Clin Microbiol Infect. 2011 Dec 7. doi: 10.1111/j.1469-0691.2011.03749.x. [Epub ahead of print] High frequency of false positive IgM immunoblots for Borrelia burgdorferi in Clinical Practice. Seriburi V, Ndukwe N, Chang Z, Cox ME,
Wormser GP. Source
Division of Infectious Diseases, New York Medical College, Valhalla, NY, USA. Abstract
Clin Microbiol Infect ABSTRACT:
Although it is known that two-tier serologic testing for Lyme disease may be associated with false positive results on the IgM immunoblot, this problem has never been systematically studied in the clinical practice setting.
In a retrospective investigation of patients referred to the private adult practice of an Infectious Diseases physician for possible for Lyme disease,
50 of 182 patients (27.5%, 95% CI: 21.1-34.6) were found to have a false positive IgM immunoblot.
78.0% of these patients had received unnecessary antibiotic therapy.
False positive results were not restricted to any single commercial laboratory.
Research on alternative testing strategies that eliminate the IgM immunoblot entirely is warranted.
� 2011 The Authors. Clinical Microbiology and Infection � 2011 European Society of Clinical Microbiology and Infectious Diseases.
PMID: 22369185 [PubMed - as supplied by publisher]
-------------------- Suspected Lyme 07 Test neg One band migrating in IgG region unable to identify.Igenex Jan.09IFA titer 1:40 IND IgM neg pos 31 +++ 34 IND 39 IND 41 IND 83-93 + DX:Neuroborreliosis Posts: 5850 | From Kentucky | Registered: Dec 2008
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posted
Hi Pinelady- Do you know if they used some type of PCR test to determine which patient "did not" have Bb infection? I can't figure out how one would be able to read the entire study.
Posts: 65 | From oregon | Registered: Jun 2011
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AuntyLynn
Frequent Contributor (1K+ posts)
Member # 35938
posted
EXACTLY hammond!
HOW on EARTH did they CLAIM to get a FALSE POSITIVE??? With the friggin' ELISA?? LOL!!!
I was screaming about this on another Lyme site yesterday. These guys must have some pretty good PERKS to protect if they feel the need to run a study like this one at ALL! (Follow the $$$ was exactly what came to my mind!)
Can anyone open the FULL STUDY? I'm dying to know what tests they CLAIM to have gotten this "false positive."
Does anyone else smell a rat?
Posts: 1432 | From New Jersey | Registered: Jan 2012
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poppy
Frequent Contributor (1K+ posts)
Member # 5355
posted
A very big rat!
The question is why this kind of crap gets published. So much for peer review. Or maybe all the peers are equally corrupt.
Posts: 2888 | From USA | Registered: Mar 2004
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posted
We now have a potentially powerful tool to shut up the IDSA. They have dishonestly gotten their old 2006 medical guidelines renewed for 5 years. Five more years without insurance reimbursement for us and no research for a cure!!
We need to make a huge fuss about this. We need to let the National Guidelines Clearinghouse, the government agency that gives legitimacy to these IDSA guidelines, that they need to be revised by involving all stakeholders (that includes us). We need to make their dishonestly as public as possible . PLEASE sign the petition at www.lymedisease.org and send it to everyone you know.
If we stay focussed on getting many, many thousand signatures we will show the Clearinghouse that they are being watched as to whether they hold the IDSA accountable.
It is the one action that we now have available that can stop IDSA now. Ellen
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Posts: 819 | From New York, NY | Registered: Oct 2001
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posted
BINGO!! The IDSA panel guys sit on the panels of journals. Medical research and guidelines are very corrupted ever since they became lucrative because of corporate money buying research (and researchers).
We are face to face with an old boy's network, fueled by corporate money that has co-opted medical research. The IDSA is adept at using this system.
But right now we are much more endangered by them if they succeed in getting away with dishonestly renewing their 2006 guidelies. If we don't stop them, we are stuck for 5 more years with the "standard of care" saying that there's no such thing as chronic Lyme disease.
So I'm begging. Please get as many signatures as possible on the petition by Lorraine Johnson and Lymedisease.org (formerly CAlDA) at www.lymedisease.org Ellen
quote:Originally posted by poppy: A very big rat!
The question is why this kind of crap gets published. So much for peer review. Or maybe all the peers are equally corrupt.
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Posts: 819 | From New York, NY | Registered: Oct 2001
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TABLE 1. Criteria used to evaluate whether the IgM immunoblot was likely to be false-positive 1 Failure to meet CDC criteria for seropositivity - 94%a A First-tier test omitted - 40%a B First-tier test negative - 22%a C Symptoms in excess of 4 weeks with a negative IgG immunoblot - 90%a D Immunoblot did not meet CDC band criteria for reactivity - 12%a 2. Lack of tick exposure - 42%a A. Testing done in winter months of December, January, February or March - 38%a B. No exposure to geographic area where the vector ticks are known to be present - 10%a 3. Symptoms atypical for early localized or early disseminated Lyme disease, i.e., no erythema migrans-like skin lesion, no acute febrile illness, no meningitis, no cranial nerve palsy, no radiculopathy, and no evidence for myocarditis; or asymptomatic - 98%a 4. Lack of seropositivity by repeat serologic testing within 4 weeks of the positive IgM immunoblot - 40%a
CDC, Centers for Disease Control and Prevention.
aIndicates percentage of the 50 patients regarded as having a false positive IgM immunoblot for antibodies to B. burgdorferi who fulfilled this criterion. Of the four categories of criteria listed, all patients fulfilled at least two of them, 58% fulfilled at least three, and 16% fulfilled all four.
Posts: 300 | From Northeast | Registered: Dec 2006
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posted
Allie, where did you find this info? I 'm guessing CDC website but I can't find it with regards to this study. This is really pathetic if this is how they came up with their false positive numbers. No EM rash is evidence of a false positive?
Posts: 65 | From oregon | Registered: Jun 2011
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posted
I have access to the publication. I copied and pasted word for word their table 1, entitled "Criteria used to evaluate whether the IgM immunobolot was likely to be false-positive."
I hope I don't get pulled over by the copyright police for doing that?
Since it is only a small portion of the paper, I think it's ok.
Rightyo, getting the test in the winter was grounds for false positive. yuppers.
Allie
Posts: 300 | From Northeast | Registered: Dec 2006
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posted
This study is a total CROCK. They're FISHING for false positives, when the real issue is false NEGATIVES.
There is no way to PROVE someone is a false positive. I can guarantee you if all those "false positives" were cultured under Advanced Lab's new test, most if not all would be culture positive for Borrelia.
BTW, EXCELLENT article in today's Washington Post. How gratifying to see the TRUTH about Lyme Disease in a major newspaper.
Read it and add your comment (have to register first).
-------------------- Sick since at least age 6, now 67. Decades of misdiagnosis. Numerous arthritic, neuro, psych, vision, cardiac symptoms. Been treating for 7 years, incl 8 mos on IV. Bart was missed so now treating that. Posts: 765 | From nw ct | Registered: Sep 2008
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-------------------- Sick since at least age 6, now 67. Decades of misdiagnosis. Numerous arthritic, neuro, psych, vision, cardiac symptoms. Been treating for 7 years, incl 8 mos on IV. Bart was missed so now treating that. Posts: 765 | From nw ct | Registered: Sep 2008
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quote:Originally posted by ellenluba: But right now we are much more endangered by them if they succeed in getting away with dishonestly renewing their 2006 guidelies. If we don't stop them, we are stuck for 5 more years with the "standard of care" saying that there's no such thing as chronic Lyme disease.
So I'm begging. Please get as many signatures as possible on the petition by Lorraine Johnson and Lymedisease.org (formerly CAlDA) at www.lymedisease.org Ellen
[/QB][/QUOTE]
Yes, everyone PLEASE sign the petition. Also email all your friends and family asking them to sign. I did this and got about a dozen more signatures.
Tip: Tell your friends/family, "Please sign. It will only take a minute, and it will mean a lot to me."
-Paulie
-------------------- Sick since at least age 6, now 67. Decades of misdiagnosis. Numerous arthritic, neuro, psych, vision, cardiac symptoms. Been treating for 7 years, incl 8 mos on IV. Bart was missed so now treating that. Posts: 765 | From nw ct | Registered: Sep 2008
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posted
Allie- Thanks for the info. What a joke. Funny how if you don't pass the CDC seropositive threshold, your geographic area never becomes positive either. So the current Lyme Map that the CDC creates is probably a very narrow gene pool of Bb. There are +150 strains of Bb and the CDC creates it's own little world of Bb's.
And if you ARE seropositive but live in a "non-CDC map area" it meets a criteria for false positive in this test!??!?? I don't know how they crunched their numbers in this study but winter time testing is a criteria?!? Wow.
Great article in the Washington post. Thanx Paulieinct!
Posts: 65 | From oregon | Registered: Jun 2011
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Pinelady
Frequent Contributor (5K+ posts)
Member # 18524
TprC/D (Tp0117/131), a trimeric, pore-forming rare outer membrane protein of Treponema pallidum, has a bipartite domain structure.
Anand A, Luthra A, Dunham-Ems S, Caimano MJ, Karanian C, Ledoyt M, Cruz AR, Salazar JC, Radolf JD. Source
Departments of Medicine. Abstract Identification of Treponema pallidum (Tp) rare outer membrane proteins (OMPs) has been a longstanding objective of syphilis researchers.
We recently developed a consensus computational framework that employs a battery of cellular localization and topological prediction tools to generate ranked clusters of candidate rare OMPs (Cox DL et al., Infect. Immun. 78: 5178-5194).
TP0117/TP0131 (TprC/D), a member of the Tp repeat (Tpr) family, was a highly-ranked candidate.
Circular dichroism, heat-modifiability by SDS-PAGE, Triton X-114 phase-partitioning and liposome incorporation confirmed that full-length, recombinant TprC (TprC(Fl)) forms a β-barrel capable of integrating into lipid bilayers.
Moreover, TprC(Fl) increased efflux of terbium-dipicolinic acid complex from large unilamellar vesicles and migrated as a trimer by Blue-Native PAGE.
We found that in Tp, TprC is heat-modifiable, trimeric, expressed in low abundance, and, based on proteinase K accessibility and opsonophaocytosis assays, surface-exposed. +++++++++++++++++++++++++++++++++++++++++++ From these collective data, we conclude that TprC is a bona fide
rare OMP as well as a functional ortholog of E. coli OmpF. +++++++++++++++++++++++++++++++++++++++++++ We also discovered that TprC has a bipartite
architecture consisting of a soluble N-terminal portion (TprC(N)), ================================ presumably periplasmic and bound directly or indirectly to peptidoglycan, ================================ and a C-terminal β-barrel (TprC(C)). ================================== Syphilitic rabbits generate antibodies exclusively against TprC(C),
while secondary syphilis patients fail to mount a detectable antibody response against either domain. +++++++++++++++++++++++++++++++++++++++++++++++ The syphilis spirochete appears to have resolved a fundamental dilemma arising from its extracellular lifestyle, namely,
how to enhance OM permeability without increasing its vulnerability to the antibody-mediated defenses of its natural human host. ----------------------------------- They have discovered the cause of AIDS/MadCow/Lyme It is prion like in its abilities to hide. AIDS/MadCow was a hoax----HIV never killed anyone. Which means all syndromes can be treated to cure by killing all the infections in hiding and kicking the immune system back on to fight.
http://www.youtube.com/watch?v=HhAUC0M9JgI Dr. Judy Mikovits summarizes at the NIH Workshop 4/8/11--------listen to what she says---1983 was when they first used genetic equivalent for ecoli in vaccine for HepB vaccine for gay men.
These peptides contain an N-palmitoyl group at the N-terminal residue, which is a modified cysteine,
containing a S-[2,3-bis(acyloxy)-(2-R,S)-propyl] moiety.
When this residue (Pam3Cys) is at the N-terminus of a synthetic peptide,
it acts as potent immunoadjuvant to enhance both IgM and IgG antibody responses to the attached peptide. -------------------------------------- Conventional analytical procedures (e.g., Edman degradation and amino acid analysis)
are either not applicable due to the N-terminal modification,
or do not provide confirmation of the intact structure. ------------------------------------- Chromatographic analysis is also hindered by the tendency of these lipoidal Pam3Cys peptides to -------------------------------- form large aggregates, and in some cases to be permanently adsorbed on reversed phase columns. -------------------------------- We have applied several mass spectrometric techniques, including fast atom bombardment (FAB), electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) to characterize the intact structures of a number of different Pam3Cys synthetic peptides. The MALDI-MS has been found to be the most sensitive for the analysis of the structure of Pam3Cys peptides. 0000000000000000000000000000 They got an immune responce and then like majik it disappeared, but it really went no where because what happens is it swaps its genes with other infectious organisms and us to hide them all. They gave us the GOD like protein in vaccines. The only difference is the immune system they are in.
http://www.youtube.com/watch?v=DIHy4_31Db4&feature=youtu.be Listen how the HepB test found 88% of cases of HIV. You will see the arrogance here with what the NIH did to Don Francis as is much like they do to all our LLMD's because of criminals.
We got some honorable researchers out there yet. http://www.ncbi.nlm.nih.gov/pubmed/22269175 Despite extensive histological and serological evaluation a definitive diagnosis was not established initially.
Thirteen months after initial presentation, hepatic bartonellosis was diagnosed by PCR studies from surgically excised liver tissue.
But you guys gotta get well first, so you can be on the front line, when the crooks fall.
-------------------- Suspected Lyme 07 Test neg One band migrating in IgG region unable to identify.Igenex Jan.09IFA titer 1:40 IND IgM neg pos 31 +++ 34 IND 39 IND 41 IND 83-93 + DX:Neuroborreliosis Posts: 5850 | From Kentucky | Registered: Dec 2008
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posted
Thanks Paulie for reinforcing my request that people sign the petition. The subject of this thread has to do with "can't we shut them up." Well, here's the best opportunity we've ever had to do so. Please sign at www.lymedisease.org
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Posts: 819 | From New York, NY | Registered: Oct 2001
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