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» LymeNet Flash » Questions and Discussion » Medical Questions » Pleomorphic Borrelia study

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Author Topic: Pleomorphic Borrelia study
Brussels
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If you aren't still convinced about Borrelia pleomorphism, read this very detailed 12 page study.

Microbiology research Pleomorphic forms of Borrelia burgdorferi

1) Borrelia - like many other bacteria - are able to differentiate in many different pleomorphic forms in different situations: when attacked by antibiotics, for example, or when temperature drops or raises, when they are starved...

2) there is not simply 'cell wall deficient forms' and 'cell wall forms'. In fact, there is NO cell wall deficient form (according to this research).

All pleomorphic forms have cell walls!!

3) Round Bodies (usually called L-forms or cysts) do have cell walls, but they are formed by different proteins than normal 'adult' Bb spirochete.

Round bodies are also called L-forms, protoplasts, spheroplasts, cysts... It's all the same thing!


4) The authors think there is MORE AND MORE plausible evidence that pleomorphism help the bacteria to EVADE the IMMUNE SYSTEM and DECREASE ANTIBIOTIC SUSCEPTIBILITY.


5) Each pleomorphic form of Borrelia burgdorferi has unique biochemical signatures, meaning, they are very probably doing DIFFERENT activities / functions in the host (us).

Different symptoms?


6) the researchers divided pleomorphic Borrelia forms in groups of 4:

A: spirochaete (the super-star: long cork shaped)

B: spirochaete with membrane bleb (kind of not so pretty anymore...)

C: Round body = spherical, pretty, but not 'naked' as previously thought

D: called BFL = colony with at least 10 spirochaetes, blebs and RB (round bodies), all together for party time. they are fairly common!

Look at the photos on Fig 1, page 519 to get the picture in your mind!


7) The coolest photos are in Fig 4: you actually SEE transformation from spirochaete to Round form!!


8) Efflux pump for toxic compounds = that is the way bacteria cleans (pushes out) toxic compounds such as antibiotics, so that it does not die or get damaged.

The Efflux pump B. burgd utilizes is the RND transporter system, and that is how antibiotic resistance occurs (in case of Bb).

This efflux pump works even in Round Bodies!!! So the still active L-form / cyst is still able to pump out antibiotics or toxic compounds!!


8) we usually hear that Round bodies (L-forms, cysts) do not move, but look at Fig 6: they do have flagella (little feet!!!).

They still use ATP, but in lower amounts, so they are motile!!


9) How do they culture all these pleomorphic forms? They say it is very easy to do:

...b y AGGRESSING the spirochaete, providing unfavorable conditions for it!

But they say that even if conditions are good, there will be pleomorphic forms (but in less amounts).


10) More environmental stress = more formation of 'blebs', which are sort of 'protrusions' on spirochaete that will form pleomorphic forms such as Round Bodies.

Look at what they say: Blebs (protusions on the bacterial cell) contain TIGHTLY PACKED DNA and maybe involved in transfer of genetic material.

Posts: 6199 | From Brussels | Registered: Oct 2007  |  IP: Logged | Report this post to a Moderator
Brussels
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If bacteria are not intelligent, what are they then?
Posts: 6199 | From Brussels | Registered: Oct 2007  |  IP: Logged | Report this post to a Moderator
TF
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Pre-programmed (by a very intelligent intelligence/creator) to survive.

Intelligence requires a pretty high-level brain. If a living thing has a high-level brain, you should be able to locate it and examine it.

We see animals acting "intelligently" in so many cases--eagles making nests, mating, sitting on their eggs at great personal cost, handling the eggs properly, handling the chicks properly, teaching them how to fly, catch fish, and everything else they need to survive, etc.

We call it instinct. It means pre-programming. It is all pre-programmed into the DNA by some great intelligence I call God.

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Brussels
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According to Buhner, they have neuro-like-structures when acting in a group, so a 'brain'!!

Buhner adds: bacteria communicate with other species, act in synergy with them, wait for better moments to launch an attack, etc.

I guess it's more than instinct, in this case! [Smile]

Posts: 6199 | From Brussels | Registered: Oct 2007  |  IP: Logged | Report this post to a Moderator
TF
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Lots of animals communicate with others. I don't see why that can't be instinct.

I have never studied instinct, but I have read that ants communicate to others in the ant colony. Bees stand at the entrance to the bee hive and do a dance that communicates where they found pollen, type of pollen it is, etc.

DNA is like file cabinet after file cabinet of very detailed information arranged in meticulous order. DNA is unbelievable. It controls everything, so why cannot DNA include instructions to the organism on how to communicate with other organisms, act together with them, etc.?

Living organisms sense the environment around them, including what other organisms are doing. We see this when an animal flees from us once we get too close for their comfort.

I saw a cat come in my yard and a rabbit stand stone still the entire time. Good idea! The cat never saw the rabbit. After about 10 minutes of looking, the cat left. The rabbit waited another 5 minutes, then ran away as fast as possible.

I thought that rabbit was pretty smart. But, maybe it does all this by instinct.

Really, without being an expert on DNA, all we can do is marvel at what we see in nature. But, although it may appear as intelligence to us, it could easily be pre-programming.

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Brussels
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TF, nice examples, thanks!!

I also think nature is much smarter than we are taught!!

It's just that the word pre-programmed involves pre-knowledge... without LEARNING, if you see what I mean.

Antibiotics are very recent in history, and bacteria hadn't had contact with that for millions of years. Only now, the last speck of time for them, they are having contact with various man-invented drugs.

And they do REACT, make different efflux-pumps, borrow GENES from other species that already have resistance, then teach them to future generations through their genes.

It's just the word 'pre-programmed' that does not fit to our actual situation, meaning, man made antibiotics and how bacteria still seem to be THINKING, trying, borrowing genes, transferring these to their species, other interspecies, and other totally different species!¨

In my opinion, there is no pre-programing in that because our drugs are new to them, if you see what I mean... [Wink]

I agree that 'survival at any costs' is instinct, and that INTENTION and WISH TO SURVIVE are pre-programmed in all species, including ourselves.

That is why we are fighting so much to get our health back! Same way Bb and other bacteria try to fight that much to continue living!

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TF
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Antibiotics came from nature. Penicillin was produced by a fungus. Streptomycin, chloramphenicol, and tetracycline are produced by soil bacteria.

See The Natural History of Antibiotics here:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2731226/

The first time the word "antibiotic" was used as a noun, it meant "any small molecule made by a microbe that antagonizes the growth of other microbes."

So, antibiotics have always been found in nature. Man has just learned about this and then used it to "make" more or better antibiotics.

Also, pre-programming means without learning. But, the one that pre-programmed everything into the DNA could easily pre-program for things that would exist in the future also. It all depends on the abilities you believe the pre-programmer has.

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Brussels
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According to buhner, man cannot make better remedies than nature.

Nature had millions of years to evolve and produce DIFFERENT chemicals to CANCEL and ENHANCE the effect of single chemical molecules.

It's a wrong idea that isolating ONE chemical will do BETTER than hundreds or even THOUSANDS of chemicals that are present in ONE single plant.

It's a bit like saying that eating a meal of vitamin pills is BETTER than eating a meal with fruits and vegetables!

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Lymedin2010
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You can watch my video here, where I studied one species of tick spirochete & noted many of the pleomorpohic forms in the video. Part 2 of this video will be coming out this month, with more lessons & interesting finds.

Also, for the first time ever I show you how the granular forms or blebs form into juvenile spirochetes in a time lapse video toward the end.

https://www.youtube.com/watch?v=7OaPWZBW36Q

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Brussels
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Lymedin, thank you for posting that video!

It looks really cool!

How did you get so many ticks? And still, how do you extract the juice from their guts?!!

And a probably very dumb question of mine: how do you know all those stuff are Borrelia and not the hundreds of other pathogens that ticks carry?

If these bacteria are really all Borrelia, Buhner is more than right to call that not a single organism, but a SWARM.

Bacteria are amazing beings, and their non-stop 'creative' process amazes me!!

Posts: 6199 | From Brussels | Registered: Oct 2007  |  IP: Logged | Report this post to a Moderator
Lymedin2010
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I had someone collect them for me, the same way you see them collected in the UOS videos w/felt cloth dragged through grass & brushes.


In the info of my first tick video, I give you the exact tick juice squashing procedure I use. Keep in mind this is for nymph ticks & they are small & more manageable. With adult ticks I may choose to do it the way Willy B. did, with mortar & pestle and grinding them and then taking a small sample to the glass slide.
https://www.youtube.com/watch?v=RnFR4Ca5MVo


Ticks can be kept alive for months in a small vial w/some moisture (wet napkin), but they must be in a cool place or otherwise they die really quickly in the heat & warmer weather.


I PCR tested those spiros for the major Borrelias & reveal the results in the video & so I think they might be a different type. Could even be reptilian spiros, who knows exactly and it does not matter either as the lessons are repeated in this spiro with the various morphologies & biofilm.


I had been observing them for months & by now I can tell what is what in tick juice. All the other pathogens, although they might be there don't seem to survive & perhaps die quickly when I add water to the sample. And so nothing else grows or shows up besides various spiros & what look like filarial nematodes.


Here is one of my tick videos:
https://www.youtube.com/watch?v=ZTBVsHA8Svg


Here are 2 more filarial videos & it looks like the spiros are attracted to the outer surface of might be filarial worms.
https://www.youtube.com/watch?v=hif23sJNzy0&t=2s

https://www.youtube.com/watch?v=U79-y4IXQvQ&t=9s

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Brussels
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They look very cool! Whatever these worms are, they are HUGE compared to Bb.

I'm supposing you are enjoying watching your blood more than using it as measure of getting better or testing treatments?


Funny thing is that we keep hearing Bb is so tiny compared to huge pathogens such as Babesia.

Maybe you need to stain them to see them?

It is anyway very cool to actually see Bb changing forms, on and on again, then probably inside and around these nematodes, which really makes us think that treatment should be done aiming bigger pathogens too, specially worms.

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Lymedin2010
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At first it was for monitoring & discovering, but now I have lost hope with such a long time treating & getting nowhere.

Now I do it for the pure joy of discovery & passing the information on so that others can investigate it further if they see fit. We have learned a great many things as a community in the Lyme microscopy world.

You don't need to stain to see them, but some of us have gone on to do stains to be able to see the co-infections, those you need to stain for. Live staining will be the next big leap forward.

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Brussels
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You guys are amazing!!!

When you learn the staining part, please open a small business and let us send you our samples for you to watch!! [Smile]

Have you given up? You sounded pretty energetic still, in your videos, so I suppose your lyme is not that bad, right?

Thank you for sharing the info, the amazing images, your thoughts on each of the wriggling stuff you found swimming! It is a fascinating world.

I used energy testing to tune my treatments. I guess, when we reach about 80% of functionality, it is VERY hard to find what will help us, because everything we tried before had not worked.

That is when, I think, energy testing becomes a very valuable tool.

It can pin point very specific areas you haven't seen, ...

...or find the most crazy herbs or treatments,

... or THE acupuncture points you never treated,

...or THE psycho blockades you haven't even thought were important

... or points in your body that are ill and that affect the whole body (like teeth, scars, accidents, points on the spine...).

Anyway, thank you for sharing.
Very nice videos! Keep us posted of your new discoveries!!

Posts: 6199 | From Brussels | Registered: Oct 2007  |  IP: Logged | Report this post to a Moderator
   

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