Bb brings with �him� SALP15 � a protein that contains the ***sugars mannose and galactose*** as well as another protein called p8 that inhibits mannose binding lectin.
Analysis of carbohydrate structures on Salp15 demonstrated that recombinant Drosophila-
If protomyxzoa is implicated in MS he suggests we should be cautious about some supplements,
one is particular may be detrimental to the eradication of the organism because
*** it is a major component of the biofilm, and that is MAGNESIUM.***
It seems to make people feel better for a little while but it hampers any other medical intervention that might be tried as it helps build the defence that this organism has against the immune system and drugs.
Dr Fry explains: � So in the short term, when you give magnesium to these biofilm communities you hide them even more from the immune system,so you get a relief that is less inflammation.
But in the long term you are building up slugging, more biofilm, and enriching that community, which makes it harder and harder to get rid of.�
A biofilm is an aggregate of microorganisms in which cells adhere to each other on a surface.
These adherent cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS).
Biofilm EPS, which is also referred to as slime
(although not everything described as slime is a biofilm),
is a polymeric conglomeration generally composed of extracellular DNA, proteins, and polysaccharides.
EPS are***mostly*** composed of polysaccharides and proteins,***
but include other macro-molecules such as DNA, lipids and humic substances.
EPS are the construction material of bacterial settlements and either remain attached to the cell's outer surface, or are secreted into its growth medium
Microorganisms synthesize a wide spectrum of multifunctional polysaccharides including intracellular polysaccharides, structural polysaccharides and extracellular polysaccharides or exopolysaccharides (EPS).
Exopolysaccharides generally consist of ***monosaccharides and some non-carbohydrate substituents*** (such as acetate, pyruvate, succinate, and phosphate).
Chitin's properties as a flexible and strong material make it favorable as
surgical thread.
Posted by AuntyLynn (Member # 35938) on :
Heavy.
So Marnie, have I extrapolated correctly, that you don't agree that magnesium can make biofilms more difficult to attack?
Posted by Marnie (Member # 773) on :
Nowhere do I see Mg implicated in the *making of* biofilms. (Further down read about the crystals IN biofilm.)
(And many more links to Bb-Mn...specifically Mn-SOD which Bb uses to protect himself and we use to protect our own mitochondria from "oxidative damage".)
And iron (while Bb does not use Fe = iron DIRECTLY) is needed to make LuxS which is the gene/protein that Bb uses for quorum sensing (communicating with "his" buddys).
(I linked that previously.)
I don't agree that Mg ALONE is a *component of* Bb's biofilms.
Apparently Dr. F thinks so. Nor do I think "he" uses it to make "his" biofilm.
It appears MOST biofilms (according to Wikipedia) need iron and zinc. Bb may also use Mn, not Mg.
It is not logical that Bb would use Mg because it is a HMG CoA reductase INHIBITOR (like statin drugs and berberine) and it is an anti-inflammatory and anti-histamine.
Yes, Bb has has a transporter for Mg...in or
out?
Where is the logic if Romanian doctors cured lyme (2 patients, early onset) by giving them IV Mg - restoring very deficient levels - and IV abx.?
Where is the logic of giving Mg IV after photon treatment...if Mg is used to make biofilms?
Here is a common infection - problem re: the trapping of minerals IN biofilm:
crystals of *magnesium ammonium phosphate* and *calcium phosphate*
Mg is attached to OUR ATP as Mg-ATP. Bb's toxin looks to do this:
Mg-ATP -> Mg (out)-> ATP -> ADP + cAMP.
cAMP helps the cells survive...waiting for a chance to heal.
ADP + ribose = DNA repair...ours...IF a pathogen is not "stealing" ribose (5 carbon sugar) to make "his" RNA (remove oxygen)-> DNA.
So...Bb triggers an ongoing export of Mg it would appear.
Now some good news:
In fact, a laboratory study performed at Texas A&M University found ***stearic acid to inhibit the formation of biofilms.***
Several fatty acids (including magnesium stearate) in ground beef were tested. The results of these tests indicated that both medium- and long-chain fatty acids inhibit the formation of biofilms.
A single chocolate bar contains about 5,000 milligrams of stearic acid.
[ 08-03-2012, 11:47 AM: Message edited by: Marnie ]
Posted by sparkle7 (Member # 10397) on :
I thought that each organism that creates a biofilm is different. So, this new Dr. F bug may be different than other organisms...?
Just a "not so educated" guess. Over all the years I've been ill - they always pushed magnesium. It has never helped me actually feel better, though.
Posted by Lymedin2010 (Member # 34322) on :
I thought BB preferred a more acidic environment?
Stearic acid, the acIdic environment BB LOVES to hate?
Posted by Haley (Member # 22008) on :
Hmmmm. I think I actually understood some of this.
What if you are low in iron? You still need to get iron, don't you?
Do you think that one source of iron might be better than another, for example, maybe it is better to get from food as opposed to pill form.
I like the word "slime" much more charming than biofilm Posted by Marnie (Member # 773) on :
Look at it this way..
Bb is camped out in the tick's stomach. Bb is NOT making biofilms. Bb is expressing an outer surface protein called OspA.
cGMP = Cyclic di-GMP is Essential for the Survival of the Lyme Disease Spirochete in Ticks
Then along comes some blood...full of nutrients...glucose, iron (and other minerals), vitamins, amino acids...
But, keep in mind ONE NUTRIENT is low in our blood...Mg. BECAUSE most of our Mg is attached to our ATP as Mg-ATP INTRACELLULARLY.
I suspect the massive loss of Mg at the get-go (33% decline) means our liver probably took a "hit".
And Bb switches to expressing OspC on "his" way out. "He" picks up a "sugary protein" on "his" way out and a really bad protein designated as p8 which negatively impacts our immune defense.
What this all involves is this:
The cyclic dinucleotide c-di-GMP regulates lifestyle transitions in many bacteria, such as
the change from a
free motile state
to a biofilm-forming community.
***Riboswitches*** that bind this second messenger are important downstream targets in this bacterial signaling pathway.
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