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Originally posted by Catgirl:
http://www.medsci.org/v10p0362.htm

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Posts: 786 | From UK | Registered: Oct 2007  |  IP: Logged | 

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Originally posted by lhctom:
I'm no CDC fan but its troubling that 29 patients from across the US were found to have the garinii species not found in the US and at least 21 had the exact same 603 letter pyrG gene that also matched the Japanese JujiP2 garinii strain that was acquired by ALS and used to test the culture.

The odds of finding so high a percentage of garinii infections in the US is quite low. The odds of spirochetes from multiple US sites having the exact same 603 letters in their pyrG gene is even lower. To have 21 matches to the 603 letters of the ATCC test FujiP2 isolate is very low. The odds all three would happen is the product of the three very low odds. This is not good.

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Originally posted by susank:
lhctom: could you copy and post your sequencing results? i never did that part of the test and have been curious about what the results would look like. thanks.

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Originally posted by lhctom:
Below is the actual quote by Nick Harris from IGenex.

Lisa aka Elena, would have you believe the test is 10% sensitive. But she is conveniently leaving off most of what Nick Harris actually said. The test has been shown to be 75-90% (other studies have shown 96%) except late stage infections where a small study at IGenex showed only 10%. Since this is the only other test offered in the US that is recognized by ID doctors, if you want to get a diagnosis that is believed, the C6 is one way to try. Its very dishonest to partially quote someone or a study just to give the impression you want. We all know this is exactly what the IDSA does. So isn't this the same? Lisa, Elena or whoever she is is giving out information intentionally altered from a direct quote. This is exactly the anti-science behavior that has led to all the problems. We need honest activists if we want the truth to emerge. I said the C6 has problems but we are very limited to accepted and validated tests here in the US. Igenex offers the test. I have late stage Lyme and I was positive at IGenex and tony Brook on the C6. It helped me. Why would IGenex offer the test if Nick Harris did not see some value?

Direct quote Nick Harris

http://www.ldsg.org/index.php?id=11&no_cache=1&tx_ttnews[tt_news]=57&tx_ttnews[backPid]=4&cHash=ab5f39f8e9

3) The ELISA test and C6 ELISA test:

The ELISA test works quite well for patients with EM rash and a known tick bite, within 3-4 months of the tick bite. After 3-4 months, the test loses sensitivity. The C6 peptide ELISA detects the C6 found within the antibody formed against borrelia, so this is an antibody test. In a study with patients with CDC-positive IgG and IgM Western blot tests, the C6 ELISA was positive in 73% of these patients. In a set of 30 patients with late Lyme disease, all having CDC-positive IgG and IgM Western Blots, the C6 ELISA was positive in only 10% of the patients. It is not a good test for late-stage Lyme disease. Other studies have been done with various results, all with 100% of patients CDC-positive by Western Blot. The best result for the C6 ELISA was just under 90% - results varied widely from study to study.

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Originally posted by Shadrach:
Dear Ed,
Have You ever used CLUSTAL X?

Try It.
You will find detail that is present in BLASTn
but easier on the Eye.

22% of Sapi's Garinii-like isolates had either one
or two base substitutions with
reference strain AB55778.1 haplotype (n==603)
Garinii.
With Barcelona haplotypes (n=603)in GenBank
Jf331248.1 Jf331231.1, and
Jf331265.1 Japanese haplotype
the identity among this reference group of 4 was 100 each with each other. Net take home message:
all bona fide Garinii haplotypes, but difference by 166 base mismatches with the Sapi GenBank Deposits.
Have you ever successfully made a GenBank deposit
which passed muster and was published by Genbank?
I would venture a guess that you have not.

So make the move over to Clustal analysis for DNA sequence alignments. It works!
By the way in B31 the complete pyrG sequence is 1602 bases,-- Soooo with 603 bases to examine we are only seeing a part of th total pyrG ORF
Alan MacDonald MD , FCAP FASCP
Aug 25 2013
PS:It is now Aug 25 2013 at 14:38 hours.
Do you know where your CDC deposits Kf170280
KF170281 , KF170282 are??
They are not presently available to the Public.
Perhaps missing in action/
Perhaps prisoners in a CDC Remote site with Cheney.
Perhaps they will never see the clear light of day.

With No CDC GeneBank Deposits available to the public,
How is it that the Outside expert reviewers
were able to complete their review of the CDC paper [ without the missing 3 DNA CDC reference pyrG sequences? ]
Did the paper bypass Outside Impartial review? Who paid between $2000 to $3000 for the CDC paper to have accelerated paper-less publication { epub ahead of print}??
Were taxpayer funds used to advance a CDC false scenario?
Alan b. MacDonald MD

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Originally posted by susank:
Poppy - its all over my head but was having the same thoughts.

I have not studied Bgar - but the bit I did read about - seems Bgar found in colder climates.
Saw a ref to Alaska?

Dunno - does Igenex test for Bgar?

Wonder why ALS would? Of course people travel to other countries and could be exposed. And folks move here from the endemic countries.

It gave me a bit of a scare in regards to popssible problems with the ALS culture - else I would not be in on this discussion.

I feel confident my Bb pos. test from ALS was not compromised.

Its just all weird about the Bgar and Baf.

To add - re: Elena?s questions to me:

I "believe Abx cause false positive WB's"????
then
I "believe Abx cause positive WB's" ???

I thought I might have still a few marbles left in my head - Abx are taken pre-test to "help" get the pathogens out/into the blood - to be detected on a WB? Right?

No one wants a positive test. But if you are Bb positive you sure want one. Instead of wandering in the wilderness wondering what the "h" is wrong with you.

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Originally posted by Shadrach:
Call for CDC Freedom of Information Release of
Documents relevant to the Johhnson et al
CDC official critique of Advanced Labs Blood human blood culture patient results:
________________________________________________
Dear Dr. MacDonald,
                All FOIA requests must be submitted formally to the CDC FOIA office. More information can be obtained at the following website: http://www.cdc.gov/od/foia/
 
 
Sincerely,
 
 
 
***************************************************
C. Ben Beard, Ph.D.
Chief, Bacterial Diseases Branch
Division of Vector-Borne Diseases
CDC
Mail Stop P-02
3156 Rampart Road
Fort Collins, CO 80521 USA

 
From: alan macdonald [mailto:[email protected]]
Sent: Monday, August 26, 2013 5:05 PM
To: Beard, Charles B. (CDC/OID/NCEZID)
Subject: Request for documents under the freedom of information act
 
Dear Dr. Beard,
 
As the official CDC spokesperson for all communications relating to Lyme borReliosis
from US Citizens:
 
I respectfully request that you provIDE me with any and all
communications which were received by the CDC from :
 
1.State Public Health Officials
2. PHYSICIANS
3. Scientists with US Governmental funding for Lyme Borreliosis Research
4. Federal Employees
5. Members of the U.s. Congress and the U.S. Senate
 
In connection with allegations that  flawed Medical  Diagnostic
services were rendered to the public by
Advanced Laboratories, a fully licensed and fully compliant 
medical  diagnostic facility under the Statues of Pennsylvania.
 
Under the Freedom Of Information Act, I am requesting documentation
for the allegations of .....Bad Laboratory Medical diagnostic Practices .....(SIC) 
by  Advanced  Diagnostic Laboratories, as is presentED in the
CDC Sanctioned publication in Journal of Clinical Microbiology
2013, "Assessment of New Culture Method to Detect Borrelia species
in Serum of Lyme Disease Patients", 2013, 14 August, e Pub ahead of print
J.Clin. Microbiology, doi:10.1128/JCM.01674-13, authored by
"Barbara J. B.Johnson, Mark A. Pilgard, and Theresa M Russell"
"Division of Vector-Borne  Diseases, Centers for Disease Control and Prevention,
Ft. Collin, Co, USA"
 
Direct quotationS from the published manuscript of Johnson et al as captioned
above: {Begin Quote]
 
"    The Centers for Disease Control and Prevention has received numerous  inquiries  From
national and state health departments and clinicians about the performance of this
culture method since Advanced Laboratory Services began  offering  it in 2012
[http://www.advanced-lab.com/spirochete.php ].."   End quote] { manuscript submission line 64-67]
 
 
Further:  Johnson et. al. state in their published manuscript:
 
   [Line 151-152]   Begin quote
"The methods used by Sapi et. al. to assess test specificity were inadequate
to rule out false positivity by culture of Laboratory strains of PCR amplicons"
[Line 195-204}
 
" ...taken together ,our data and those of Sapi et.al. indicate that laboratory
contamination  was the probable source of the Borrelia DNA found in the patient 
specimens. The vast majority of patient pyrG sequences (41/51) are indistinguishable
from laboratory strains used by the investigators. the clinical relevance of the
of the pygR sequences (10/51) is unclear; these findings may also be consistent
with laboratory  contamination. Before use in Clinical medicine, novel findings must
be independently verified. Independent verification is particularly critical when
claims are at  odds  with a large body of other scientific work, and when they may trigger 
unnecessary antibiotic treatment of patients. We caution clinicians and patients to
wait for independent verification by scientifically sound methods before using this
culture service for diagnostic services"   End quote
 
The contamination scenario described by Johnson and CDC employees is IMPOSSIBLE.
because the Live Control ATCC strains used to validate the acceptability of
a modified BSK Media
were all done in the West Haven research laboratory .
Advanced Laboratories of Philadelphia and were NEvER
in psooession of live Control borrelia.
Summation:
TWO  geographially  separated Laboratories -  in a joint scientific Study -
No Cross contamination between the two laboratories.
 
This single fact Negates the Entire content of the Johnson et al CDC authored
J. Clin.  Microbiology   publication.
 
I have already notified Dr. Thomas Frieden  CDC Director of these facts.
I have received no response from Dr Frieden  as of this  date.
 
The Johnson et al publication must be officially Retracted.
It is Factually Flawed.
 
It leads the reader to believe that CDC deposits to GenBank
were  published  in advance of the J. Clin  Microbiology  article 
so that Impartial and highly vetted expert outside reviewers could analyze
.the content of DNA sequences  { generated by CDC scientists in Ft Collins, Co,}
 
[ KF 170280, KF 170281, KF 170282 ]
 with 
and use Clustal analysis and BLASTn analysis  to compare these with GenBank 
accepted and published  deposits  ofborrel Isolates from humans Subject: CDC abuse of Power - OpEdto the Washington Post
 
Summary: CDC Misuse of Power
------------------------------------------------------------------------------------------------------------------------------------
A crucial ERROR in the CDC paper doi:10.1128/JCM.01674-13 was the CDC assumption that CONTAMINATION of patient blood 
specimens for analysis resulted in false reports of the Lyme disease spirochete in 51 patients.
Dr. Eva Sapi in her Research Laboratory at the University of New Haven possessed Living reference type 
Lyme spirochetes.    In Philadelphia, Penn, at the Advanced Laboratories where all blood cultures were done, 
the CDC contamination story was Impossible, because none of the alleged contaminants existed there.
The Only living borrelia in the Advanced Laboratories building were PRIMARY [New} { Unique}   
isolations of Borrelia from Human blood.  { A major step forward in the efforts to improve Testing for Lyme Disease}
The full power of the CDC is vast.  The CDC did not Fact Check the details before releasing their paper.
 Abuse of power is clearly in play here, to the detriment of public health.
[ 149 words]
------------------------------------------------------------------------------------------------------------------------------------------
 
 
 
 
Respectfully submitted, 
Alan B. MacDonald, MD, FCAP FASCP
8427 Benelli Court
Naples, Florida, 34114
email: [email protected]
august 26 ,2013
 
Disclosure statement: I am not an employee of any governmental agency 
or of any privateLaboratory. I am fully retired after 30 years of practice
 as a hospital diagnostic pathologist. I have never received any payments 
or other compensation of any kind from the parties in this dispute.
August 24, 2013
 
 
As of17:35 hrs on August 26 2013,  GenBank has not yet published or accepted
as valid, the CDC deposits KF170280 ,KF170281 , KF170282.
 
in GenBank, National Center for Biotechnology Information,
by  Advanced Laboratories.
 
Therefore, with no  GenBank validated  CDC deposits, there is no way to
draw any scientific conclusions about the   previously GenBank accepted
 Advanced laboratories DNA sequence  deposits from Human borrelia Culture
 positive DNA sequences.
 
The CDC  position  is scientifically Untenable, in this matter/
 
If I do not hear from you promptly, I shall escalate this matterto
the the US Senate Oversight Committee for the Centers for Disease Control
for  investigation  of the possibility of Scientific Misconduct.
 
No Investigation will be forthcoming if:
1 .Johnson et all RETRACT their manuscript from the J. Clinical  Microbiology
2. The Director of the CDC issues a Press Release, with a Public apology 
    for a CDC Flawed  Science  publication in an  international
     Academic Journal { Journal of Clinical Microbiology}
 
 
Yours truly
 
Alan B. MacDonald, MD Fellow of the College of American Pathologists
 
I am sending copies under separate cover  of this letter to:
 
Sen. Kristen Gillebrand,  New York
sen. Richard Blumenthal, Connecticut

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Originally posted by poppy:
Hey moderators, why is a troll being allowed to stay here? Please ban this guy. No patients should be exposed to continuing abusive behavior from someone who calls them cult like. Get rid of ihcTom!!!!!

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Posts: 786 | From UK | Registered: Oct 2007  |  IP: Logged | 

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Originally posted by lhctom:
One of two things has happened.

1. After being exposed and dealt a stunning blow by Dr Alan Macdonald, the Monster from NIH has turned tail and fled, like the coward he is.

or

2. Our good moderators, seeking to protect us from frauds and con merchants, have realised that he is back here for the nth time, pretending,yet again, to be a patient.

And though Ed still has a position at NIH, and still retains respectability as a member of various prestigious scientific societies like the American Association for the Advancement of Science (A.A.A.S.). our good moderators have understood what a hateful fraud he is, and slung him out right on his A.A.A.S..

Regardless of which has happened, I would like to make an URGENT request, not only of the moderators, but of every person here, whether you have participated in this thread, or merely read it:

Please SAVE any files you may have showing what he said on this forum.

Please SAVE them both in electronic form, and in HARD COPY.

Moderators, please RETAIN any technical, server-based or other data in any form that you may have, that might allow the origins of his messages to be traced.

Please everyone SAVE this material, and STORE it SAFELY. Because one day it could form part of a huge dossier of important evidence of the FRAUD perpetrated by US government scientists, and their colleagues in other countries such as mine, fraud that has resulted in the suffering of MILLIONS of people.

I know I joke a lot and use a lot of sarcasm. But I am deadly SERIOUS about this point. Things will never improve until all these morally-challenged Denialists are EXPOSED.

Elena Cook

Paranoia is a delusional disorder characterized by the dominance of one delusion or false belief without any other sign of mental illness. The person’s behavior depends on which delusion they have. For example, a person who has a delusion of persecution believes that other people are spying on them or plotting to harm them in some way. Online bullying and manipulation of others can be a result of a delusional paranoid disorder – for example, the person believes they are being stalked online as part of a conspiracy and accuse anyone with a different opinion as being the “stalker” and an agent of the conspiracy. Fears are described as paranoid when they are rigid and exaggerated and not based in fact. They are unable to consider opinions divergent from their own. It becomes a progressive personality disorder of delusions of persecution with extreme grandeur and is strenuously defended with convoluted logic and reason. It includes systematized delusions ascribing hostile intentions to others often driven by a sense of a mission. They are easily offended, readily assign harmful meanings to other people’s views and quickly become hostile, aggressive and argumentative. They often collect weaker personalities around them forming a dangerous cult that further drives their delusions.

best regards,

Your friend Ed

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Originally posted by lhctom:


"A horse is a horse, of course, of course,
And no one can talk to a horse, of course,
Unless, of course, the horse, of course,
Is the famous Mr. Ed!"

http://starkravingviking.blogspot.com/2006/08/lisa-masterson-of-uk-victim-of.html


http://www.actionlyme.org/SWEEG_STALKING.htm

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Originally posted by lhctom:



Big Eddy M

http://web.archive.org/web/20030625044352/http://qis.net/~edwardmc/ [/QB]

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