LymeNet Flash: new test method

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»	LymeNet Flash » Questions and Discussion » Medical Questions » new test method - gold standard?

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Topic: new test method - gold standard?

inglip
Member
Member # 14898

posted

Here is a promising new test:

Focus Floating
Microscopy
``Gold Standard'' for
Cutaneous Borreliosis?
American Journal of Clinical
Pathology 2007;127:213-222

+++++++++++++

A b s t r a c t
Borrelia burgdorferi is difficult to detect in routine
biopsy material from patients with skin lesions of
borreliosis. In this study, a new immunohistochemical
method, focus floating microscopy (FFM), was
developed to detect B burgdorferi in tissue sections and
was compared with polymerase chain reaction (PCR).
By using standard histologic equipment, tissue sections
stained with a polyclonal B burgdorferi antibody were
simultaneously scanned through 2 planes: horizontally
in serpentines and vertically by focusing through the
thickness of the section.
Borrelia were detected in 47 of 71 ticks, 34 of 66
tick bites, 30 of 32 erythema chronicum migrans cases,
41 of 43 borrelial lymphocytomas, and 50 of 51
acrodermatitis chronica atrophicans cases. FFM
proved to be more sensitive than PCR (96.0% vs
45.2%) and nearly equally specific (99.4% vs 100%).
All 169 control cases, except 1 false-positive case of
secondary syphilis, were negative with FFM. FFM is an
easy, quick, and inexpensive method to reliably detect
Borrelia in cutaneous tissue sections.
++++++++++++++++++++++++

Posts: 12 | From Sweden | Registered: Mar 2008 | IP: Logged |

seibertneurolyme
Frequent Contributor (5K+ posts)
Member # 6416

posted

Sounds promising if you are lucky enough to have a rash.

Bea Seibert

Posts: 7306 | From Martinsville,VA,USA | Registered: Oct 2004 | IP: Logged |

adamm
Unregistered

posted

Well, if it is indeed any good, you can be bloody sure that it'll never

know widespread use.

[Mad]

IP: Logged |

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