What bands do you believe are lyme specific bands? I have read alot of articles out there but I am still confused. Your thoughts appreciated.
Posted by seekhelp (Member # 15067) on :
18, 23-25, 31, 34, 39 and 83-93
Posted by ChuckG (Member # 19093) on :
quote: IGeneX Lyme Western Blots Jyotsna S. Shah
...When compared only to uninfected controls (Group 3), bands 18, 41, and 58 kDa were also highly significant, suggesting that these bands are associated with many tick-borne infections, but not in other patients.
...We have demonstrated that IgG antibodies to the 18, 41, and 58 kDa proteins detected by WB are statistically associated with tick-borne diseases.
Dump 18 . Non-specific. At least for IGeneX IgG.
Posted by Lymetoo (Member # 743) on :
Read Dr C's Western Blot Explanation. Each band is explained.
Thanks all
Posted by northstar (Member # 7911) on :
(an oldie, but the kda's are listed)
Yale J Biol Med. 1984 Jul-Aug;57(4):561-5.Click here to read Links The antibody response in Lyme disease. Craft JE, Grodzicki RL, Shrestha M, Fischer DK, Garc�a-Blanco M, Steere AC.
We determined the antibody response against the Ixodes dammini spirochete in Lyme disease patients by indirect immunofluorescence and an enzyme-linked immunosorbent assay (ELISA).
The specific IgM response became maximal three to six weeks after disease onset, and then declined, although titers sometimes remained elevated during later disease.
Specific IgM levels correlated directly with total serum IgM.
The specific IgG response, often delayed initially, was nearly always present during neuritis and arthritis, and frequently remained elevated after months of remission.
Although results obtained by indirect immunofluorescence and the ELISA were similar, the ELISA was more sensitive and specific.
Cross-reactive antibodies from patients with other spirochetal infections were blocked by absorption of sera with Borrelia hermsii, but titers of Lyme disease sera were also decreased.
To further characterize the specificity of the humoral immune response against the I. dammini spirochete, 35S-methionine-labeled spirochetal antigens were identified by immunoprecipitation with sera from Lyme arthritis patients.
These polypeptides had molecular weights of
62, 60, 47, 37, 22, 18, and 15 kDa,
and were not recognized by control sera.
We conclude that the ELISA, without absorption, is the best method to assay the humoral immune response in Lyme disease, and we have identified methionine-containing spirochetal polypeptides that may be important in Lyme arthritis.