Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
Lactoferrin, KCL, TNF alpha, angiotensin II, activation of PKC (Bb has a PKC INhibitor), growth hormone,arachidonic acid (AA), all stimulate tyrosine phosphorylation.
Why?
"Increases in tyrosine phosphorylation are essential to inhibit cell death."
"Phosphorylation involves the addition of a phosphate group to a protein, a small molecule, or other organic substance.
Because the phosphate acts to regulate so many enzymes and receptors, turning them off and on, phosphorylation is incredibly important.
Protein kinases catalyze phosphorylation, and phosphatases reverse the process.
Adding a phosphoryl can change a nonpolar hydrophobic protein into a polar, very hydrophilic molecule - in essence, changing its entire nature. Each phosphorylation reaction and its reverse requires ATP to power it.
While phosphorylation in general is fairly common, tyrosine phosphorylation is very rare.
However, tyrosine phosphorylated proteins are very easy to purify and therefore the few tyrosine phosphorylation sites on proteins are well-understood.
We do know that tyrosine kinase pathways are critical to proper cell growth, metabolic regulation, and normal differentiation, thanks to observations on tyrosine phosphorylation.
Currently, tyrosine phosphorylation is being investigated for its effects on the development of the fetus in everything from birds to people as well as its effects on things like sperm motility, cell death, and the formation of certain cancers.
Methionine -> homocysteine which triggers HMG CoA reductase. Alcohol raises homocysteine levels too. Homocysteine isn't all bad...if it is broken down further via nutrients to make the enzymes to do this. IF.
On the "flip" side,
If we are interested in INactivating NFkB in Langerhans cells, thus putting the brakes on TNF alpha and IL 1 Beta...
And for other diseases too...including ALS and HIV.
Would I take that drug? NOT A CHANCE.
Hand me the lactoferrin, curcumin (tumeric),ASA, Mg - with B vits, vitamin E, retinoic acid, Black raspberries, NAC, lipoic acid...
Curious that lactoferrin looks to contribute to tyrosine phosphorylation AND Inactivate NFkB.
OR we could simply "heat up" those langerhans cells prior to them reaching the lymph nodes.
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Areneli
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Marnie, Why do you think Mg drops in patients with LD?
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
Mg is an anti-inflammatory and anti-histamine.
Our initial reaction to Bb is an "allergic" reaction and we get stuck in this pathway. The response is primarily a lot of TNF alpha and interleukin 1 beta (IL1 B). For many reasons, TNF alpha is "protective". Like everything in nature, some is absolutely needed, but too much is not healthy. These are proteins...acidic.
Acids, negative charges , DO destroy pathogens, but...it takes the RIGHT negative charge to do this. We need to TARGET the pathogen. Bb does NOT like Mg...it wants Mn. It uses zinc which spells disaster because our OWN immune system needs this mineral. Bb does NOT like K, potassium either. It needs choline and uses the amino acid, tyrosine, etc.
In a pinch, in the body, Mn will sub for Mg. And...so will Al. Aluminum. Not good.
Mg INactivates HMG CoA reductase (liver enzyme) effectively stopping VLDL release from the liver. This is very low density liproproteins. They contain choline. We know Bb wants choline.
Unfortunately we need it too...it is part of bile salts and helps remove "heavy metals". It is part of the neurotransmitter, acetylcholine...without that...can't think "fast". It is one of the MAJOR neurotransmitters.
The reason why Mg breaks from ATP (mg-ATP)in the muscle cells at the OUTSET of this disease is for PROTECTIVE reasons. The body is trying to keep more Mg in "general circulation". Mg is not in abundance normally in general circulation. It is INTRAcellular...inside the cells, attached to ATP.
Once it drops, it continues to spiral down. It is very hard to get it back INTO the cells if the cellular pumps don't work.
The liver can MAKE cholesterol for us OR we can get the nutrients to do so from our foods...the "good" fats...the monoUNsaturated. The saturated fats (like Crisco in the cans) ALREADY have hydrogen in them. This prolongs the shelf life of the oil, but isn't good for us. Olive oil, canola oil, soybean oil are good.
We must have cholesterol as it forms the myelin sheath around our nerves, is needed to make OUR cell walls, breaks down to provide the sex hormones, etc. Unfortunately, Bb's cell wall is very similar...lipoproteins. It is actually amazing that the body "recognizes" this pathogen.
Basic microbiology...to destroy a gram negative pathogen, we must damage the cell wall (put holes in it) or better yet, prevent the formation in the first place to weaken the pathogen and then other things kick in to "finish the job" (osmotic pressure changes, even frequencies - ultrasound, etc.).
It takes Mg (along with Ca) to make antibodies. Ideally, healthy ones. We KNOW (pubmed abstract) that many of the antibodies we make to fight lyme - it takes a few days to make them - are damaged at the top. The area is called the "fab" portion. Antibodies look sorta like stalks of broccoli. When Mg levels are restored, the antibodies are once again a perfect "fit"...and they must be a perfect fit to work.
When Bb enters the body, it changes it's outer cell wall proteins which likely confuses our immune system since the above must be just right...a perfect fit. AND...in order for the antibodies to lock on, they need Bb to be in the cell wall form.
Mg plays a huge part in controlling our sugar level too. If you doubt, into your fav. search engine, type in the words, "magnesium diabetes".
Mg works with B6. (But all of the Bs work together.) They are water soluable. This means "excess" normally leaves the system in a couple of hours. We store just a little "backup".
Excess Mg leaves the system in about 2 hours also. This is the job of the kidneys...it is trying to maintain a really close blood pH level. Our body will use K (levels rise at the outset of lyme) and then our most abundant mineral, Ca - which will be pulled from our bones - to react with the acids to produce hydrogen. pH = the "potential of hydrogen".
Mineral + lots of acids = hydrogen. Sugar + lots of acids = hydrogen. We need both. In balance...which is, of course the tricky part.
Too much calcium isn't good.
I could go on, but you are probably in "overload". Suffice it to say...our body does NOT make mistakes. It is chosing to use Mg stores to fight. We know, in time, vitamin E stores (stored in fat) drop too. There is not a vitamin or amino acid or mineral that is not impacted which is why this gets so darned tricky.
This is one mean pathogen, but we know, from genetic research, exactly what it is doing.
It is fermenting sugar -> alcohol and following the glycolysis pathway. It is triggering the "cholesterol" pathway.
So does cholera...only faster.
We must attack both pathways simultaneously.
Only Mg does this.
IMO, while abx. do help (alter the immune response) and DO rid co-infections, they do not cure THIS infection. I respect your RIGHT to use abx. and
I will fight for your right to chose.
My goal is to find a CURE...a way to DESTROY every last spirochete, not simply to control the symptoms.
IF the "masses" are interested in Rxs...use the cholesterol lowering drugs. Those that INactivate HMG CoA reductase to prevent Bb from forming its cell wall (which is what we react to).
It takes TIME...many months...for this to work. First we need to destroy the pathogen and THEN the body can begin to repair the damage (to a large extent).
The key to this is keeping the vessels dilated and the blood "thin" in order to REACH the infection.
Did you follow the links above?
Thalidomide!!! YIKES.
If you are as old as me, you will remember the kids born without arms or legs in the 50s...
I don't think we have to chemically "poison" the body to heal.
Alternative treatments do work. Once again, it takes time and a real dedication to "cleaning up your act". This means eating healthy foods, drinking healthy beverages, getting some fresh air, some sun (a little), thinking positive, getting some exercise (walks or gentle exercises in a pool), that sort of thing. Laughter (and crying) are healing. So is prayer.
I know this isn't something any of us want to do. We all have our "vices" whether it be "addicted" to Pepsi (like me)...
But...If you want to get well...set a goal. Give yourself 2 years of "clean living" and set a plan of action.
Your health is in YOUR hands. God and your doctor and your family can help you in this time of need, but ultimately the choices are YOURS.
Keep an open mind and learn. You have "time".
You CAN get well. Never, ever forget that.
Posts: 9424 | From Sunshine State | Registered: Mar 2001
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Areneli
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I guess I am starting to subscribe to your ideas, Marnie.
So what is the best way of bringing this intracellular Mg to normal level?
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
Areneli,
There are MANY supplements I would take and I would Rife, use an ozone sauna (on occasion because it is very expensive), and I definitely would use a far infrared sauna. All of these alternative healing devices are fortunately available in my state. I have researched them in depth and understand HOW they can help.
As far as supplements go, IN MY OPINION, the following are absolutely critical and here is what I, PERSONALLY, would do:
Restore my gut. Healing STARTS in the gut.
I'd take 1 Zantac and 2 Pepto Bismol chewable tablets at bedtime for 1 week only. Meanwhile, before breakfast and before lunch, I would take loading doses of Essential Formulas probiotics with a full glass of water. That means 5 capsules at a time. I would be using the entire box in one week. They are expensive ($60),but they also contain not only the beneficial bacteria, but nutrients to "feed" them. Later, I'd use another brand of probiotics and less, but I'd still keep the "good guys" level up.
Week #2 and ongoing:
Mg citrate 100mg Lecithin capsule B 50 complex (each B is 50mg)
I'd take the above 3 every 2 hours for 6 doses per day. I'd put the doses in a "weekly" pill box, filling 6 of the 7 days. I'd take the 3 pills loaded into each "day" every even hour...like 8am,10am,12noon,2pm,4pm,6pm.
This is Valletta's patent, modified. because we can't purchase Mg pyrophosphate.
I would NOT CHEAT. TIMING IS CRITICAL. I would be trying to MAINTAIN a slightly high(ish) level of Mg in my blood stream for as many hours a day as possible. In addition, I will be raising my pH with these (minerals + acids = hydrogen).
NOW (a brand) makes Mg citrate in 200mg. I would cut them in half with a pill cutter. I would purchase lecithin from Carlsons (big brown glass bottle) and keep the bottle in my refigerator. I would get B50 complex from Solray.
I would expect to feel a LITTLE better (noticeable) within one week.
As far as other supplements go, here's a list of those I PERSONALLY believe are very very helpful (without going into the "whys"):
Vitamin E as "Gamma E" from Jarrow Formulas CoQ10 200mg Selenium 200mcg
Those 3 together with a meal.
Digestive enzymes GlucoReg by Solaray (chromium + acids) SOD by Solaray (contains all 3 antioxidant enzymes) AlkaMax by TriMedica (only IF my K level was okay...it comes with free pH testing paper) Chorella Juvenon ($ but worth it) 5HTP Omega 3s Pharmanex daily vitamin-mineral packets (esp. for the Ginkgo in them) Host Defense by New Chapter (mushrooms...beta glucans) Phosphatidyl Serine (300mg divided doses to restore the HPA axis) Zinc+vitamin C lozenge Metamucil Baby ASA d ribose Lactoferrin Leutin from Carlsons Creatine (in the news re: muscular dystrophy, helpful) Whey protein
While researching this morning...I have come across some VERY interesting additional information.
To be posted.
It appears the INTRACELLULAR level of K impacts the enzyme aldehyde dehydrogenase.
We MUST get K back IN the cells also.
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Areneli
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I am one the testers for Nutrasilver. With this silver treatment several of my symptoms got reduced (which is great news) but some didn't even budge. The resistant symptoms were: muscle pain of feet and hands (mainly dorsal parts) and eye pain (kind of ocular muscle pain- eyes are in pain when rolled). I assumed that I also had some coinfections with Bartonella or Babesia and the silver was unable to fight them. That is why I had these muscle pains.
But I think I was wrong...
A ND from Texas phoned me and suggested detoxication with magnesium. I was supposed to increase my dose of magnesium to such a level that I would end up with 2-3 bowel movements per day. I used nothing special, some Magnesium oxide I found in my kitchen cabinet - some leftovers from better times.
I took about 1600 mg of magnesium + 1 multivitamin per day and MAGICALLY within just 24-48 hours some of muscle pain did go away. I mean 70% improvement. Particularly the pain in dorsal parts of hand and feet is gone 100%. There is still some residual pain in left eye and some in fingers and toes but I have been taking magnesium just for a few days and I know I should give it more time.
Just to see things in perspective, I had these muscle pains for over a year so I can hardly think that it was a random improvement. It must be because of magnesium. Also I doubt that a detoxication was taking place because I have never reached the magnesium level so I could have more than one 1 bowel movement per day. The dose of magnesium I take is still too low for that.
At this point I realize that I was terribly deficient with magnesium and it was blocking my recovery ... phew!
I should also add that my doctor tested me for level of Mg in blood several times during the last year and it was always normal.
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posted
Areneli - "...I should also add that my doctor tested me for level of Mg in blood several times during the last year and it was always normal. "
In response to the above, my blood tests kept coming back ok. It wasn't until my LLMD had me do an intracellular test through SpectrCell Laboratories that I found functional deficiencies of Folate, Magnesium, Glutathione, Glutamine, and a Fructose Sensitivity. I was also very low in B2, B12, Serine, Choline, Inositol, Carnitine, Zinc and CoQ10. Test 9/06.
Very little muscle pain and burning since 1/07 due to (IMO)specific supplementation per LLMD(Mg and B vitamins, I also take Nutriferon, Reliv, Buhner protocol and other specific supplementation). LLMD also has me taking Doxy since 1/07, before then, it was only a natural protocol.
These symptoms do come back at times but no where near the levels as before.
Marnie, my case over the past two years and more specifically this last year, seem to give support to your conclusion about Mg.
Marnie, what about the muscle weakness, does that eventually get better as your body heals?
Thanks,
Stan
Posts: 59 | From Indiana | Registered: Jun 2006
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
Melatonin and alcohol (all spirochetes ferment sugar to alcohol) ALSO lower Mg...so it looks likt a significant amt. is needed, but not once a day massive doses...just keep the level SLIGHTLY elevated. B6 is absolutely essential.
I am not surprised at all by the intracellular testing results!
Not at all. (Although I am somewhat surprised B1 wasn't low too.)
To build muscle (and strength) which contain LOTS of mitochondria (we need a lot of powerhouses to power our muscles)...in the news recently for muscular dystrophy...creatine.
There is an imbalance of the amino acids in lyme. Bb looks to be using tyrosine, while we are fighting with tryptophan.
Many other "this versus that" happening. Mn vs Mg is another. I suspect Zn vs vitamin C is another.
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Areneli
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How do you do intracellular testing?
Do you need to have a biopsy of some tissue?
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posted
My LLMD had to order the tests and he used SpectraCell Laboratories. It was a simple blood draw that was done at my hosptial and sent to the lab. I don't think it can be done without an MD's orders.
My insurance wouldn't cover it so it cost me around $400 but now I know specifically what to supplement. They said it was "medically not necessary" even though my LLMD ordered it. I am still appealing the decision. The total cost was over $1000 if insurance would have paid.
Posts: 59 | From Indiana | Registered: Jun 2006
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Areneli
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I am puzzled how it was done from blood alone- I mean the test itself.
Perhaps they tested the insides of erythrocytes.
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A: The company was founded in 1993 to commercialize Functional Intracellular Analysis (FIA). With the support of clinicians throughout the country, it has become the premier provider of functional testing.
Q: What is FIA�?
A: Functional Intracellular Analysis (FIA) is a next generation blood test for measuring specific vitamins, minerals, antioxidants, and other essential micronutrients within an individual's white blood cells (lymphocytes). It is the gold standard for this type of test.
Q: Who developed FIA�?
A: William Shive, Ph.D., chairman of the department of biochemistry and a researcher in the field of nutrition at the University of Texas, began work on a diagnostic test for clinicians in 1978. His work was strongly influenced by researchers like Roger Williams, Ph.D., the progressive and eminent scientist who wrote Biochemical Individuality and discovered vitamin B5. SpectraCell later licensed this technology from the Clayton Foundation for Research.
Q: How was FIA developed?
A: Dr. Shive first identified appropriate cells for the functional assays. He selected lymphocyte cells because they are simple to collect (via venipuncture), easily isolated from other whole blood components, and maintainable in culture for days to weeks.
Q: How do lymphocytes provide a nutritional history?
A: Most lymphocytes obtained by venipuncture are in a resting state in terms of cell division. Since they have a 4- to 6-month lifespan, the nutrient levels accumulated in these lymphocytes represent a history of an individual's nutrient status. This situation is analogous to using HbA1c measurements to approximate a diabetic person's glucose levels over the months preceding a test. Thus, lymphocytes provide a history rather than a snapshot of nutrient intake.
Resting lymphocytes can be stimulated by a lymphocyte-specific mitogen to undergo cell division and grow in culture. The degree of growth that the lymphocytes can maintain is directly related to the nutrients they have available. Thus, the FIA provides a functional intracellular analysis of nutrient status accumulated in human lymphocytes over their resting lifespan.
Posts: 59 | From Indiana | Registered: Jun 2006
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clairenotes
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posted
More priceless information.
Claire
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