LymeNet Flash: What causes Bb to develop biofilms?

This is topic What causes Bb to develop biofilms? in forum Medical Questions at LymeNet Flash.

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Posted by Marnie (Member # 773) on :

I'm gonna get to the bottom line first..how's that for a change? ;-)

"Serine acetyltransferase is a key enzyme in the

sulfur assimilation

pathway of bacteria and plants, and is known to form a bienzyme complex with O-acetylserine sulfhydrylase, the last enzyme in the cysteine biosynthetic pathway."

Bb has replaced all Fe-S enzymes with those that use Mn. Bb does not use Fe or S.

If the gene for serine acetyltransferase (cysE) is NOT present = biofilm.

How did I get there?

I followed this path:

http://ethesis.helsinki.fi/julkaisut/laa/haart/vk/alitalo/compleme.pdf

Go to 8.11 when linked above.

Additional:

***Extracellular secretion of the Borrelia burgdorferi Oms28 porin*** and Bgp, a glycosaminoglycan binding protein

Proteomic liquid chromatography-tandem mass spectrometry analysis of tryptic fragments derived from strain B31 culture supernatants confirmed the identity of the 28-kDa species as Oms28 and revealed a 26-kDa protein as 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs-2), previously described as Bgp, a glycosaminoglycan-binding protein.

The ***release of*** Oms28 into the culture medium is more selective when the spirochetes are in logarithmic phase of growth compared to organisms obtained from stationary phase.

As determined by immunoblotting, stationary-phase spirochetes

released OspA, OspB, and flagellin.

Oms28 secreted by strains B31, HB19, and N40 was also recovered by radioimmunoprecipitation.

This is the first report of B. burgdorferi *** protein secretion*** into the extracellular environment.''

http://www.garfield.library.upenn.edu/histcomp/bassler-bl_citing/node/894.html

My note: It is believed it is only our response to flagellin which is long lasting....as we try to break it down.

Definition: The outer membrane-spanning (Oms) proteins

http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=178274&blobtype=pdf

Above link only if you want to get REALLY into taking apart that protein.

``These exoproteins may be important in helping the spirochete to survive within the host, evade the immune system, and directly or indirectly cause pathology.

Several exoprotein genes have been cloned and recombinant protein will be used to test hypotheses that these factors play a role in the host-parasite interaction.

Oms28, a previously described pore-producing protein, may damage host cells.

Enolase, which is an enzyme involved in glucose metabolism and related to the same enzyme in streptococci that triggers protein degradation, may serve as a spreading factor following mammalian infection.''

http://www.middlebury.edu/academics/ump/majors/chem/hours/rcluss.htm

E. Coli produces the same...Oms28!!!

Next sentence is KEY!!!

``Loss of cysE ***enhances biofilm formation*** in

Escherichia coli''

Okay...what is cysE?

``The CysE enzyme,

*serine acetyltransferase*,

catalyzes the conversion of serine to O-acetyl-L-serine (OAS). This activating signal was also produced by Escherichia coli, and

production was abolished in a strain containing a null allele of cysE.''

http://www.garfield.library.upenn.edu/histcomp/bassler-bl_citing/node/919.html

Although SAT (serine acetyltransferase) of Escherichia coli, which catalyses the first step in cysteine synthesis."

Serine acetyltransferase is a key enzyme in the

***sulfur assimilation pathway***

of bacteria and plants,

and is known to form a bienzyme complex with O-acetylserine sulfhydrylase, the last enzyme in the cysteine biosynthetic pathway."

No need for sulfur = biofilm. No cysE gene(serine acetyltransferase)= biofilm.

Fe can destroy Bb...sulfur too?

Logically Bb does NOT want serine, esp. phosphatidylserine which rebalances the HPA axis.

Our most dangerous pathogens are those that use Mn.

Posted by hiker53 (Member # 6046) on :

Layman's translation for your wonderful research, please.

Posted by SForsgren (Member # 7686) on :

You mentioned Mn - Manganese. Can you comment further on how these bugs use Manganese? Manganese is a key in assimilation of bone and avoidance of osteoporosis which seems to be a problem that many of us develop over time.

Posted by Angelica (Member # 15601) on :

Yes please translate.

Posted by Lymeorsomething (Member # 16359) on :

There's that pesky phosphatidylserine again. I hope that supp. helps me [Smile]

Posted by Lymeorsomething (Member # 16359) on :

And what if we supp Mn? Does this throw gas on the fire or does this help at all?

Posted by Lymeorsomething (Member # 16359) on :

up

Posted by herxuk (Member # 15873) on :

From my understanding, it's Magnesium, calcium, boron, vit D, vit K and Phosphorus, needed to prevent osteoporosis. Bb likes Manganese, it seems to be what Marnie is saying.

Posted by Keebler (Member # 12673) on :

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Just tell me what I'm supposed to do. I just can't think anymore -

(wonder if Ingrid Bergman had a little lyme brain going on in "Casa Blanca" - " . . . I can't think anymore . . . ").

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I have read that allicin will help dissolve Bb biofilm but allicin is far to speedy for me.

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Posted by herxuk (Member # 15873) on :

Marnie says above, "" Our most dangerous Pathogens are those that use Manganese "" As Manganese seems to be in a many number of foods, then it could be hard to avoid. To my understanding, not good news.
[confused]