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» LymeNet Flash » Questions and Discussion » Medical Questions » Microscope advise needed

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Author Topic: Microscope advise needed
ojr1979
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Member # 52123

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Hi all new on here so please go easy on me.Hopefully im posting in the right place. I have been suffering with health issues the last 14 years or more and could never work it out and neither could the Drs. Ive been tested for for all sorts including Lyme which came back negative but as we all know the test used is pointless for atleast half of us. I do plan on getting testing done after this lockdown is over with by sending my blood to Arminlabs in Germany. However i have recently bought a microscope to look for Lyme etc ......

https://www.ebay.co.uk/itm/SWIFT-Professional-Research-Grade-2500X-LED-Lab-Trinocular-Compound-Microscope/372959728632?ssPageName=STRK%3AMEBIDX%3AIT&_trksid=p2060353.m2749.l2649

I have bought the slides,cover slips and immersion oil and used the methods set out by Borrellia Tamer on youtube. I can see hundreds of black/white dots moving erratically around in the blood samples. Heres some vids.....

https://www.youtube.com/watch?v=xqRrsPgTUdg

https://www.youtube.com/watch?v=Fm7RICBVC-c

So my question to everyone on here is what can i do to make these easier to see or make out. Would anyone like to guess what they are? Im sure this has been asked before but i am desperate to get to the bottom of this. Many thanks all

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lymenotlite
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The Microscopy Thread

http://flash.lymenet.org/ubb/ultimatebb.php/topic/1/120458#000000

Posts: 678 | From WA state | Registered: Jul 2011  |  IP: Logged | Report this post to a Moderator
ojr1979
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Could an administrator delete this thread as ive re published this in the microscopy thread. Thanks Lymenotlite
Posts: 7 | From UK | Registered: May 2020  |  IP: Logged | Report this post to a Moderator
Garz
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I haven't read the microscopy thread - I may add that to my reading list but wanted to say well done for getting this far in terms of getting reasonable images from a microscope on a budget.

it's not easy. I went down this track a little way looking for gut parasites as I had lots of gut symptoms and negative lab tests. but then some MD's say that several negative tests mean there is no parasite as often the tests will not pick it up - and so I reasoned that I could buy a microscope and do it myself every day for weeks for the price of 1 or two tests.

one of the issues you quickly run into is that of various artifacts that look like something sinister - but may not be

for instance, anything really small suspended in liquid will tend to move around randomly and or vibrate due to something called Brownian motion - basically stuff getting bumped into by water molecules that are vibrating - as all molecules in liquids above their melting points vibrate and move around a lot.

i'm guessing the microscopy thread will go into this in more detail - but there is an issue with trying to spot boreliia with a light microscope - even the best ones.
this is basically due to the very small diameter of the borrelia spirochete - its basically too thin vs the wavelength of visible light - and as a result, it simply doesn't show up ( is effectively invisible ) in standard techniques.
the normal technique for visualizing them in blood etc is to use a technique called dark-field microscopy = direct light passing through the target is blocked and instead a special condenser arrangement is used to bounce light off the target at acute angles - enabling the very thin spirochete to be seen due to refraction type effects
eg here https://www.youtube.com/watch?v=DnsuiSKGDRs

note how everything in suspension jiggles in that clip also

I suspect the moving particles in the last clip you posted may also be an artifact - possibly some kind of lensing effect by the round structure/cell causing a kind of enhanced focus on a few particles in direct line with it.

you could consider staining as a possible route to explore more potential pathogens. many can be stained with things like Giemsa - and that can be done at home.
certainly babesia and bartonella also - although for the very small bacteria like that you need a very good quality microscope ( up to 400 or co can fit in a singe red bloocd cell.

also bear in mind if you are still alive after 14 years then the levels of bacteremia or parasitemia ( the number of infected cells vs normal ones ) is v unlikely to be as shown in text books - those are usually the most extreme cases.
in reality, in many diseases the levels typically found are one pathogen per entire field of view at 400 or 1000x - esp if it's a chronic condition.

I share your pain and commend your ingenuity and persistence

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