This is topic Microscopy - anybody who recognize this structure ? in forum Medical Questions at LymeNet Flash.


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Posted by otlo (Member # 14621) on :
 
Hi ;

Anybody out there who are experienced with wet blood microscopy? This video shows a blood sample from a ten year old bedridden child. She has a long list of symptoms compliant with Lyme disease, and she is diagnosed with ME/CFS.
We use a phase contrast scope, 100x oilfilled objective.

I have observed activity like this a few times before. I am not at all sure that the observation can be related to the illness. I appreciate any views or opinions!

http://www.youtube.com/watch?v=xO1bep93Cq0
 
Posted by Gabrielle (Member # 5329) on :
 
Otlo,

I guess you are talking about the bubbly strange "cloud" moving around:

For what it is worth:

I had my blood examined with dark field microscopy by a naturopath and I had this stuff in my blood, too. So, according to this naturopath, this stuff is a form of candida.

He calls it C-candida (C stands for camouflaged) and I think this name is his invention. He says that this kind of candida cannot be detected by the usual tests.

He even wrote a book about it (in German): "Candidalismus". He says he finds this in more and more of his very sick patients. He has developped a treatment for it (isopathic) - which I did for 2,5 months. Then, the blood was clear of this stuff. At least the part that he showed me...

BUT: he also said that I was cured now and I didn't feel a bit better.

So, as I said: for what it is worth!

Gabrielle
 
Posted by CaliforniaLyme (Member # 7136) on :
 
I have no idea really but just looking around the internet-

It looks to me like a gallgang! What is a gallgang- I have no idea!!! They are in figure 5 on this site and I wrote the guy who has this site asking his opinion on your photo- hopefully he will answer!!!

See Figure 5 on this page:
http://science.uniserve.edu.au/pubs/callab/vol10/sandberg.html

It looks the same to me.

Aha, I investigated further and I believe gallgang is BILE. It appears to be a normal thing seen by microscope.

See C in this frame:
http://www.nature.com/labinvest/journal/v83/n2/fig_tab/3780604f2.html

See also
http://www.biochemj.org/bj/340/0445/bj3400445f07.gif

To me they look the same but I am not a histologist and have NO idea really!! I wrote this guy who knows ihstology to see if he knows but who knows if he will write back!!!

best wishes,
Sarah
 
Posted by CaliforniaLyme (Member # 7136) on :
 
This is stained & close up-
http://www.nature.com/labinvest/journal/v80/n5/fig_tab/3780073f7.html
 
Posted by treepatrol (Member # 4117) on :
 
Its a amoeba

Thats not good.
 -
Seen in your video it using its cytoplasm to help it capture food. Thats its litle fingers that shoot out it also uses that to move sometimes.

http://uk.youtube.com/watch?v=I3Jo7moaLdI

[ 12. February 2008, 12:43 PM: Message edited by: treepatrol ]
 
Posted by map1131 (Member # 2022) on :
 
Looking at my own microscopy and the blood evaluation sheet that my nautropath gave me in '03.

It looks to me like it might be: Eosinophils-are granular leukocytes with one or two oval lobes. Increased counts indicate allergies, parasites, tobacco smoking, drug and chemical toxicity, infection (esp fungal), low immunity. Vit A, C, B6, Zinc adrenal. Should be phagocytic, not moonlike, to be considered viable.

Has this been on the blood slides consistantly over a period of time? Or is this just one instance?

Pam
 
Posted by treepatrol (Member # 4117) on :
 
leukocytes attack

Human Macrophages - AID Virus

Neutrophil phagocytosis

[ 13. February 2008, 10:58 AM: Message edited by: treepatrol ]
 
Posted by CaliforniaLyme (Member # 7136) on :
 
Map- if you go to a histology site and look at eosinophils they look very different!!! Just fyi, meant nicely- here's just a grabbag of eosinophil photos-

http://images.google.com/images?hl=en&q=eosinophils&gbv=2
***************************************

An amoeba Tree- god I hope not!!
 
Posted by otlo (Member # 14621) on :
 
Thank you all for views and comments !

Map 1131 asks if these objects have been seen over some time. Well, I have seen many, many objects which I consider to be related - but usually those objects are kept within an outer 'static' frame, see http://www.youtube.com/watch?v=Rc3JeGoqHtk
After a few hours, there is usually no more activity, the object will remain static.


Feel free to comment on any of the videos, - they are all from people who are severely hit with symptoms compliant with Lyme Borreliosis.
From my point of view, the fact that activity closely relates to abx is interesting. After a few weeks on doxycline (ex) there is no visible activity in the samples.

http://www.youtube.com/user/otlos26
 
Posted by jamescase20 (Member # 14124) on :
 
I saw yeast in my blood under phase contrast at 1000x and she (natpath tech) told me it was yeast, it was not moving though...it was greenish. I dont know I didnt think yeast moved.
 


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