The antimicrobial agent melittin exhibits powerful in vitro inhibitory effects on the Lyme disease spirochete.
Lubke LL, Garon CF. Rocky Mountain Laboratories Microscopy Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA.
Borrelia burgdorferi has demonstrated a capacity to resist the in vitro effects of powerful eukaryotic and prokaryotic metabolic inhibitors.
However, treatment of laboratory cultures on Barbour-Stoenner-Kelly medium with melittin, a 26-amino acid peptide contained in honeybee venom, showed immediate and profound inhibitory effects when they were monitored by dark- field microscopy, field emission scanning electron microscopy, and optical density measurements.
Furthermore, at melittin concentrations as low as 100 microg/mL, virtually all spirochete motility ceased within seconds of inhibitor addition.
Ultrastructural examination of these spirochetes by scanning electron microscopy revealed obvious alterations in the surface envelope of the spirochetes.
The extraordinary sensitivity of B. burgdorferi to mellitin may provide both a research reagent useful in the study of selective permeability in microorganisms and important clues to the development of effective new drugs against lyme disease.
PMID: 9233664
-------------------- There is no wealth but life. -John Ruskin
All truth goes through 3 stages: first it is ridiculed: then it is violently opposed: finally it is accepted as self evident. - Schopenhauer Posts: 5639 | From Aptos CA USA | Registered: Apr 2005
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
It didn't work for Tammy of Naples, Fl.
Likely may have to do with pathogen load and other factors.
Bb's motility is NaCl dependent. Documentation follows:
"It is also interesting that
motility of B. burgdorferi requires a high concentration of NaCl
(My note: BOTH transported by high levels of glutamate!)
and pH 7.6, which are the normal physiological conditions for interstitial fluids.
In any case, our data suggest that chemotaxis may indeed be important for the pathogenesis of B. burgdorferi."
posted
I have a local guy who does the bee venom thing... is this something I should consider as an extra therapy in addition to my abx?
Posts: 151 | From ohio | Registered: May 2007
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Marnie
Frequent Contributor (5K+ posts)
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posted
There is a NFkB (cell call to alarm) inhibitor "related" to bees...
Read the above carefully...very carefully (hint - see what INHIBITS motility!!!)
Posts: 9481 | From Sunshine State | Registered: Mar 2001
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GiGi
Frequent Contributor (5K+ posts)
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posted
Bee Venom was part of my treatment modalities back in the late ninetees.
This is how the Bee Venom was and still is applied (with Neural Therapy):
Neural Therapy with Bee-Venom. by Dr. Dietrich Klinghardt, M.D., PhD
Neural Therapy(NT) is a treatment of dysfunction(s) within the autonomic nervous system(ANS). NT was developed in Germany in the early part of this century by two physicians, Walter and Ferdinand Huneke. Historically it involves the injection of scars, glands, trigger points, acupuncture points, vascular structures, ligaments and autonomic ganglia with procaine.
Bee Venom Therapy(BVT) is a treatment modality which may be thousands of years old. It involves either the application of live bee stings to the skin of a patient or in more recent years the injection of bee venom into the skin with a hypodermic needle.
The author began using bee venom in his practice more than 12 years ago and found the combination of BVT and NT a powerful and effective treatment modality. This combination appears in properly selected cases clearly more effective than either BVT or NT alone.
Method: the safe parameters for this treatment are the same as for the single modalities: in the first treatment no more than 0.4ml of BV* are used in a syringe together with the desired amount of Procaine solution. No more than 25 ml of 1 % preservative free Procaine are used per treatment. The amount of BV can be gradually increased, if the patient is seen twice/week regularly up to 2.5 ml/treatment. The upper limit for the use of Procaine does not change. With the use of BV in NT it is usually quite effective, to use only small amounts of BV (0.1-0.2ml) with each treatment. In order to reduce the stinging sensation during the injection, 1 ml of 8.4% Sodium Bicarbonate can be drawn up per 10 ml of Procaine. This reduces the effect of the injection somewhat but enhances the compliance of the patient because it makes the treatment less painful and therefor easier tolerated.
The following NT procedures are clearly enhanced by adding BV to the solution:
Scar injections Segmental Therapy injections ( i.e. liver, kidneys, thyroid etc.) Skin injections over painful joints (bunion, jaw-joint, etc.) Skin injections over painful areas Skin injections for hair loss We found no benefit, when injecting BV into any structure below the skin, such as autonomic ganglia, joints, trigger points, intravenously etc.
Clinical observations: the following conditions appeared to respond most dramatically and often lastingly to the combination of NT with BV:
1. Acute or chronic joint pain (segmental therapy and all scars)
Post-herpetic neuralgia (Herpes zoster in the chronic stage, not in the acute stage) Post 3rd degree burn pain (segmental therapy) Fibromyalgia (injections over the areas of tenderness, all scars, adrenals, thyroid) Kidney failure (segmental therapy over the kidney area twice/week) Depression and chronic fatigue(segmental therapy to scars, skull, thyroid and adrenal area) Facial pain ("TMJ" or "TMD") Premature aging and hormonal imbalances (same treatment as 6.) Treatment schedule: any condition may respond to a single treatment or may require a series of injections. These should be given initially twice/week, later once/week. If there is no sign of improvement after 4 treatments, I discontinue this modality.
During this lecture the author will present case presentations and technical details of his treatment approach.
* 0.1 ml of bee-venom (BV 20) contain approximately the same amount of BV as a real bee-sting, delivered by a honey bee to the skin of a human
Neuraltherapy.com
The Treatment of Lyme Disease with Bee Venom by Dietrich K. Klinghardt, M.D., Ph.D. abbreviated - full text available through The Institute of Neurobiology at 425-637-9339 or [email protected]
Introduction Lyme disease has become, after AIDS, probably the fastest spreading infectious disease. ``Classical'' Lyme disease is a bacterial infection caused by a spirochete, Borrelia burgdorferi, which is passed to the patient by a tick bite. Since several other infections that cause similar symptoms can be transmitted by the same tick bite, and other infectious agents not transmitted by a tick can cause similar symptoms, the term ``New Lyme Disease'' is used by most holistic physicians. Lyme disease is not only a frequent underlying causal factor in chronic human illness, but also extremely common in pets, especially in dogs and horses.
The following microorganisms have to be considered when making the diagnosis of ``New Lyme Disease." 1. Borrelia burgdorferi; 2. Babesia microti (a protozoan intracellular invader); 3. Ehrlichiosis; 4. Mycoplasma pneumoniae (associated with MS, ALS, Chronic Fatigue and Fibromyalgia); 5. Chlamydia pneumoniae; 6. Bartonella henselae; 7. Rickettsia rickettsiae.
The following symptoms can be caused by Lyme disease: 1. Chronic Fatigue (more severe in the early afternoon); 2. Lack of endurance; 3. Non-healing infections in the jaw bone, devitalized teeth, dental pain; 4. Fibromyalgia; 5. Joint pains (especially in the spine); 6. Multiple Chemical Sensitivity; 7. Cranial Nerve Problems: o Facial nerve: Bell's palsy (60 % are caused by Lyme disease, 30 % by one of six common viruses from the herpes family, such as EBV, Herpes simplex type I, type II, type 6 etc); o Trigeminal nerve: sense of vibration in the face, TMJ and facial pain, headache, tension and cramps in the face/skull/jaw; o Ears (VII, VIII): tinnitus, vertigo, and hypersensitivity to noise; o Eyes (II, III, IV, VI): decreasing and changing eye sight (fluctuates during the day), light sensitivity, floaters; o Vagus (X), Glossopharyngeal nerve (IX) and Hypoglossus (XII): difficulty swallowing, faulty swallowing, reflux, hiatus hernia, heart palpitations, supraventricular arrythmias. 8. CNS problems: o Physical: epileptic seizures, insomnia, tremor, ataxia, movement disorders (torticollis, etc.); o Emotional: irritability (key symptom in children), depression, bi-phasic behaviour (manic-depression), bouts of anger, listlessness; o Mental: confusion, difficulty thinking, poor short term memory, increasingly messy household and desk, difficulty finding the right word, feeling of ``information overload;'' o Mixed pictures: can resemble or imitate any known psychiatric illness. 9. Peripheral nervous system problems: o Paraesthesia, burning, vibration, numbness, shooting pains. 10. Pelvis: interstitial cystitis, prostatitis, sexual dysfunction, loss of libido, pelvic pain, menstrual disorders. 11. Immune system failure: with all known secondary illnesses such as herpes virus infection, intestinal parasites, malaise. 12. General symptoms: hair loss, loss of zest for life, sensitivity to electric appliances.
Laboratory Testing
Until recently laboratory testing has been unsatisfactory with a detection rate of probably below 30 %. In the past it was believed the laboratory evaluation of the spinal fluid was a reliable way to confirm or refute the diagnosis of Lyme disease. This has been proven wrong. The test with the broadest detection rate, the Western Blot ELISA test, has low specificity. The test with the highest specificity but with a fairly low detection rate was the PCR test. The B. burgdorferi is a master at evading the body's immune system and evading laboratory detection by modulating and changing its surface antigens. It can form a cystic stage, which is resistant to antibiotics, evades laboratory detection, and gives birth to healthy spirochetes once the antibiotics are discontinued.
A new test has recently become available: the C6 Lyme Peptide ELISA test (BBI Clinical Laboratories, Tel.: 1-800-676-1881 or 1-508-580-1900, test code: 556 - C6LPE. The test is based on the discovery of six (6) peptides on the surface of the spirochete, which are consistently present and do not evade detection by the laboratory as many of the other surface antigens of B. burgdorferi do. This test detects all B. burgdorferi strains and genospecies. It is highly specific and more sensitive than conventional tests for chronic Lyme disease. It is also sensitive in early Lyme disease (which used to be problematic) and can be used for accurate antibody results for Lyme vaccinated patients. The treatment protocol has intentionally been removed. All treatment should be supervised by a medical professional. RESOURCES Acupuncture charts -- Apitronic Services, Tel.: 604-271-9414 Dried peptide extracts -- Chisolm Biological Laboratory, Tel.: 803-663-9618 / ext. 9777 Herbs -- Monastery of Herbs, Los Angeles, Tel.: 818-360 4871 Herbs protocol -- Dr. Zhang, MD, LAc of New York, web site: www.dr-zhang.com Lab test -- C6 Lyme Peptide ELISA test -- BBI Clinical Laboratories, test code: 556 - C6LPE, Tel.: 1-800-800-676-1881 or 508-580-1900. SoftSting -- Apitronic Services, Tel.: 604-271-9414 Neural Therapy and Autonomic Response Testing workshops and resources - The Institute of Neurobiology, PO Box 5023, Bellevue, WA, 98009, USA, Tel.: 425-637- 9339, Fax: 425-637-9669, E-mail: [email protected], www.neuraltherapy.com Procaine (preservative free) ApotheCure Pharmacy, Tel.: 800-969-6601 Fruitvale Pharmacy (Canada): 250-367-9331 McGuff Compounding Pharmacy Services: 877-444-1133 VeneX-10 and VeneX-20 -- Tel.: 604-271-9414 RESOURCES FOR INFORMATION Books, Booklets and Literature Beck, B. F., MD (1997) The Bible of Bee Venom Therapy. Health Resources Press, Inc., Silver Spring, MD, USA, book, ISBN 1-890708-03, pp. 238. Reprint of the original 1935 edition of Dr. Beck: Bee Venom Therapy - Bee Venom, Its Nature, and Its effect on Arthritic and Rheumatoid Conditions. (available from Apitronic Services: Tel.: 604-271- 9414) Broadman, J., MD (1997) Bee Venom - The Natural Curative for Arthritis and Rheumatism. Health Resources Press, Silver Spring, MD, USA, book, ISBN 1-890708- 01-3, references, index, glossary, foreword by Harold Goodman, DO, pp. 224 (available from Apitronic Services: Tel.: 604-271-9414) Klinghardt, D. K., MD (1990) Bee Venom Therapy for Chronic Pain. The Journal of Neurological & Orthopedic Medicine & Surgery, Vol. 11, No. 3, pp. 195-197 Klinghardt, Dietrich, MD (1999) Treatment Protocol for Bee Venom Therapy. Apitherapy Education Service - Apitronic Services, Richmond, BC, Canada, booklet, 11 pp. Lubke, L. L. and Garon, C. F. (1997) Bee Stings as Lyme Inhibitor. J. Clin. Infect. Diseases, July, 25 Suppl. 1, pp. 48-51 Marinelli, Rick, ND and Klinghardt, Dietrich, MD (1999) Methodology for Injectable Bee Venom Therapy. Apitherapy Education Service - Apitronic Services, Richmond, BC Canada, 12 pp. Mraz, Charles (1994) Health and the Honeybee. Queen City Publications, Burlington, VT, USA, ISBN 0-9642485-0-6, pp. vii+92 (available from Apitronic Services: Tel.: 604-271-9414) Organizations American Apitherapy Society, Inc., 5535 Balboa Blvd., Ste 225, Encino, CA 91316 USA, Tel.: 818-501-0446, Fax: 818-995-9334, e-mail: [email protected], web page: www.apitherapy.org Neural Therapy and Autonomic Response Testing workshops and resources - The Institute of Neurobiology, PO Box 5023, Bellevue, WA, 98009, USA, Tel.: 425-637- 9339, Fax: 425-637-9669, E-mail: [email protected], www.neuraltherapy.com Internet Resources The American Academy of Neural Therapy www.neuraltherapy.com Bee Venom Therapy Supplies and Books www.beevenom.com Apitherapy Reference Database www.saunalahti.fi/~apither/ Bee Venom Therapy Supplies and Books Bee venom products and therapy related books, literature and Apitherapy Education Service. Apitronic Services 9611 No. 4 Road Richmond, BC Canada, V7A 2Z1 Ph./Fax: 604-271-9414 e-mail: [email protected] www.beevenom.com Conversion Table 0.10 ml = 0.10 cc 0.60 ml = 0.60 cc 0.20 ml = 0.20 cc 0.70 ml = 0.70 cc 0.30 ml = 0.30 cc 0.80 ml = 0.80 cc 0.40 ml = 0.40 cc 0.90 ml = 0.90 cc 0.50 ml = 0.50 cc 1.00 ml = 1.00 cc
Posts: 9834 | From Washington State | Registered: Oct 2000
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Vanilla
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posted
I met someone at my first LLMD's office who was yet undiagnosed first she thought she had MS and then now thought it might be LD.
She said until she tried bee venom she could not get out of bed so whatever she had it worked for her.
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CaliforniaLyme
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Member # 7136
posted
I am always pro-alternative therapy if it works! We had an ALS guy who died of Lyme in the old group and his wife like a few wives of ALSers btw had MS and she was greatly helped by this. Was only person I knew who tried it but she was hardcore MS and it really helped her, so I have always been curious of it since then and supportive of those who investigate it (go guinea pigs go!*)! I let other people do my research for me*)!! It can't hurt so- well- it DOES hurt- but just as much as a bee sting*)!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! or two or five!! But this may be why it helps some people- a great abstract I thought- Best wishes all, Sarah
-------------------- There is no wealth but life. -John Ruskin
All truth goes through 3 stages: first it is ridiculed: then it is violently opposed: finally it is accepted as self evident. - Schopenhauer Posts: 5639 | From Aptos CA USA | Registered: Apr 2005
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