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» LymeNet Flash » Questions and Discussion » Medical Questions » Please help with my Labcorp results

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Author Topic: Please help with my Labcorp results
Fuel1212
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I am having a hard time figuring out these results. Any help would be appreciated. Even though it says negative, should I be concerned of Co's?

Chlamydia Ab Panel IgM
Pneumoniae IgM <1:20
Trachomatis Igm <1:20
Psittaci IgM <1:20

Bartonella
henselae IgG Neg <1:320
henselae IgM Neg <1:100
quintana IgG Neg <1:320
quintana IgM Neg <1:100

Chlamydia IgG
Pneumoniae IgG <1:64
Trachomatis IgG <1:64
Psittaci IgG <1:64

Ehrlichia Panel
chaffeensis IgG Neg <1:64
chaffeensis IgM Neg <1:20

HGE IgG Neg <1:64
HGE IgM Neg <1:20

Babesia microti IgM Neg <1:10
Babesia microti IgG Neg <1:10

Hopefully you are still with me [Smile]
what does all this mean???? W

THANKS!

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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Fuel1212
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up

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

Posts: 610 | From Lymeville | Registered: Nov 2010  |  IP: Logged | Report this post to a Moderator
timaca
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Well, it looks like you have not made antibodies to any of those pathogens, so they likely are not causing a problem for you.

To be honest if all you are positive for is band 41 on the Lyme western blot, I would not suspect Lyme as being a problem for you either.

You may want to get tested for Enteroviruses and other viruses. See the infection tab at this website: http://chronicfatigue.stanford.edu/

Best, Timaca

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Fuel1212
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So I guess I am on the wrong forum huh?

I don't have Lyme with 31,34,39,83-93 all IND? Why would they light up at all?

Also, I am wondering what the ratios are in correlation to being close to positive, or totally negative relative to a scale

Example:
Bart
Positive-----l----------------------l---Negative
1:20 1:320

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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sixgoofykids
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I would say in the presence of symptoms, having that many IND bands likely shows Lyme. What does your LLMD say? Lyme is a clinical diagnosis, tests are supportive of that diagnosis, but don't necessarily determine it.

I CAN'T BELIEVE they tested you for Chlamydia Psittaci!!!! I walked into a doctor's office, told him I was expossed by a sick bird that died and asked for the test.

He said the test was too complicated and expensive. But, in his defense, he did prescribe 3 weeks of 300 mg per day doxycycline, which is the treatment for it.

Still, I would have liked to have known ......

--------------------
sixgoofykids.blogspot.com

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sixgoofykids
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Also, all the Chlamydia panels don't show a range or whether they are positive/negative.

--------------------
sixgoofykids.blogspot.com

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ktkdommer
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Good questions and I can't help. I agree with your IND, they mean something. When was your last IgeneX done and has it ever been repeated? Was the first IGeneX test done without an antibiotic push?

You have a clinical diagnoses right? You had herxing with treatment and are better with treatment right?

I wish the titer cut offs were clear. I spent an hour trying to find out if ECP is 132, how high is that. I know 0-10 is normal. I couldn't find the answers anywhere. I'm still wondering how high and ECP score can go. My youngest was a 40, so a significant increase.

Hopefully someone with experience will post in soon.

So much we don't know but you keep on trying and asking those good questions.

--------------------
Things are never dull. After 3 fighting Lyme, 2 are in remission. Youngest is still sick, age 22. He has new diagnosed Chiari Malformation and Ehlers Danlos Syndrome.

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Fuel1212
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My igenex test was done a couple years ago without and abx push.

I am doing better with treatment and am positive for mycoplasma and hhv6.

I was told by my llmd that certain bands would not light up at all if there were no infection .

He said it was ind because my immune system is so messed up. CD 57 is at 19

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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Pinelady
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I agree with six. It looks like sneaky lyme to me.

Incidentally I had a new test from labcorp a month or so ago after a new bite and it showed nothing...

But the severe hives and intense itching despite antibiotics told me the test lied...

For 2 weeks I had to take benedryl every 3 hrs. to function and pushed more antibiotics and it went away...

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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Pinelady
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I wish they would get to the labcorp fraud like they have the Quest. A lot of things might change.

But I am not sure the public is prepared for just what we really face.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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Fuel1212
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Does anyone know how the titer's work?

Like is 1:64 closer to negative or positive or what is the odds.

There is no doubt my Igenex report says Lyme. I am just wondering about the co testing I just had from Labcorp.

Thanks for your interest!

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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Fuel1212
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up

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

Posts: 610 | From Lymeville | Registered: Nov 2010  |  IP: Logged | Report this post to a Moderator
timaca
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Fuel~ I don't see you being positive for any of the pathogens that you were tested for. All your values are less than the reference range, right?

For the pathogens I've been tested for, the doctors get suspicious if I have antibody titers that are 1:320 or higher. Some pathogens (Coxsackie B4 and Cpn) I've tested as high as the lab measures (indicated by >= 1:640 for example).

A well known doctor (at Columbia University Medical Center) does not count IND bands as positive (I know some LLMDs do). I would be careful at jumping at a Lyme diagnosis since you are positive to band 41 only (a band most people--healthy included--test positive for).

When you say you are "positive" for mycoplasma and HHV-6 on what are you basing your conclusion? Your labs? For info on HHV-6 testing see: http://www.hhv-6foundation.org/research/hhv-6-testing

My approach is test for lots of pathogens and treat for what looks most obviously wrong. If I had your test results, I'd still be looking for the answer....

Best, Timaca

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Fuel1212
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Yes, per Labcorp test results I was positive for Mycoplasma and HHV6.

I know we all have our opinions on here, but I think we should be careful with what we say to people. If I were a newbie and was in search of help, this information would discourage them and maybe even change their focus wrongfully.

I do appreciate your time and help, but to tell someone that "I would not suspect Lyme as being a problem for you either." because of a lonely positive 41 band. Without knowing my whole story, and without adequate knowledge IMO to determine a diagnosis.

Per Burrascano who I would say is someone we could listen to in the topic of Lyme.

Reference Site Reference Burrascano

"Chronic Lyme Disease ("Stage III") - Ill for one or more years - Serologic tests less reliable Treatment must be more aggressive and of longer duration"

This is due in part to the immune system not being healthy enough to produce antibodies because of chronic Lyme to glow bands completely positive

With regards to my diagnosis...

"CD-57 COUNT (Natural Killer Cells) . Low counts seen in active Lyme . Reflects degree of infection . Can be a screening test . Can be used to track treatment response . Can predict relapse"

My Cd 57 score was 30 when I started treatment and is now at 19, which my LLMD indicates as active Chronic Lyme and I think Burrascano would agree based on above.

Please no hard feelings, I just know how long it took for me to find answers. I was desperate. There is no doubt in my mind I have Lyme. We should be careful with what we say. After all as bad as Lyme can be it could lead to someones loss of important information and even death or suicide.

Your Friend Fuel

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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jackie51
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I too have had negative lyme tests. I've had it a long, long time.

It wasn't until i was tested for ehrlichia that my doctor finally treated me with abx.

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timaca
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Hi Fuel~ I certainly have no hard feelings towards you or others who feel that they have Lyme and hope you don't towards me. What I say is based on my own experience. I've had well over 20 WB blots done at a variety of labs (including Igenex) and I've tested positive to many bands. I started out believing I had Lyme and now I'm not so sure that is or was a problem for me! I do have a chronic bacterial infection...and whether that is Lyme or Cpn (where my IgG and IgA antibody titers measure as high as the lab measures) really no one can say. But, I have significant herx reactions to antibiotics (which my doctors have witnessed first hand) and antibiotics have improved my health and I am currently on doxy and rifampin to try to get rid of my bacterial infection. But is it Lyme? No one can really say. It is a possibility...but so is Cpn. Or maybe I have both.

Having said that, I was made aware of viruses and the role that they play in our health. One cannot tell from symptoms if you have a chronic viral infection or a chronic bacterial infection. So unless a bullseye rash is present, any "lyme" symptom can also be a chronic viral symptom. That is why, to some extent, a "clinical diagnosis" for Lyme is pretty meaningless. And why it is important to check for viruses too, for it is possible to sick with more than one pathogen (as you are aware...that is why you tested for co-infections).

It would be important for you to look at your positive results for HHV-6 and mycoplasma to see if those results indicate active infection or past infection. If you have an antibody titer of 1:320 or higher for HHV-6 (some think even 1:160 may be important) than it is worth checking into that. HHV-6 can give "lyme symptoms". See the reference that I posted above for HHV-6 testing. I did have HHV-6 and treating it has helped me.

Same for mycoplasma. For example, I had an antibody titer of 1:64. I called and talked with the assistant of Dr. Joel Baseman, who is an expert in mycoplasma. She told me that they begin to "maybe" suspect mycoplasma as an issue if the antibody titers are in the 200s or higher. That wasn't me, so I know mycoplasma is not an issue for me. (That antibody titer has since dropped to within the reference range.)

I wish you the best, and I hope we all get well, but I still think it is best to test for lots of pathogens and treat what looks most obviously wrong. I would tell any newbie that. If you haven't tested for Coxsackie B, other enteroviruses, EBV, etc, then consider doing that. You have nothing to lose and your health to gain. In me, since I've had antibody titers as high as the lab measures for Coxsackie B4 and Cpn we have treated those pathogens with improvements in my health. HHV-6 and EBV treatment has also helped me. So has antibiotic treatment.

Best, Timaca

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sutherngrl
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My first and only postive band was band IGM 41 and my LLMD made a clinical diagnosis based on my symptoms. I began treatment and a year later I tested CDC IGM positive for Lyme Disease.

This is a common accurance with lyme patients. Many test negative to begin with and later their test become positive. Lyme Disease IS a clinical diagnosis and the test are used to back up that diagnosis.

If you have many symptoms of LD, then common sense says to treat and see what happens. What would you have to loose? Many times treating leads to a positive test result.

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Fuel1212
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Timaca - definitely never any hard feelings. After all, you are taking your valuable time to help me and others.

My Mycoplasma was in the 300's and showed intermediate activity.

My HHV6 is pretty high at 3.67 anything greater than 1.10 is considered active

I am wondering what other testing I should get to check for other infections?

Also, I was told that only Lyme could make a cd57 score low? Mine is definitely low...this stuff is all so very confusing.

Hope everyone has a good weekend
Fuel

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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t9im
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Fuel:

I read on the LYMEMD blog (posts by a LLMD) that Alan Steere wrote a paper back in 1988 indicating band 41 by itself was significant as one could rule out the other spirochete diseases (sympilsi, wells, etc.) by symptoms.

So having 41 is important.

Equilavent or Indeterminate also used to be considered significant.

Of course then the CDC and IDSA thought the disease was being over diagnosed and over treated so they set up the barriers / guidelines to diagnosis and treatment.

If they bacteria wasn't there your body wouldn't be fighting it.

--------------------
Tim

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timaca
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Hi Fuel~ I would consider that mycoplasma may be an issue for you with an antibody titer in the 300s. Perhaps you may want to place a call and talk with Dr. Baseman's assistant: http://www.uthscsa.edu/micro/faculty/jbb/jbb.asp Maybe being on antibiotics is helping to kill off the mycoplasma and that is why you are feeling better.

An HHV-6 result in the 3s is not likely an issue. I've had Parvo and CMV results in the 7s and the doctors are not concerned. If you want to be sure consider getting retested at Focus Diagnostics lab (which is owned by Quest). See: http://chronicfatigue.stanford.edu/infections/herpes-tests.html for info on testing. Also: http://www.hhv-6foundation.org/research/hhv-6-testing

For other testing, do the tests suggested in the Stanford website. Click on each pathogen, and then click on "lab tests" and it will suggest which tests to run and at which lab.

I would also add to the EBV tests the IGA test at Focus: http://www.focusdx.com/focus/1-reference_laboratory/search_frame.asp?searchOptionScope=2&S1=1&S2=1&test=&sp=40480&Keyword=EBV%20IgA#an_40480

I was at the ILADS meeting in NJ in October and the CD 57 was discussed. Apparently there are other pathogens that can cause it to be low (can't remember what they are though...sorry). OK found something online about it...viral infections can also cause a low CD 57: http://www.truthaboutlymedisease.com/phpBB3/viewtopic.php?f=6&t=10

It is a puzzle to figure out. I remember thinking what the heck is Lyme? Do I have it? Then a doctor at a major medical institution that knows lyme well (he is a lyme researcher) diagnosed me with "probable Lyme". IV rocephin helped tremendously. So I knew I had a bacterial infection of some kind. Then I had tissue testing come back positive for HHV-6 and enteroviruses...and my thought was "What the heck are those? I've never heard of them!" And on and on it goes....but what I've learned is test for lots of things, treat what looks most obviously wrong.

Looking at your test results I would consider mycoplasma as a possible problem. I would put my eggs in that basket to start with. Get tested for other things too as you may have more than one pathogen causing an issue.

Best, Timaca

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timaca
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FYI, my HHV-6 testing was done at Focus and my antibody titers were 1:640. I was positive in some tissue testing that I had done (which turned negative after treatment).
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Fuel1212
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Good stuff, very interesting.

Now if I just had a money tree and a few willing veins I would be set [Smile]

Never thought my savings account would be for supplements and lab tests

--------------------
IgM- 31,34,39,83-93 IND
IgM- 41+

IgG- 31,34,39,83-93 IND
IgG- 41++

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Pinelady
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China says 41 is very significant. If you have 41 you have at least one bug with flagellin. Period. And the other options are just as bad if not worse than Lyme so what is the deal?

Get treatment and if that don't work in an immune system that has all its toll receptors turned off get more/other treatment...

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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Pinelady
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http://goanimate.com/movie/0XCA4kvs8vk4/1?utm_source=facebook&uid=0

I think this best describes the ignorance we have lived with for at least 30 yrs. Its is way past time to change...LOL

Pretty soon they are going to ask you if you would like one lump or two with your Alzheimers...LOL

Perhaps you had rather keep your MS or your Arthritis? For that well just kill 4 pathogens so you can keep us in business....LOL

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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Pinelady
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Pandora's box is open and the lies are pouring out.

All those stuck in the syndromes or psych hospitals not ever tested with tests that don't work and certainly never treated to cure are free with this research of finding multiple infectious organisms genes in those patients are free to seek the real treatment they need and can afford....

As our congress moves to deny and cut SS/Medicare in youth and other be prepared to fight for what the people really need now instead of all the junk they have profitted from at our expense for yr....

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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Pinelady
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http://www.polygenicpathways.co.uk/BLASTS.htm

Notice all infectious genes now found with psych illness.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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SilentStorm
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Hi, I found this very helpful forum while researching the ALS/Lyme connection/confusion. My boyfriend was recently diagnosed with ALS, needless to say we are devastated but determined to do as much research as possible before accepting this diagnosis. ALS is such a rare and horrific fatal disease that only affects 1-2 people out of 100,000 per year in the US, of course I wonder how/why. I am writing in hopes that someone can offer help/interpertation of his blood work. His initial blood work (done to rule out other diseases besides ALS) indicated "lyme Igg" positive. The doc made no mention of that. His WB test indicates 41 kd igg and 41 kd igm "*reactive". Having read so much for the past 2 days, I am confused....is 41 kd not a big deal? Should it be ignored, considering the lyme igg showed positive?

He has his first follow-up visit on Friday to discuss the trails meds for ALS, but until we rule out lyme and other diseases that mimic ALS, he will not take the ALS meds. Research indicates that the only FDA approved med for ALS has little or no affect on ALS and only extends patients lives by a few months.

Our next move is to find a LLMD and possibly retake the test again using igenex. His lab work was done by quest labs in NJ.

Thank you for all the valuable info I have received in just one day from this forum.

--------------------
If you desire something, offer something. Offerings can come in many forms: a gift, a prayer, a smile, flowers, food. Offer something from your heart.

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timaca
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Here is a quote from this link: http://www.canlyme.com/wb.html

"The band at 41 kDa corresponds to Bb's flagella (the whip like organelles used for locomotion -- Bb has several) and is one of the earliest to show up on the Western blots of Lyme disease patients. But for some reason it is also the most commonly appearing band in control subjects. This may be due to the fact that many people are exposed to spirochetes at some time in their lives and so their sera might cross react with this protein."

I don't recall the exact percentage but I'm pretty sure it is over 50% of people tested (healthy included) will test positive to band 41 on a WB. It is the antibody to the flagella and is non specific to lyme disease. See: http://www.canlyme.com/labtests.html

Best, Timaca

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SilentStorm
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Thank you Timaca. Some research will say 41 kd is important while others say not so. Is the lyme IGG test the same as the WB test? Again, the neurologist report says "lyme igg positive" (no other details, like #'s, bands, etc) Do you know what specifically but WB test shows 41 reactive. I'm confused.

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If you desire something, offer something. Offerings can come in many forms: a gift, a prayer, a smile, flowers, food. Offer something from your heart.

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timaca
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It would be helpful for you to get a copy of the lab report. I would think that "Lyme IgG positive" would mean that the IgG western blot was positive for lyme. This means that he tested positive for at least 5 of the 10 bands that the CDC requires for a positive lab test. This will help explain: http://www.canlyme.com/wb.html

If he has only band 41 I would not think that Lyme is the cause of his problems and I would look elsewhere for answers or get tested at SUNY Stonybrook to see what results turn up there.

Best, Timaca

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Lymetoo
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quote:
Originally posted by Pinelady:
China says 41 is very significant. If you have 41 you have at least one bug with flagellin. Period. And the other options are just as bad if not worse than Lyme so what is the deal?

Get treatment and if that don't work in an immune system that has all its toll receptors turned off get more/other treatment...

-
Exactly.

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--Lymetutu--
Opinions, not medical advice!

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Pinelady
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The results showed that the PCRs targeting the
OspA gene,
fla gene,
rrs gene, and
P66 gene detected
37 (6.8%),
74 (13.6%),
16 (2.9%), and
14 (2.6%) tick samples,
respectively. The PCR targeting the fla gene was the most sensitive method for the detection of B. burgdorferi s. l.
http://www.ncbi.nlm.nih.gov/pubmed/22494560

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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jmb
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I would not stop looking for answers but I sure would not discount Lyme as at least one of them.

--------------------
enjoy the day.

-jmb

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Pinelady
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Borrelia is all of them and all of them are Borrelia
New detection methods have found it in at least 80% of the pop.s Because it hides in the DNA as shown above.

http://www.youtube.com/watch?feature=player_embedded&v=yOno_2m_8LY

We the people should not be FORCED to wait until we are crippled with Lyme to treat the masses for the truth with the proper treatment=====Antibiotics and Stem Cells.

But the criminals who got us in this predicament of Autism now being diagnosed at 1 in 29 now trial Chemo/Rituxamab in all the syndromes to once again fill their pockets---not make you well. Because MS trials have already shown the disaster it to be.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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kim812
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I have been on this rollercoaster for almost 8 years now and am still not sure what my problem is.
I too only had a 41 band and many IND's after 4 WB Igenex tests. I even went to a well known LLMD in New York who confirmed to me that a 41 band does not indicate Lyme.

I was treated because I had symptoms but in my opinion they were not the ones that were commonly associated with Lyme. I had no brain fog, joint pain, headaches, etc.....

I had 6 months of Rocephin in which I got 85 percent better but soon backslide when I had to quit.
To make a long story short...I have had every test known for virus's and bacteria's and nothing is conclusive.
I had a high HHV6 titer at 21.44 but even then the doctor said that did not prove active infection.

I have been treating on and off for 6 years and still am not well...I suffer from chronic fatigue, neuropathy in my calf and feet and chronic sleep disturbance/poor sleep quality.

Maybe these are permanent from the original infection...whatever that might have been. I am currently on treatment for Bartonella even though in my heart I don't think there is any medication that is going to help me at this point...

Timaca...you have great information and it has always made me keep looking into other possibilities. I live in NH which is very endemic to Lyme but will never really know what happened to me in 2004 since I didn't ever see a rash or ever had any other positives on any Lyme testing.
I want to add that last year I did have a positive Elisa test but the doctor sent it out for confirmation and it then came back negative.

He also said the CD-57 is just an indicator of immune function and in no way represents active Lyme....
So I am still wondering what is wrong with me and if I will ever feel well again....

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Pinelady
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You can see in the PCR that the flagella is all that shows in most. We don't need anymore tests---its worthless.

If you are sick you have infections and it does not matter what all they are they still need killed and the gates of the immune system that lets them stay need closed to keep them out.

If it does not occur on its own---we need something that will. That is stem cells
and your own are working very well in those who can get it done.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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Pinelady
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http://www.ncbi.nlm.nih.gov/pubmed/21112481 2010 Oct Abstract

OBJECTIVE: Western blotting (WB; immunoblotting) is a widely used tool for the serodiagnosis of Lyme borreliosis (LB), but so far, no generally accepted criteria for its performance and interpretation have been established in China. The present study was designed to determine the criteria for standardized Western blot for the predominant species of Borrelia burgdorferi sensu lato in China, in which WB was produced with strain PD(91) as the representative strain attributed to predominant genospecies Borrelia garinii of Borrelia burgdorferi sensu lato.

METHODS: Approximately 13 bands between 14 and 100 kD were differentiated for strain PD(91) by using Gel-Pro analysis software. In a study with 631 serum samples (taken from 127 patients with Lyme borreliosis and 504 controls), all observed bands were documented. To establish criteria for a positive WB result for strain PD(91), receiver operating characteristic (ROC) curves were used.

RESULTS: The following interpretation criteria were recommended:

for IgG, at least one band of P83/100, P58, P39, P30, OspC, P17, P66, and OspA;

for IgM, at least one band of P83/100, P58, OspA, P30, OspC, P17 or P41.

In addition, syphilis, leptospirosis and other related diseases should be excluded when the positive band is P41 in IgM.

For IgG criteria, the sensitivity is 73.2%, the specificity is 99.4% and Youden index is 0.726; for IgM criteria, the sensitivity is 50.6%, the specificity is 93.1% and Youden index is 0.437.

CONCLUSION: Standardization of WB assays is necessary for comparison of results from different laboratories. Moreover, the criteria of other genospecies of Borrelia burgdorferi sensu lato should be determined in the future to complete the criteria of WB for the diagnosis of the Lyme disease in China.

Notice sensitivity is still what I consider low while the specificity is extremely high!!!!! Which means we may still need to kill some first to be seen on the testing.....

http://www.ncbi.nlm.nih.gov/pubmed/21106783 2010 Nov 24
Distribution of Borrelia burgdorferi sensu lato in China.
Abstract We
genotyped 102 Borrelia burgdorferi sensu lato strains isolated from
ticks, animals, and patients in 11 provinces in China by PCR restriction
... fragment length polymorphism (PCR-RFLP) amplification of 5S (rrf)-23S
(rrl) ribosomal DNA spacer amplicons and multilocus sequence analysis
************************************
http://www.canlyme.com/wb.html
The CDC criteria for a positive WB are as follows:

* For IgM, 2 of the following three bands: OspC (22-25), 39 and 41.

* For IgG, 5 of the following ten bands: 18, OspC (22-25), 28, 30, 39, 41, 45, 58, 66 and 93.
---------------------------------
All we are doing is letting them kill us by denying treatment for everything.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

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