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» LymeNet Flash » Questions and Discussion » Medical Questions » Need help with Western Blot interpretation

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Author Topic: Need help with Western Blot interpretation
Farmerboy
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Member # 5401

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I have been on antibotics for 3 years. I've just received my 1st tests results from IGenex. Your interpretation would be helpful.

Thank You


Lyme IgM Western Blot Result

kDa
18 -
22 -
**23-25 -
28 -
30 +
**31 -
**34 -
**39 IND
**41 IND
45 -
58 +
66 -
73 -
**83-93 -


Lyme IgG Western Blot Result

kDa
18 -
22 -
**23-25 -
28 -
30 +
**31 -
**34 -
**39 IND
**41 +
45 -
58 -
66 -
73 -
**83-93 -

--------------------
Farmerboy

Posts: 22 | From Arkansas | Registered: Mar 2004  |  IP: Logged | Report this post to a Moderator
Michelle M
Frequent Contributor (1K+ posts)
Member # 7200

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Hi Farmerboy.

Western blots can be skewed by being on antibiotics when you test. You should be clear of antibiotics for a few weeks before testing.

Assuming you have symptoms compatible with lyme (and I reckon you do, since no one would take antibiotics for three years just for fun!), I think an LLMD would consider these bands meaningful...

IGM
30+
**39 IND
**41 IND

IGG
**39 IND
**41 +

While it's true the 41 band can be present in many people and isn't specific for lyme, it can also be from lyme, so I wouldn't discount it.

The 39 band is highly specific for borrelia burgdorferi.

The "IND" means they saw something -- not enough to say positive, but definitely NOT negative. Sometimes the very sickest people produce this response on western blot. Definitely do NOT take this to mean you're not infected.

Post us more of your story, please, and best of healing to you.

Michelle

Posts: 3193 | From Northern California | Registered: Apr 2005  |  IP: Logged | Report this post to a Moderator
Carol in PA
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Up for more comments.
Posts: 6956 | From Lancaster, PA | Registered: Feb 2004  |  IP: Logged | Report this post to a Moderator
Lymetoo
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Member # 743

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Well, looks like you still have it. Read this....maybe several times.

Western Blot explanation:
http://tinyurl.com/ffn3x

When you read it, be aware that "equivocal" is the same thing as "IND"...indeterminate.

--------------------
--Lymetutu--
Opinions, not medical advice!

Posts: 96239 | From Texas | Registered: Feb 2001  |  IP: Logged | Report this post to a Moderator
imanurse
LymeNet Contributor
Member # 7022

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OH Farmerboy.....

It's not nice to leave us hanging..

How are you? Did you see these comments to your post?

--------------------
**Eat Chocolate**

Posts: 942 | From USA | Registered: Mar 2005  |  IP: Logged | Report this post to a Moderator
   

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