LymeNet Flash: Detection of Lyme/Borrelia by focus floating microscopy in necrobiosis lipoidica

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»	LymeNet Flash » Questions and Discussion » Medical Questions » Detection of Lyme/Borrelia by focus floating microscopy in necrobiosis lipoidica

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Topic: Detection of Lyme/Borrelia by focus floating microscopy in necrobiosis lipoidica

nenet
Frequent Contributor (1K+ posts)
Member # 13174

posted

"Detection of spirochaetal microorganisms by focus floating microscopy in necrobiosis lipoidica in patients from central Europe."

Histopathology. 2008

http://www.ncbi.nlm.nih.gov/pubmed/18462358

"AIMS: Necrobiosis lipoidica (NL) is a chronic inflammatory skin disease with unknown aetiology. The aim was to determine the presence of spirochaetal microorganisms in NL.

METHODS AND RESULTS: Focus-floating microscopy (FFM) is a modified immunohistochemical technique that was developed to detect borrelial spirochaetes within tissue sections. It has proven to be more sensitive for the detection of spirochaetes than polymerase chain reaction (PCR). Fifty-six cases of NL as well as 44 negative and 33 positive controls were investigated for the presence of Borrelia within tissue specimens.

Using FFM, Borrelia could be detected in 42 cases (75.0%) and were seen significantly more often in histologically active inflammatory-rich (38/41, 92.7%) than in inflammatory-poor (4/15, 26.7%) cases of NL (P less than 0.001).

Seven cases investigated with a Borrelia-specific PCR (23s-RNA) remained negative. In contrast, FFM was positive in 30 of 33 (90.9%) positive controls of acrodermatitis chronica atrophicans and 15 of the positive controls (45.5%) were also positive with PCR, whereas no negative controls revealed any microorganisms.

CONCLUSIONS: Detection of spirochaetes in NL points to a specific involvement of B. burgdorferi or other similar strains in the development of or trigger for this disease."

PMID: 18462358 [PubMed - indexed for MEDLINE]

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Posts: 1176 | From KY | Registered: Sep 2007 | IP: Logged |

Pinelady
Frequent Contributor (5K+ posts)
Member # 18524

posted

Sounds like it could possibly be used in all Lyme cases. Good Works. Great News.

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Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008 | IP: Logged |

bettyg
Unregistered

posted

on page 2, up we go

IP: Logged |

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