posted
Band 41 can be positive from anything with flagella. I know ecoli is one that can cause it but there are a ton of others too. I know I've heard that epstein barr causes a couple bands to be positive too but I'm not sure which ones.
Posts: 499 | From Indiana | Registered: Oct 2007
| IP: Logged |
CherylSue
Frequent Contributor (1K+ posts)
Member # 13077
posted
I've heard that some viruses can make some bands positive, but it wasn't the main bands that we react to, it was some lesser bands I recall. I can't find the reference, but I'll update you when I find it. It may have been in Ken Singleton's book.
Posts: 1954 | From Illinois | Registered: Aug 2007
| IP: Logged |
randibear
Honored Contributor (10K+ posts)
Member # 11290
posted
what about chronic fatigue or systemic yeast? would they do it?
-------------------- do not look back when the only course is forward Posts: 12262 | From texas | Registered: Mar 2007
| IP: Logged |
posted
Chronic fatigue is a symptom, don't ever let a doctor try and tell you it's a diagnosis. That would be like you going to a doctor and saying "I've had this horrible headache, tinnitus and blurred vision, what is this" and he just says "oh it's chronic headache syndrome, pay my secretary and come back in two weeks"
Posts: 499 | From Indiana | Registered: Oct 2007
| IP: Logged |
Shosty
Unregistered
posted
Lupus can cause a false positive Elisa Lyme test.
I tried for years to research your question. I did find, in one place, that the 23-25 Kda band could be a false positive due to autoimmunity, but have not found that again.
IP: Logged |
disturbedme
Frequent Contributor (1K+ posts)
Member # 12346
posted
Ah, I don't know if I'd totally believe Lupus causing a false positive ELISA lyme test!!! For one thing, many LLMDs believe lupus is really just lyme... or that lyme caused the lupus in the first place. As many LLMDs believe that lyme is what causes most, if not all, autoimmune issues.
-------------------- One can never consent to creep when one feels an impulse to soar. ~ Helen Keller
My Lyme Story Posts: 2965 | From Land of Confusion (bitten in KS, moved to PA, now living in MD) | Registered: Jun 2007
| IP: Logged |
nenet
Frequent Contributor (1K+ posts)
Member # 13174
posted
I believe it is 31 that can cross react with Eppstein Barr virus (not sure of other viruses).
If band 31 is the only Lyme significant band that shows up on a test, you can get a test from IGeneX that will determine whether it is from Lyme or EBV.
Someone please correct me if I am wrong. There are a lot of threads about this topic here. It would be a good idea to search for:
TerryK
Frequent Contributor (5K+ posts)
Member # 8552
posted
Per an ILADS doctor
Besides 41,
Band 31 HIV Hepatitis Epstien Barr
Terry
Posts: 6286 | From Oregon | Registered: Jan 2006
| IP: Logged |
timaca
Frequent Contributor (1K+ posts)
Member # 6911
posted
My understanding is that other pathogens, including other borrelias can yield positive bands on a WB test. That is why the CDC set 5 out of 10 bands on the IgG WB , so that you are making sure it is Bb that is the problem not some other pathogen.
So, the fewer bands one has, the less likely it is lyme.
You are trying to find the correct answer, not just any answer...and certainly not a wrong answer.
Best, Timaca
Posts: 2872 | From above 7,000 ft in a pine forest | Registered: Feb 2005
| IP: Logged |
TerryK
Frequent Contributor (5K+ posts)
Member # 8552
posted
1: Infection. 1992 Sep-Oct;20(5):283-6
Relapsing fever and its serological discrimination from Lyme borreliosis.Rath PM, R�gler G, Sch�nberg A, Pohle HD, Fehrenbach FJ.
Abt. f�r Medizinische Mikrobiologie, Universit�tsklinikum Rudolf Virchow, Germany.
Patients with Borrelia-caused relapsing fever produce cross-reacting antibodies to Borrelia burgdorferi, the anti-genetically related causative agent of Lyme borreliosis.
The antibody response of the serum of a patient (acute and convalescent) with relapsing fever was analysed by the immunoblot technique using Borrelia hermsii and B. burgdorferi as antigens. The diagnosis was established by microscopic detection of spirochetes in the patient's blood.
The patient's serum showed significantly elevated titers of IgG and IgM in a B. burgdorferi indirect immunofluorescence assay. Immunoblot analysis indicated the presence of cross-reacting antibodies directed to B. burgdorferi antigens with apparent molecular weights of 60, 41, 40, 36, 30 and 20 kDa.
PMID: 1385332 [PubMed - indexed for MEDLINE]
Posts: 6286 | From Oregon | Registered: Jan 2006
| IP: Logged |
TerryK
Frequent Contributor (5K+ posts)
Member # 8552
[Diagnosis of Lyme borreliosis with western blotting][Article in Czech]
Hul�nsk� D. N�rodn� referencn� laborator pro lymeskou boreli�zu, St�tn� zdravotn� �stav, Praha.
Spirochetes Borrelia burgdorferi sensu lato, selected as antigens for Western blot analyses were isolated from cerebrospinal (strain 192 M) and from blood (strain Kc90) and identified by means of monoclonal antibodies and the polymerase chain reaction (PCR) as B. garinii and B. afzelii.
Differences between B. garinii and B. afzelii are in the genotype of the surface protein OspA and OspB, internal flagellin (Fla II) and the main extracellular protein (MEP). The reaction of polyclonal antibodies in 918 serum specimens and 180 specimens of cerebrospinal fluid was investigated in IgG and IgM immunoblots in patients with neurological symptoms, arthritis and skin manifestations suspect of Lyme borreliosis.
Confirmation of the immunoenzyme ELISA reaction by means of immunoblots in the acute stage of borreliosis, in clinically obscure cases, in herpetic infection, mononucleosis and leptospirosis revealed a higher sensitivity and specificity of Western blot.
Proteins of B. garinii with a molecular weight of 94, 84, 66, 60, 56, 41, 39, 33, 29, 22, 18 and 14 kDa were detected in the reaction with monoclonal antibodies and immunoglobulins of patients suffering from barreliosis.
The frequency and intensity of the reaction of these antigens differed markedly in sera of patients suffering from borreliosis and sera of patients who suffered from a different infection.
The external surface antigen OspA, OspB, OspC and protein with 39 kDA are significant markers of borreliosis.
The most frequently detected antigens in cross reactions with immunoglobulins against other pathogens are proteins P66, P60, P41 which are dominant immunogens of all types of borrelias and moreover a humoral response to them develops in the acute stage of the disease. In arthritis and neuroborreliosis a different in IgG immunoblots was found.
PMID: 9162453 [PubMed - indexed for MEDLINE]
Posts: 6286 | From Oregon | Registered: Jan 2006
| IP: Logged |
TerryK
Frequent Contributor (5K+ posts)
Member # 8552
Immunodiagnosis of human granulocytic ehrlichiosis by using culture-derived human isolates.
Ravyn MD, Goodman JL, Kodner CB, Westad DK, Coleman LA, Engstrom SM, Nelson CM, Johnson RC.
Department of Microbiology, University of Minnesota Academic Health Center, Minneapolis 55455-0312, USA. [email protected]
Human granulocytic ehrlichiosis (HGE) is an emerging infection caused by an Ehrlichia species closely related to Ehrlichia equi and Ehrlichia phagocytophila.
Recent advances in the isolation and cultivation of this organism have allowed us to develop an immunofluorescence assay (IFA), enzyme immunoassay (EIA), and Western immunoblotting (WB) using HL-60 cell culture-derived human isolates.
Antibody was detected in sera from culture-confirmed HGE patients by IFA and EIA, and these samples were reactive when analyzed by immunoblot analysis.
HGE patient sera had high antibody titers and did not react with uninfected HL-60 cells. When IFA, EIA, and WB were used to analyze sera from healthy donors or those with a range of other disorders, including infections caused by Ehrlichia chaffeensis, Rickettsia rickettsii, and Coxiella burnetti, no significant cross-reactivity could be detected by EIA or immunoblot analysis with the exception of two of four serum samples from R. rickettsii-infected patients that were reactive by IFA only.
Sera from HGE patients did not significantly cross-react in serologic tests for Borrelia burgdorferi. Using sera from patients previously enrolled in two clinical trials of treatment for early Lyme disease, we evaluated a two-step approach for estimation of the seroprevalence of antibodies reactive with the etiologic agent of HGE.
On the basis of the immunoblot assay results for sera from culture-confirmed HGE patients, WB was used to confirm the specificity of the antibody detected by EIA and IFA. EIA was found to be superior to IFA in the ability to detect WB-confirmed antibodies to the HGE agent.
When EIA and WB were used, 56 (19.9%) patients with early Lyme disease (n = 281) had either specific immunoglobulin M (IgM) or IgG antibodies; 38 patients (13.5%) had IgM only, 6 (2.1%) had IgG only, and 12 (4.3%) had both IgM and IgG. Therefore, Lyme disease patients are at high potential risk for exposure to Ehrlichia.
Analysis by immunoblotting of serial samples from persons with culture-confirmed HGE or patients with Lyme disease and antibodies to the agent of HGE revealed a reproducible pattern of the immune response to specific antigens.
These samples confirmed the importance of the 42- to 45-kDa antigens as early, persistent, and specific markers of HGE infection. Other significant immunogenic proteins appear at 20, 21, 28, 30, and 60 kDa.
Use of the two-test method of screening by EIA and confirming the specificity by WB appears to offer a sound approach to the clinical immunodiagnosis of HGE.
PMID: 9620365 [PubMed - indexed for MEDLINE] PMCID:
Posts: 6286 | From Oregon | Registered: Jan 2006
| IP: Logged |
TerryK
Frequent Contributor (5K+ posts)
Member # 8552
Bruckbauer HR, Preac-Mursic V, Fuchs R, Wilske B. Max von Pettenkofer Institute, University of Munich, Germany.
The specificity of serological tests for Lyme borreliosis is impaired by cross-reacting antibodies.
In order to select antigens for more specific tests, specific and cross-reactive proteins of Borrelia burgdorferi must be identified.
Therefore, to analyze cross reactions of Borrelia burgdorferi with other bacteria, rabbit immune sera against heterologous bacteria (Borrelia hermsii, Treponema pallidum, Treponema phagedenis, Leptospira interrogans (serogroup grippotyphosa), Neisseria meningitidis, Haemophilus influenzae, Yersinia enterocolitica (serotypes O3 and O9), Campylobacter jejuni, Listeria monocytogenes O1, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium, Shigella flexneri and Legionella micdadei) were examined by Western blot using Borrelia burgdorferi as antigen.
Broad cross reactivity was shown for Borrelia proteins of the 60-75 kDa range. Other broadly cross-reacting proteins were at the level of p40, p33 and two proteins in the range of 20 kDa.
Some of the cross reactions were eliminated by absorption of the sera with Treponema phagedenis. The absorbed antibodies were directed mainly against bands at the level of p33 and bands of the 60 to 75 kDa range.
Showing the lowest potential for cross reactivity, p100, p41, OspA and pC seem to be the most suitable antigens for serodiagnosis.
In contrast to p100 and OspA, however, p41 and pC showed cross reactivity with immune sera against bacteria not belonging to the genus Borrelia.
PMID: 1597198 [PubMed - indexed for MEDLINE]
Posts: 6286 | From Oregon | Registered: Jan 2006
| IP: Logged |
quote:Originally posted by disturbedme: [QB] Ah, I don't know if I'd totally believe Lupus causing a false positive ELISA lyme test!!! For one thing, many LLMDs believe lupus is really just lyme... or that lyme caused the lupus in the first place. As many LLMDs believe that lyme is what causes most, if not all, autoimmune issues.
Amen to everything you said!
-------------------- --Lymetutu-- Opinions, not medical advice! Posts: 96239 | From Texas | Registered: Feb 2001
| IP: Logged |
The Lyme Disease Network is a non-profit organization funded by individual donations. If you would like to support the Network and the LymeNet system of Web services, please send your donations to:
The
Lyme Disease Network of New Jersey 907 Pebble Creek Court,
Pennington,
NJ08534USA http://www.lymenet.org/
UBBFriend: Email this page to someone!
Printer-friendly view of this topic