posted
Forskolin's connection with GABA got my attention. Remind me, what's the connection between forskolin and Frontline?
Posts: 727 | From USA | Registered: Mar 2006
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posted
non sequitur . So there is some obscure cAMP regulation (one of many other pathways its regulated) ,then you somehow jump to "Frontline" (wtf is that) and then you jump to a OspB antibody
Posts: 856 | From MA | Registered: Jul 2009
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
dguy:
Chloride channels...if inhibited (via Frontline for our DOGS) = lyme is not transmitted.
"In the case of the fipronil/(S)-methoprene combination product (FRONTLINE Plus)"
Fipronil Mechanism of Action
Fipronil (a phenylpyrazole compound) causes hyperstimulation of muscle neurons in invertebrates by
***blocking passage of chloride ions in γ-amino butyric acid (GABA) mediated chloride channels.***
Hyperexcitement and death of adult fleas and all life stages of ticks result on contact. The same principle applies to chewing lice and sarcoptic mites."
It is my understanding that GABA A and GABA C are chloride channels, NOT GABA B.
GABA comes FROM glutamate.
GABA A and GABA C (chloride) = FAST inhibition.
GABA B = SLOW inhibition.
Once infected, those chloride channels are "active".
See also my additional info. under the Nicotinamide post re: forskolin.
This all looks to relate to burning fat -> ketones. Aren't we all being told to get skinny? Latest "news" says obesity causes cancer...
Nahhh...we're just feeding glucose - loving - pathogens which trigger excessive ROS to be destroyed -> excessive inflammation -> DNA - ROS damage = "hello cancer".
We are overstressing our immune systems.
Tetracycline does contain 2 ketones...
Our brain can use ketones as an alternative fuel (instead of glucose), but can glucose loving/needing pathogens?
Our bodies downregulate Bb's OspA via lowering dopamine -> norepinephrine (which Bb uses). Though sometimes it can "re-emerge" as an outer surface protein.
The anti-oxidant, melatonin, lowers dopamine levels.
Once infected USUALLY OspA is downregulated...leaving OspB to contend with, but our own antibodies to that outer surface protein, (mAB CB2) are "damaged" and don't work.
Here we go again:
Characterization of the physiological requirements for the bactericidal effects of a monoclonal ***antibody to OspB*** of Borrelia burgdorferi by confocal microscopy.
The bactericidal effect of Fab-CB2 is not dependent on the induction of spirochetal proteases but
***is dependent on the presence of Ca2+ and Mg2+.***
Supplementation of Ca2(+)- and Mg2(+)-free medium with these cations
***restored the bactericidal effects of Fab-CB2.***
The mechanism by which a Fab fragment of an antibody destroys a bacterium directly may represent a novel form of antibody-organism interaction.
PMID: 9125579
A ``novel form of antibody-organism interaction?'' I don't THINK so!
E. Required by immunological process. Magnesium, immunity, and allergy: Mg is required for several steps of immunological reactions
1. Lymphoblastic transformation, a prerequisite of secretion of antibodies by lymphoblasts, requires Ca2+ and Mg2+
2. Mg is required for synthesis of proteins, immunoglobulins included
3. Antibody-induced complement activation is Mg dependent
4. The antigen-immunoglobulin-complement reaction induces degranulation of the mastocyte
At the outset of lyme, our Mg levels drop "significantly".
And this continues to spiral down as it is very hard (almost impossible) to restore the amt. of MgCl (with B vitamins...esp P5P) that is needed. Instead of MgCl going into the cells, more "reactive" NaCl and CaCl go in...and then out...to Bb's "happiness" and "health"...
Bb uses NaCl for motility and looks to exchange H for Na. So long hydrogen.
The Romanian doctors did it...they restored Mg levels WHILE giving IV abx. to cure lyme (albeit only 3 patients and early onset).
The "cure" looks to be 2 fold - reduce inflammation (TNF alpha and IL1 B) WHILE hitting the pathogen.
Mg is an anti-inflammatory and anti-histamine.
There IS more than one way to do the above.
It might be important if using forskolin (to breakdown fats) to ALSO take something to reduce TNF alpha and IL1 B, such as OmegaBrite for example. Pycnogenol is another possibility.
Once infected, even IF the infection is cleared, are the same proteins, genes, expressed? Are they stuck "on"?
Can we downregulate those protein/genes? Or take the essential nutrients to restore others (tumor *suppressor* genes)?
Coltman...we're going back to the "beginning"...how do we prevent lyme (in dogs)...which has to do with blocking chloride channels and by doing so, this appears only allow GABA B (slow inhibition, not a chloride channel) to work.
The GABA B receptors:
"They can stimulate the opening of K+ channels which brings the neuron closer to the equilibrium potential of K+, hyperpolarising the neuron.
***This prevents sodium channels from opening,*** action potentials from firing, and VDCCs from opening, and so stops neurotransmitter release."
Oddly they also
***"REDUCE the activity of adenylyl cyclase*** and decrease the cell's conductance to Ca2
Adenylyl cyclase helps to do this:
ATP → AMP + PPi
In contrast, forskolin INCREASES adenylyl cyclase -> more pyrophosphate "available".
It appears Bb is "robbing" us of 2 phosphates.
An Italian doctor named Valletta has a U.S. patent titled: Magnesium for Autoimmune.
He used Mg PYROPHOSPHATE and sub (lingual) B6 to cure several diseases...
He did "jumpstart" the healing via IV...
Mg INactivates HMG CoA reductase and halts the cholesterol pathway too...
If we can keep levels high enough, long enough and not let Bb rebuild "his" cell walls, then step #2 can kick in to destroy i.e., "finish off" the CWD form via osmotic pressure changes/ultrasound.
Those are the steps needed to destroy gram negative pathogens...first destroy the cell wall/prevent its formation and then finish it off via osmotic pressure changes or ultrasound.
I suspect barometric pressure changes (which impact O2 levels) also help out.
Mother Nature to the rescue...so to speak.
Bet you ache more when storms approach. Yes?
[ 09-24-2009, 03:17 PM: Message edited by: Marnie ]
Posts: 9424 | From Sunshine State | Registered: Mar 2001
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cantgiveupyet
Frequent Contributor (1K+ posts)
Member # 8165
posted
Marnie, Is this the same as mentioned in the Duke study in the below link? They found this helpful to break down biofilms in the bladder
If this is not the same, please let me know and i will remove this post.
-------------------- "Say it straight simple and with a smile."
"Thus the task is, not so much to see what no one has seen yet, But to think what nobody has thought yet, About what everybody sees."
-Schopenhauer
pos babs, bart, igenex WB igm/igg Posts: 3156 | From Lyme limbo | Registered: Oct 2005
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Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
YUP...see my additional post today re: forskolin and MANGANESE...which Bb NEEDS.
Posts: 9424 | From Sunshine State | Registered: Mar 2001
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