posted
I have conducted an experiment designed to test a hypothesis about the possibility of mast cells being the central cause of my symptoms. It proved to be a success, and I can now state it as scientific fact.
The mast cells I obtained from various sources, have same morphology, and are mucus subtype. They have been tested in vitro with {my} parasite, and the 'zoites effectively entered into live granulating cells, and survived.
It takes me to the next theory, which is; purposeful antigenic stimulation of these cells to enhance survival within host by parasite. What mechanisms are involved is unknown and speculative at this time.
I have stated that I DO NOT recommend anyone trying this, as there are some dangers associated with people who have a history of cardiac problems. Microw
-------------------- microw Posts: 129 | From Toronto, ON Canada | Registered: Feb 2008
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'Kete-tracker
Frequent Contributor (1K+ posts)
Member # 17189
posted
careful about making scientific factual statements, there, microw. D'you have a top biological lab you work in? Which one?? LOL
PSSST!: I have a few mast cells I'm unloading cheap... interested?
Posts: 1233 | From Dover, NH | Registered: Sep 2008
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may play a role in the development of POTS in some individuals. Some patients with orthostatic intolerance suffer from episodes of flushing, palpitations, shortness of breath, chest discomfort, headache, lightheadedness, hypotension or hypertension and occasionally syncope (Jacob & Biaggioni, 1999). Exercise may trigger an attack (Shibao, Arzubiaga, Roberts, Raj, Black, Harris & Biaggioni, 2005). Patients may complain of increased fatigue, sleepiness, increased urination and/or diarrhea after an attack (Jacob & Biaggioni, 1999). Symptoms of orthostatic intolerance often worsen after an episode. An increase in urinary methylhistamine, a marker of mast-cell activation, can be found in these patients.
Mast-cell activation results in the release of the vasodilator histamine, which may contribute to symptoms of POTS. Other mast cell mediators, such as plasma prostaglandin 2, may contribute to symptoms as well. Urinary histamine is often measured in the evaluation of flushing, but it is less specific than methylhistamine and not useful in the diagnosis of mast-cell activation (Shibao et al., 2005). Patients should be instructed to collect urine for a 4-hour period immediately after a severe spontaneous flushing episode. Urinary methylhistamine is usually normal between episodes in patients with mast-cell activation disorders, although the patients may experience chronic fatigue and orthostatic intolerance between episodes, which can lead to a disabling condition (Shibao et al., 2005).
Beta blockers should be used with caution, if at all, in those with mast-cell activation disorders (Shibao et al., 2005). Beta blockers may trigger mast-cell activation.
Posts: 789 | From CT, | Registered: Jun 2006
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bettyg
Unregistered
posted
al,
could you please break up your long, solid block post paragraphs into MANY SHORT paragraphs and double space between each one? big thanks; instructions below...
i'm interested in his post.
Welcome; i'm so glad you found us!! You've come to the right place for education and support!
Dr. Burrascano's most recent "Diagnostic Hints and 2008 Treatment Guidelines for Lyme and Other Tick Borne Illnesses" @
please go to my newbie links, copy the entire thing, and then print this off....FINANCIAL BURDENS compiled by melanie reber print off pages 74 - 92; outstanding info there. i believe there are a few more general comments there without links!! print that off too as it's newer info from members thru their own personal, tragic experiences.
Betty's suggested POSTING GUIDELINES . many of us have neuro lyme where we can NOT read long solid block text and be able to comprehend and read it as is.
please edit your post by CLICKING PAPER/PENCIL ICON to right of your name. that opens up BOTH subject line and body text.
now please break up your WORDY SENTENCES into one sentence paragraphs. Then hit ENTER KEY ``TWICE`` after each paragraph; we need that space for comprehension.
NOTE: you do NOT have to use " ", just show the name of person you are responding to, and then type your comment.
IF you need to use "", PLEASE DELETE "bold" codes so it's regular type text we read vs. the dark, harsh/painful bolding, delete the first 4 characters of 2ND LINE of a ""
[QB] just delete these 4 characters, and bolding is GONE! my eyes will really appreciate that; it's one very bad side effect of my having lyme for 38.5 years!! xox
then go to left hand corner and mark box to receive ALL REPLIES, and click EDIT SEND
we thank you for helping us; otherwise, we will SOB, SCROLL ON BY, since we can't read to help you. If I see posts like this, I SOB them; to hard on me. ------------------------------------------------------
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posted
Oxygenbabe, I have been wondering about that. It is probably why this thing is a disease in the first place. The macrophages do digest some, but many survive, and become viable once removed from prostate. The cysts are very tough. It might also have to do with class of macrophage
[maybe certain subtypes are restricted to prostate] because I would expect these cells to have some powerful enzymes including chitinases.
The cysts will "hatch" but the sporozoite contained within, rarely survives for long in extracellular fluid.
I have taken sporozoites from culture, and placed them with fresh prostatic fluid, and there was no activation of macrophages.
However, the sexual stage oocysts which are high in surface glycoprotein, immediately active surrounding cells. I believe the reason for oocysts being immunogenic, is for release of parasite to outside environment. Being in the intestine, they would stimulate lactoferrin, triggering diarrhea, thus releasing oocysts into environment to reinfect. This is rare occurrence hence the significant nature of this discovery.
The oocysts can withstand many chemicals including bleach dilutes.
The sporozoites and other phase elements do not cause reactions, but I think they are susceptible to antibodies. This might give them enough time in extracellular areas, to release spores which macrophages pick up, and the cycle is complete.
I assume they travel with macrophages to other areas of the body through lymph, but have no way of confirming that, except for the fact the same parasite exists in bowel system.
-------------------- microw Posts: 129 | From Toronto, ON Canada | Registered: Feb 2008
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posted
Al. The mast cells that are involved in my disease, are specific type. That is why it took so long to identify them. They almost resemble NK cells, but are bigger. I have a picture of on in my blog [see original post].
These cells are general maintenance cells found in mucus membranes. There is also a problem with sustained release of proteins from epithelial cells. They are not the type that would be activated by, say, a bee sting.
These cells are always present, and control bacteria, and other non threatening microbes. It is a "housekeeping" cell.
The continued antigenic stimulation by parasite, which has been demonstrated to invade these cells in vitro, causes slight amplification of mediators, resulting in some minor toxic effects. Minor inflammatory reactions are also present, but not enough to alarm other immune factors. This is a nuisance disease, and would not cause enough problems to warrant medical investigation. This is why I have taken matters into my own hands.
My research can be helpful to understand more about the behavior of other intracellular parasites, by observing the high success rate of this species. It makes it easy for a researcher, when he is the lab rat!
-------------------- microw Posts: 129 | From Toronto, ON Canada | Registered: Feb 2008
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otherwise, we will SOB, SCROLL ON BY, since we can't read to help you. If I see posts like this, I SOB them; to hard on me. 
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