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» LymeNet Flash » Questions and Discussion » Medical Questions » Ticks in Texas - GenBank and Sequencing Errors? Sound familiar?

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Author Topic: Ticks in Texas - GenBank and Sequencing Errors? Sound familiar?
susank
Frequent Contributor (1K+ posts)
Member # 22150

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Article in this week's Houston Chronicle:
(Scroll down to the end and read under insects)

http://www.houstonchronicle.com/sports/article/Risks-are-as-real-as-rewards-in-outdoor-recreation-6362048.php

(45% of deer ticks collected/tested in south Texas carry Bb)

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I thought to research this further and found:

http://retractionwatch.com/2014/11/05/lyme-disease-researchers-call-for-retraction-of-paper-on-deer-ticks

(only 1% carry Bb)
(read the links also)
-----------
Remembering a report from Texas A&M from a few years ago found this:

http://www.researchednutritionals.com/information.cfm?ID=275

(25% of ticks collected/tests carry Bb)
(the original article was in the Houston Chronicle and other publications).

So which is it? 1% 25% 45% ???

Problems with sequencing. Sounds familiar to me as in Advanced Laboratory Services culture test vs the CDC. (I had a positive culture from specimen sent in end of 2011).

--------------------
Pos.Bb culture 2012
Labcorp - no bands ever
Igenex - Neg. 4 times
With overall bands:
IGM 18,28,41,66 IND: 23-25,34,39
IGG 41,58 IND: 39
Bart H IGG 40

Posts: 1613 | From Texas | Registered: Aug 2009  |  IP: Logged | Report this post to a Moderator
Tincup
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Sorry! Can't see article unless subscribed.

--------------------
www.TreatTheBite.com
www.DrJonesKids.org
www.MarylandLyme.org
www.LymeDoc.org

Posts: 20353 | From The Moon | Registered: Jun 2004  |  IP: Logged | Report this post to a Moderator
susank
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I corrected the link a few times. Still a no-go?
Tutu - do you have a way to post the article from FB? (computer dumb dumb here).

--------------------
Pos.Bb culture 2012
Labcorp - no bands ever
Igenex - Neg. 4 times
With overall bands:
IGM 18,28,41,66 IND: 23-25,34,39
IGG 41,58 IND: 39
Bart H IGG 40

Posts: 1613 | From Texas | Registered: Aug 2009  |  IP: Logged | Report this post to a Moderator
susank
Frequent Contributor (1K+ posts)
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The Houston Chronicle article basically says be careful as 45% of deer ticks in S. Texas have tested positive for the agent that causes Lyme Disease. Symptoms listed.

I thought 45% sounded frighteningly (word?) high - so did a quick search and found the restraction website.

That one is the "must read".

Also did not know there was also a dispute over the 25% reported by Texas A&M from a few years ago. I thought that number was accepted.

But there are naysayers - of course.
What is the truth?

--------------------
Pos.Bb culture 2012
Labcorp - no bands ever
Igenex - Neg. 4 times
With overall bands:
IGM 18,28,41,66 IND: 23-25,34,39
IGG 41,58 IND: 39
Bart H IGG 40

Posts: 1613 | From Texas | Registered: Aug 2009  |  IP: Logged | Report this post to a Moderator
SacredHeart
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I got lyme in Arlington. I would assume it is probably true that we are in, or moving towards a serious public health crisis here.

--------------------
Lyme flare June, July, August of 2013. Diagnosed September 2014 Lyme, Bartonella, Mycoplasma, Mono

Posts: 595 | From Texas Crossroads | Registered: Oct 2014  |  IP: Logged | Report this post to a Moderator
poppy
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Calling for a retraction is not the same as a retraction.

The other side routinely does this in order to cast doubt on results they don't like, as in the ones that make people realize lyme is a real threat, etc.

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PatTxLyme
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At least one party who made the request for retraction has an unreported conflict of interest. It is possible that the research finding 45% of Texas Ixodes scapularis ticks to be infected with Lyme contradicts the positive rate found by the diagnostic test on which his patent is based. The request also speculates that the TAMU lab was contaminated with Bb, but the strain he mentions was not introduced to the lab. The research at TAMU on the ticks was carried out with the highest possible standards and the article went through many revisions and tests before being accepted by Parasites & Vectors. It mirrors the empirical evidence of patients. Other recent research has found a large discrepancy between cases of Lyme (based on reported cases)in Texas vs. predictions of cases based on probable tick habitat. Reasons for the discrepancy were not given, but one speculation was that the tests were not picking up all cases. We know they can miss up to 60%, so this is not surprising. Those of us in the Texas Lyme community welcome the TAMU research for demonstrating that Lyme is a bigger problem here than the reported cases (based on ELISA followed by Western Blot)suggest.

--------------------
Txlyme moderator

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Lymetoo
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PatTxLyme .. It would be helpful to break up your post so everyone can read it. Thanks!

Susan .. I haven't seen any of this on Facebook. You can bring the link here from FB IF the article has a URL.

--------------------
--Lymetutu--
Opinions, not medical advice!

Posts: 96223 | From Texas | Registered: Feb 2001  |  IP: Logged | Report this post to a Moderator
Lymetoo
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PS . . My LLMD told me many years ago that approximately 85% of ticks in the North East were infected with Lyme and more.

--------------------
--Lymetutu--
Opinions, not medical advice!

Posts: 96223 | From Texas | Registered: Feb 2001  |  IP: Logged | Report this post to a Moderator
susank
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Tutu - will see what I can do.

The TAMU info (25%) and the retraction letter (45%) is what is the most interesting.

This so much reminds me of the culture test vs CDC. Contamination? Sequencing?
Eerily similar.

Tired.......sorry grammar/spelling....

--------------------
Pos.Bb culture 2012
Labcorp - no bands ever
Igenex - Neg. 4 times
With overall bands:
IGM 18,28,41,66 IND: 23-25,34,39
IGG 41,58 IND: 39
Bart H IGG 40

Posts: 1613 | From Texas | Registered: Aug 2009  |  IP: Logged | Report this post to a Moderator
susank
Frequent Contributor (1K+ posts)
Member # 22150

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Searching and ended up on LymenetEurope.

This very subject was being discussed there March of this year.

Very interesting. Worth reading under "Science".

It's been a long time since I read anything on LNE - had thought it had shut down. Guess not.

The usual folks are still there - if you know what I mean.

--------------------
Pos.Bb culture 2012
Labcorp - no bands ever
Igenex - Neg. 4 times
With overall bands:
IGM 18,28,41,66 IND: 23-25,34,39
IGG 41,58 IND: 39
Bart H IGG 40

Posts: 1613 | From Texas | Registered: Aug 2009  |  IP: Logged | Report this post to a Moderator
Lymetoo
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quote:
Originally posted by susank:

The usual folks are still there - if you know what I mean.

-
No thanks! [Big Grin]

--------------------
--Lymetutu--
Opinions, not medical advice!

Posts: 96223 | From Texas | Registered: Feb 2001  |  IP: Logged | Report this post to a Moderator
   

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