LymeNet Flash: Where To Find Darkfield Microscopy in the US?

This is topic Where To Find Darkfield Microscopy in the US? in forum Medical Questions at LymeNet Flash.

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https://flash.lymenet.org/ubb/ultimatebb.php/topic/1/134343

Posted by TexasBartonella (Member # 47977) on :

Does anyone know of a lab or person in the US that does darkfield microscopy for a fee if blood is shipped to them? Thanks!

Posted by bluelyme (Member # 47170) on :

Of the known ones ther is fry labs and galaxy...but Yes pm me ....one may be near you,one may not be in us but his associate is ..

Posted by Harmony (Member # 32424) on :

you can buy the microscopes on Amazon, if all else fails and learn to do the slide prep yourself (it is not easy)

https://www.amazon.com/gp/product/B00E00K7CC/ref=oh_aui_search_detailpage?ie=UTF8&psc=1

get the digital camera and take pictures to your doc [hi]

https://www.amazon.com/gp/product/B004WAGOKY/ref=oh_aui_search_detailpage?ie=UTF8&psc=1

it's not easy, but it is a possibility

Posted by bluelyme (Member # 47170) on :

There is a chiro in odessa ,has one, and here in nm he will send out and identify..and i think mexico clinic has one ...

'dude from ks 'is using a amscope and making his own cultures ...like iv abx its a steep learning curve ...you can see quite a bit with lightfield..its how i confirmed spirochettes. .

Posted by Harmony (Member # 32424) on :

bluelyme, how did you do the slide prep to see the bacteria?

very curious to know [bow]

Posted by Lymedin2010 (Member # 34322) on :

http://www.phmiracleliving.com/t-microscopist-list.aspx

Posted by bluelyme (Member # 47170) on :

Lymed has some u toob vidies under borrelia tamer...some coat the edge with imersion oil ...clean area in side of finger ,use a diabetic pricker .wipe first drop away ..get in middle of cover slip .,2 per slide ...i crush rbc with light pressure from bic pen (plastic end )

I just did my first giemsa stain using a 3 part vet dye used to test cattle for babs...they sent it right away..that was easy too..drya thick and thin smear , dip in each then rinse with distilled ..pat dry with chem wipe ..oil on smear no cover slip

read microscopy thread ..lymed ,dude and tnt are the experts ..its awesome yet scary cool..i am using friends brightfield 1000x oil ....

Posted by Harmony (Member # 32424) on :

wow - you all are the best!

I have the Amscope but find it hard to see things and then interpret what I see

I will use your tips and try again, when I have a chance

Posted by lymenotlite (Member # 33166) on :

Anyone know what happened to the Microscopy Thread?

Posted by me (Member # 45475) on :

Here is one link:

http://flash.lymenet.org/ubb/ultimatebb.php/topic/1/120458#000000

If you go to the "search" function, you can type in search terms and select the forums you want to search in. It will pull up threads.

Posted by me (Member # 45475) on :

I've searched on the Internet about how to prep blood with slides and look for spirochetes using dark field microscopy. I can't find what I'm looking for.

Does anyone have a link with step by step instructions, along with corresponding visuals, to do this?

Posted by Lymedin2010 (Member # 34322) on :

https://www.youtube.com/watch?v=IZ1scQdbmtg

https://www.youtube.com/watch?v=kFQom3ssd38

Posted by me (Member # 45475) on :

Thank you, Lymein2010!