new laboratory instrument, testing protocol, or laboratory reagent becomes available for use in hospital practice in laboratories which are operated by Board-Certified Anatomic and Clinical Pathologists.
UN-validated instruments, protocols, or reagents are withheld from established and approved entities in the diagnosis of patient disease. The Molecular Beacon DNA Probes utilized in this Presentation were VALIDATED FOR THEIR ABILITY TO HYBRIDIZE WITH THE DNA OF BORRELIA BURGDORFERI SS and to FAIL TO HYBRIDIZE WITH THE DNA OF ANY OTHER LIFE FORM ON PLANET EARTH.
Therefore, fluorescent signals emanating from Spirochetes in the "Controls" panels ( green FITC fluorescein molecule) indicate that the DNA of burgdorferi-type Borrelia was contained inside of the green fluorescent structure, and further, that the green fluorescent images are spirochetes of the Borrelia Burgdorferi species.
SUBMITTED. 11 th day of February, year. 2015, Alan B. MacDonald ,MD, Fellow, College of American Pathologists DISCLOSURES Disclosures / Competing Interests : None.
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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lpkayak
Honored Contributor (10K+ posts)
Member # 5230
posted
Two of my heros...alan and eva
-------------------- Lyme? Its complicated. Educate yourself. Posts: 13712 | From new england | Registered: Feb 2004
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posted
Sweet, between this, Claritin, and more research maybe we will get some where. I know someone is just going to come along and shoot down my hope....Don't be a negative Nancy...
-------------------- Lyme flare June, July, August of 2013. Diagnosed September 2014 Lyme, Bartonella, Mycoplasma, Mono Posts: 595 | From Texas Crossroads | Registered: Oct 2014
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posted
The Beacon Probe test is even better than the excellent culture test because it can be done immediately - no need to wait months for a result.
Yes, this test can get us everywhere, but only if we ourselves mobilise politically to fight for it. We know the Denialists will do everything they can to try to discredit the test. I would not be surprised if they now launch an almighty campaign to discredit Dr MacDonald.
Beacon Probes are the most advanced technology for detecting the DNA of an organism.
It's shocking too that DNA of Borrelia burgdorferi and Borrelia miyamotoi (relapsing fever bacteria) are present IN THE SAME BUG.
With this hybrid genome, it could well produce miyamotoi proteins instead of burgdorferi ones. Miyamotoi is undetectable on a Lyme elisa or western blot!
Elena
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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posted
This comment I see has now been added by Dr Mayne, who explains why Beacon Probes are superior to PCR and even to the previous generation of DNA probes (linear probes).:
"Peter Mayne Ilads
FISH methods are now a preferred method for the serial measurements of the chronic myelogenous leukaemias BCR/ABL2 and related family of mutations In malignant clonal populations of bone marrow leukemia cells. This is because the exact DNA sequences of the ABL site may deviate from subtypes ABL1 , ABL2 , etc. PCR only succeeds if the correct primer nucleotides are present to bind to the BCR/ABL mutation site. ABL1 sequences differ from ABL2 sequences.
Therefore a failure of PCR may be encountered if primers for ABL1 are used in a patient whose mutation is ABL2. Ideally FISH DNA probes target regions of DNA in the pathogen or in the nucleotide sequence of a region of clinical concern. If the FISH probes are designed to target DNA regions which are not subject to mutation, then molecular beacons (circularized SsDNA) confer added precision when compared to linear DNA probes.
Beacons require 100% match in potential target DNA in order for MB probes to hybridise. Linear DNA probes may hybridise to potential DNA or mRNA sites if there is less than 100% sequence match with the nucleotides of the linear probe. Beacons can boast that even a single base mismatch will completely prevent Beacon Probe hybridization with potential Target DNA in a mixed population of nucleotides. (see www.molecular-beacons.com )
The exact sequence of the FlaB probe, and your success with using this probe to prove the existence of borrelia as pathogenic in the paper that you published using the beacons FlB is proof of pathogenesis. "
Elena
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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posted
I am very disappointed that the F1000 site has censored a paragraph from my comment there, which highlighted the fact that Dr MacDonald has found Borrelial DNA in the tissues of Alzheimer's Disease victims using the Molecular Beacon DNA Probe method.
This is really vital information. There are tens of millions of Alzheimer's sufferers in the world and the numbers are set to skyrocket. If no cure is found, the numbers will continue to climb, causing a health and economic crisis of huge proportions.
F1000 state th following: "F1000 does not screen, edit, publish or review Comments prior to their appearance on the website and Comments do not reflect the views or opinions of F1000, its agents or affiliates. "
So I really don't understand why the material on Alzheimer's was removed from my comment.
Please do your best to circulate the news about this excellent Molecular Beacon DNA probe method which is confirming Lyme disease in seronegative chronic patients, including those who have died and could, perhaps, have been saved if they had had treatment.
Elena
Elena
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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posted
While trying to tell others on Facebook about the fact that my paragrpah on the Alzheimer's-Lyme connection was removed from my F1000 post, I had the information removed in real-time from my post on Facebook (!)
I am having constant problems now accessing Facebook and security software throws up alerts suggesting my account/computer is being tampered with.
It's not just a computer issue as my phone communications are also being tampered with.
I have had this before many times and have had a computer expert testify in court about the harassment. But I believe it's specially intense at the moment because I am circulating news about the Molecular Beacon DNA probes for Borrelia finding it in Alzheimer's brain tissue, as well as the fact that a chimeric (mixed-DNA) borrelia with a hybrid-burgdorferi/miyamotoi genome has been found.
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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LisaK
Frequent Contributor (1K+ posts)
Member # 41384
posted
anyone have a link to simple laguage that tells what to do about lyme alzhiemers. I really think I have this and I can't read very well any more. I need simple info on what steps to take to help this.
????
-------------------- Be thankful in all things- even difficult times and sickness and trials - because there is something GOOD to be seen Posts: 3558 | From Eastern USA | Registered: Jul 2013
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Rumigirl
Frequent Contributor (1K+ posts)
Member # 15091
posted
Oh, Lord have mercy, Eight (about your surveillance and the immediate removal of part of your posts!!! Very, very alarming.
Posts: 3771 | From around | Registered: Mar 2008
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I think the first step (if you havent done so already) would be to try and get some confirmation that you have Lyme by using a better testing method than the two-tier method which is extremely bad.
You could try the Advanced Lab culture test, or if you cannot afford that, the immunoblot and/or PCR offered by Igenex.
If these are pos and/or you have a clinical diagnosis from an LLMD, then you might well find that the same abx protocol that helps with other Lyme symptoms will help with memory and other cognitive symptoms.
Rumigirl, there has been a deliberate coverup re the Borreliosis-Alzheimer link for decades, so it's not surprising if those responsible are interfering with the communications of those of us who want the truth known. It may well be that the F1000 staff had ntohing to do with the censorship, or were leaned on.
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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MADDOG
Frequent Contributor (1K+ posts)
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posted
Why does the websight not work for me???
MADDOG
Posts: 3997 | From Ohio | Registered: Oct 2000
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droid1226
Frequent Contributor (1K+ posts)
Member # 34930
posted
What did Alan McDonald do to get well again. At the end of Under Our Skin, it said he developed alzheimer's and his research went unfinished. Then he got well again. What is the deal there?
posted
An update - I owe a huge apology to the staff at F1000 - they did not censor my comment at all. It appears someone else got into the account/website and deleted all reference to Alzheimers from my comment.
I think this is a very sad state of affairs.
The f1000 editorial staff have been very helpful and restored my account (which eventually had been blocked too) and enabled me to comment again.
At the time of writing, my comment is there still. However a year ago I and others commenting to the medical portal Medscape about the Alzheimer-borrelia link had our accounts and comments tampered with.
Here's my comment, currently up there as of 24 March 2015 14:21 GMT. Elena ----------- Borrelia Spirochetes in human blood: fluoresence in situ hybridization – FISH _Borrelia Spirochetes_ in Alan B MacDonald* *Corresponding author: Alan B MacDonald Department of Research, The Dr Paul H Duray MD Research Endowment Trust, Inc, Naples, FL, USA F1000Posters 2015, 6: 110 (poster) [English] Poster [815.69 KB] Add to Twitter Add to Mendeley
Not presented at a conference Background / Purpose: Demonstration of the efficacy of fluorescence in situ DNA hybridization (FISH Method) for the diagnosis of chronic Borreliosis infections in the human host. Main conclusion: FISH DNA hybridization using molecular beacon DNA probes specific for Borrelia burgdofreri provides evidence for the diagnosis of Borrelia burgdorferi infection in human hosts whose conventional antibody (serology testing) produces negative or inconclusive results. FISH method testing, produces images of the Borrelia DNA containing Spirochetes by direct imaging. This technology enables the correct diagnosis of Borrelia infections which are ambiguous or negative for antibody production by the host in serology testing. Disclosures: No relevant competing interests disclosed. COMMENTS (6) add a comment 24 Mar 2015 | 2:08 PM Report Elena Cook personal capacity My comment for some reason became corrupted and my main point truncated. I was trying to underline the enormous significance of the fact that these Molecular Beacon DNA probes have found evidence of spirochaete bacteria in autopsy Alzheimer's disease brain tissue. The spirochaete in question is Borrelia. This genera of bacteria causes Lyme disease and Relapsing fever.
Recently the team of Blanc et al in Strasbourg found that a number of their dementia patients with serological evidence of Borrelia burgdorferi in blood and CSF began to improve, and/or saw an arrest in the progress of their dementia, after treatment with IV ceftriaxone. With these powerful Beacon probes, many patients whose borrelia infection may be missed by the insensitive serology tests in use could potentially be detected. The opportunities for prevention, roll-back or even cure of Alzheimers suggest that this finding is too important to be ignored.
I'd like to thank the staff at F1000 for restoring my apparently corrupted account.
Elena Cook DISCLOSURES None declared.
-------------------- Justice will be ours. Posts: 786 | From UK | Registered: Oct 2007
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