Lymedin2010
Frequent Contributor (1K+ posts)
Member # 34322
posted
NEW & cheap DNA Lyme test ($150) for Lyme Disease.
This tests for multiple Borrelia strains and even Borrelia mayonii that was just recently discovered in 2016. Not approved yet, but the DNA testing is standard & approved in all branches of academia already (using Borrelial 16S ribosomal RNA gene sequence). This same type of DNA/RNA testing was also recommended & approved over standard antibody Zika virus testing and the info about it can be seen here.
At time 13:34 is the news coverage for Dr. Sin Hang Lee, the developer & crusader of the test, who is now suing the CDC for $57 million over neglecting to look into a viable testing method.
********************************************** They use PCR for Zika already! "Trioplex Real-time RT-PCR Assay
The Trioplex rRT-PCR is a laboratory test designed to detect Zika virus, dengue virus, and chikungunya virus RNA. The Food and Drug Administration (FDA) has authorized the use of this test under an Emergency Use Authorization (EUA). More information on the Trioplex rRT-PCR assay can be found on the Lab Guidance webpage."
********************************************** The PCR DNA/RNA testing is universally used in all science & industries. It is used throughout the world. The authorities use the same DNA testing for the Zika virus over the antibody testing for Zika too. It is the same thing!
It is an industry standard! Whether authorities want to quickly adopt the testing to quickly diagnose ill patients that are costly & who would otherwise be suppressed is where the issues is, not in the PCR testing though.
"Polymerase chain reaction (PCR) is a widely used technique used in molecular biology to exponentially amplify a single copy or a few copies of a specific segment of DNA to generate thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics .[1][2]
It was developed in 1983 by Kary Mullis,[3][4] who was an employee of the Cetus Corporation, and was later awarded the Nobel Prize in Chemistry along with Michael Smith in 1993 [5] for his work in developing the method. "
The study involved 94 blood samples of patients with suspected LD. From each sample, 1.5 ml of blood was used for the isolation of bacterial DNA and PCR real time am- plification and its equivalent, in nested version. The remaining part earmarked for serologi- cal testing. Optimization of the reaction conditions made experimentally, using gradient of the temperature and gradient of the magnesium ions concentration for reaction real time in nested-PCR and PCR version. RESULTS:
The results show that the nested-PCR real time, has a much higher sensitivity 45 (47.8%) of positive results for the detection of B. burgdorferi compared to the single- variety, without a preceding pre-amplification 2 (2.1%). Serological methods allowed the detection of infection in 41 (43.6%) samples."
********************************************** When I asked the testers if blood can be tested from overseas, this is what they said. "The answer is Yes. However, sending whole blood samples oversea is not practical. You may ask your local laboratory to centrifuge 3 mL of EDTA blood sample at 400 x g for 15 min to spin down the red and white cells.
Transfer 1 mL of the platelet-rich plasma to a 1.5 mL plastic tube to be further centrifuged at 16,000 x g for 10 min. to collect the platelets and the spirochetes, if any, in the pellet. Discard the supernatant completely. Send the plastic tube with the pellet to:
Milford Molecular Diagnostics
2044 Bridgeport Avenue
Milford, CT 06460
The sample can be send via Federal Express at ambient temperature. There is no sample collection kit. The request form can be found in our website under “Request a Test”.
Thank you for the interest in our laboratory. Looking forward to assist you with this important test.
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The
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