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» LymeNet Flash » Questions and Discussion » Medical Questions » Lipid Exchange between Bb and Host Cells.

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Author Topic: Lipid Exchange between Bb and Host Cells.
Pinelady
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Lipid Exchange between Borrelia burgdorferi and Host Cells.
http://www.ncbi.nlm.nih.gov/pubmed/23326230
LoS Pathog. 2013 Jan;9(1):e1003109. doi: 10.1371/journal.ppat.1003109. Epub 2013 Jan 10.

Crowley JT, Toledo AM, Larocca TJ, Coleman JL, London E, Benach JL.
Source

Department of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, United States of America.
Abstract

Borrelia burgdorferi, the agent of Lyme disease, has cholesterol and cholesterol-glycolipids that are essential for bacterial fitness,

are antigenic,

and could be important in mediating interactions with cells of the eukaryotic host.

We show that the spirochetes can acquire cholesterol from plasma membranes of epithelial cells.

In addition, through fluorescent and confocal microscopy combined with biochemical approaches,

we demonstrated that B. burgdorferi labeled with the fluorescent cholesterol analog BODIPY-cholesterol or

(3)H-labeled cholesterol

transfer both cholesterol and cholesterol-glycolipids

to HeLa cells.

The transfer occurs through two different mechanisms,

by direct contact between the bacteria

and eukaryotic cell

and/or through release of outer membrane vesicles.

Thus, two-way lipid exchange between spirochetes and host cells can occur.

This lipid exchange could be an important process that contributes to the pathogenesis of Lyme disease.
-----------
Whooo HOooo!!!

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
Pinelady
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We needed this research on many fronts.
Ya know all those cholesterol plagues and calcium deposits in arteries they never alluded to a cause other than you's just fat....Nope.

All those tiny calcium deposits that herald most breast cancers? Yep.

Infected cell lines used in vaccine made with HeLa cells contaminated with spirochetes? Yep.

Ghost cells associated with prion protein infections. Yep.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
VV
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Not quite sure I understand. Is this helpful in devising a new treatment?
Posts: 922 | From Philadelphia | Registered: Sep 2012  |  IP: Logged | Report this post to a Moderator
seibertneurolyme
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I interpret this differently than Pinelady.

I think it means that borrelia needs cholesterol and robs it from the body.

Babesia has been proven to rob the good fats -- phosphatidylcholine -- and I wouldn't be surprised if borrelia does not do the same. Don't have time to look up that pubmed article right now.

That is one of the reasons for nerve damage -- one of the main components of the myelin sheath is phosphatidylcholine I think.

Bea Seibert

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Pinelady
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The transfer occurs through two different mechanisms,

by direct contact between the bacteria

and eukaryotic cell

and/or through release of outer membrane vesicles.
--------------------------
Bea---it means that the vaccines that used HeLa cells from a woman who had Syphilis were most likely infective. That makes them responsible.

http://www.youtube.com/watch?v=ubSnxCr7kz8&feature=share

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
Pinelady
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Multi-locus typing scheme for Babesia bovis and Babesia bigemina reveals high levels of genetic variability in strains from Northern Argentina.

http://www.ncbi.nlm.nih.gov/pubmed/23287026

It means they continue to screw up big time because of the genetic variance seen in borreli/hiv is not the only place it has infected.
It means vaccines are done.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
VV
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"It means vaccines are done."

I still don't understand.

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nefferdun
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I have trouble following scientific explanations but PR multiplies 100 times faster with fats. So borellia likes fats too?

I have been staying off drugs using liposomal phosphatidylcholine with EDTA to degrade the biofilm.

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old joke: idiopathic means the patient is pathological and the the doctor is an idiot

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VV
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I take 2 tablespoons of soy lecithin a day. Does that fit in here somewhere?
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Pinelady
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I would Plant sourced vitamin E instead of GM soy.

Vitamin E can reduce cholesterol.

But you don't want to reduce cholesterol unless your killing bugs.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
Pinelady
Frequent Contributor (5K+ posts)
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HeLa cells came from a woman who had spirochetal disease.

Henrietta Lacks immortal cells used in vaccines came from a woman who had Syphilis.

They were infective not protective. That is why they were immortal.

That is where the real cause of AIDS came from. Not HIV.

That is why Prof. Montagnier can find spirochetes in 80% of the pop.s.
http://www.youtube.com/watch?v=ubSnxCr7kz8&feature=share

Then when they added to that mix by giving Lyme Vaccines to the people, the pets, and our feed animals ---

They added to the immune suppression and subsequent seronegative tests.

--------------------
Suspected Lyme 07 Test neg One band migrating in IgG region
unable to identify.Igenex Jan.09IFA titer 1:40 IND
IgM neg pos
31 +++ 34 IND 39 IND 41 IND 83-93 +
DX:Neuroborreliosis

Posts: 5850 | From Kentucky | Registered: Dec 2008  |  IP: Logged | Report this post to a Moderator
   

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