Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
If this has been already posted...forgive me...I'm flying fast...
Only rabbit serum and tick saliva have been reported to be chemoattractants for B. burgdorferi.
These complex biological mixtures are limited in their utility for studying chemotaxis on a molecular level.
Here we present a modified capillary tube chemotaxis assay for B. burgdorferi that enumerates cells by flow cytometry.
Initial studies identified N-acetylglucosamine as a chemoattractant.
The assay was then optimized with respect to cell concentration, incubation time, motility buffer composition, and growth phase.
Besides N-acetylglucosamine, glucosamine, glucosamine dimers (chitosan), glutamate, and glucose also elicited significant chemoattractant responses, although the response obtained with glucose was weak and variable.
Serine and glycine were nonchemotactic
Applied and Environmental Microbiology, February 2007, p. 1180-1188, Vol. 73, No. 4
The ticks are attracted to CO2 as we exhale (info for newbies).
[ 22. March 2007, 10:18 AM: Message edited by: Marnie ]
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Marnie
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posted
Up...curious about what Bb is NOT attracted to.
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treepatrol
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From what i have read ticks attracted to C02 slightly attracted to body heat yes,and attracted to sick hedgehogs because of what is in there excrement and the odor they give off.
Anyway borrelia like me any clue sometimes mostly seems they like sugar and do they have this like HIV aids virus and other virus's bateria ?
These host-derived proteins offer some protection to HIV; however, the acquisition of host DAF can only partially explain the measured resistance of HIV to complement-mediated lysis. It turns out that the human complement regulator factor H is even more crucial for HIV's resistance. Factor H is not a membrane-bound protein, so HIV does not acquire it during cell budding. Rather, one of its two major surface glycoproteins, gp41 (the other is gp120), binds to serum factor H (Pinter et al, 1995). The crucial importance of this interaction in mediating HIV resistance to complement is underscored by the finding that antibodies specific to the factor H binding region on gp41 allowed for efficient complement-mediated lysis. (Stoiber et al, 1996) This finding and others which demonstrate interactions between HIV surface proteins and complement regulatory molecules (Stoiber et al, 1995) are potentially important because they suggest a particular HIV vaccine strategy. [See my next section, next section
Lest the situation seem overly simple, HIV interacts with the complement system in a variety of other ways as well. For example, like many other pathogens, HIV binds to certain complement receptors and uses them to gain entry to cells. The full interaction of HIV with the human complement system remains to be elucidated.
Sialic acid Seems like acids play big role in binding in these vulnerable places displacing the normal binding of other virus,bacteria etc in this H factor area???
Borrelia burgdorferi, a spirochete transmitted to human hosts during feeding of infected Ixodes ticks, is the causative agent of Lyme disease. Serum-resistant B. burgdorferi strains cause a chronic, multisystemic form of the disease and bind complement factor H (FH) and FH-like protein 1 (FHL-1) on the spirochete surface. Here we report the atomic structure for the key FHL-1- and FH-binding protein BbCRASP-1 and reveal a homodimer that presents a novel target for drug design.BbCRASP-1 homodimer cure?
All has to do with factor H binding site gets messed up MAC process.
This is hard to follow
-------------------- Do unto others as you would have them do unto you. Remember Iam not a Doctor Just someone struggling like you with Tick Borne Diseases.
-------------------- Do unto others as you would have them do unto you. Remember Iam not a Doctor Just someone struggling like you with Tick Borne Diseases.
CaliforniaLyme
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Member # 7136
posted
ME*)!!!
-------------------- There is no wealth but life. -John Ruskin
All truth goes through 3 stages: first it is ridiculed: then it is violently opposed: finally it is accepted as self evident. - Schopenhauer Posts: 5639 | From Aptos CA USA | Registered: Apr 2005
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Marnie
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Member # 773
posted
I thought about joking and slipping in...
"attracted to tin cups".
Okay...remember when I said good old ASA (do you know what ASA is?) and getting NO levels up as being really vital?
Thin the blood and dilate the vessels.
Very important.
Curious about serine...as in the HPA axis connection....This bugger wants cortisol to soar.
Side note: to trap ticks (for counting purposes i.e. surveillance, they use CO2 traps.
DE sprinkled around your house (outside) should help control tick populations, but do NOT BREATHE it!!!
Off track again...
Is HIV PFK dependent?
Type in (HIV glucose) into a search engine.
MANY pathogens love sugar...just like us ;-)
So do cancer cells.
Looks like we gotta get PFK1 levels down, but that is tricky because our brain needs a source of glycogen...
Brain cells MUST have glycogen.
When our sources of glycogen are low...what happens?
We start breaking down fat to supply the amino acids to MAKE glycogen.
Finally...we will breakdown protein...but only as a last resort.
As I understand it...so far ;-)
Re: Factor H
C-reactive protein inhibits pneumococcal activation of the alternative pathway by increasing the interaction between factor H and C3b.
See why this protein sometimes goes up?
Like I've said before...the body is working hard to find every route possible to fight.
Other things can activate factor H too...as well as break the bond between Osp E and factor H.
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randibear
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posted
Somebody said they feed on sugar, is that right??
-------------------- do not look back when the only course is forward Posts: 12262 | From texas | Registered: Mar 2007
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
Randi...all spirochetes ferment sugar to ethanol.
Bb uses an enzyme called PFK. It is phosphofructokinase)...see "fructo" as in fructose? Phospho as in phosphate?
Bb is "PFK dependent". This enzyme controls the glycolysis pathway. This is one pathway to make ATP. Not a good one. We don't make ENOUGH ATP that way.
It is better if we use oxygen to make ATP, but Bb is not fond of oxygen.
So...yea...in a manner of speaking, you are slightly drunk...all the time and your body has to breakdown ethanol via 2 enzymes...ongoing.
Women have less of the first enzyme, which is why most men can drink us gals under the table.
[ 23. March 2007, 09:56 PM: Message edited by: Marnie ]
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quote:Originally posted by Marnie: Okay...remember when I said good old ASA (do you know what ASA is?) and getting NO levels up as being really vital?
Thin the blood and dilate the vessels.
Very important.
Curious about serine...as in the HPA axis connection....This bugger wants cortisol to soar.
What was ASA?
You mention NO, vasodilation -- also another post I thought I saw you mention phosphodiesterase inhibitors -- geez, it sounds like you are talking about Viagra (PDE5 inhibitor)?
300mg of phosphatidyl serine on an empty stomach always makes me feel good... probably a lot better than it should....
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
ASA...aspirin...Acetylsalicylic acid. Blood thinner. Not much is needed. Not much at all.
NO...nitric oxide. Yes, dilates vessels. (So does exercise.)
Yes...Viagra inhibits PDE5. Recent research indicates inhibiting PDE4 might be valuable for many diseases.
(The various PDEs all have different functions.)
Inhibiting PDE4 looks to increase cAMP (between ATP conversion to ADP..losing a phosphate) and by keeping cAMP levels up, energy is released.
Normally Mg-ATP increases cAMP, but since Mg is low...researchers looked for another way.
Ultimately it impacts PFK1.
Bb is PFK dependent.
It appears a flavonoid in lemons and oranges works the same. It is called hesperidin. And yes, it is available OTC.
From another angle...lactoferrin (in colostrum and added to formulas) also seems beneficial to a huge extent. There are ALL the essential amino acids in lactoferrin and this supplement supposedly binds iron (greater than transferrin) and possibly zinc.
Your comment about phosphatidyl serine and the one shot dose was curious.
I have read...100mg 3x/day and any more or any less didn't work/make a difference.
Your experience (300mg "hit" at one time) seems to conflict what I have read.
Do lyme patients need more?
I wonder.
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quote:Originally posted by Marnie: ASA...aspirin...Acetylsalicylic acid. Blood thinner. Not much is needed. Not much at all.
NO...nitric oxide. Yes, dilates vessels. (So does exercise.)
Yes...Viagra inhibits PDE5. Recent research indicates inhibiting PDE4 might be valuable for many diseases.
Hadn't see acetylsalicylic acid abbreviated as ASA before, sorry.
Yeah PDE4 inhibitors are supposed to do cure depression, cure COPD, shrink brain tumors, work as anti-inflammatories, fix asthma and lung disease... probably give you a whiter, brighter smile too
I found the abstract pointing to hesperidin as a PDE inhibitor... "vasorelaxant effects of this flavenoid." It apparently also enhances sleep.
I am out of my depth on "pyrophosphate-dependent phosphofructokinases" and how it relates to Bb...
I am totally scratching my head about how all this connects -- I feel like I just dropped into the middle of a conversation -- but you have given me some interesting things to look up.
I tried to take a step back to see where you were coming from about borellia & the lining of blood vessels, but I went into a huge circle: searching on "borrelia endothelial" and finding an article stating that Bb crosses the BBB by using matrix metalloproteinases. Then I google "matrix metalloproteinases" and find out that low-dose doxy inhibits them! So is doxy bacteriostatic, bacteriocidal, or is it just keeping Bb from getting into your endothelium and brain?
As for the 300 mg PS, there are bound to be personal differences in anything... me, I like to FEEL the supplement working so 300 mg works for me... the empty stomach helps a great deal, though technically I suppose it depends on whether you're making the serine compete with any other amino acids in your stomach.
It will totally whack down your cortisol and hit you like 10 mg of Valium...
Thanks for the interesting comments.
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