Marnie
Frequent Contributor (5K+ posts)
Member # 773
posted
I'm gonna get to the bottom line first..how's that for a change? ;-)
"Serine acetyltransferase is a key enzyme in the
sulfur assimilation
pathway of bacteria and plants, and is known to form a bienzyme complex with O-acetylserine sulfhydrylase, the last enzyme in the cysteine biosynthetic pathway."
Bb has replaced all Fe-S enzymes with those that use Mn. Bb does not use Fe or S.
If the gene for serine acetyltransferase (cysE) is NOT present = biofilm.
***Extracellular secretion of the Borrelia burgdorferi Oms28 porin*** and Bgp, a glycosaminoglycan binding protein
Proteomic liquid chromatography-tandem mass spectrometry analysis of tryptic fragments derived from strain B31 culture supernatants confirmed the identity of the 28-kDa species as Oms28 and revealed a 26-kDa protein as 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs-2), previously described as Bgp, a glycosaminoglycan-binding protein.
The ***release of*** Oms28 into the culture medium is more selective when the spirochetes are in logarithmic phase of growth compared to organisms obtained from stationary phase.
As determined by immunoblotting, stationary-phase spirochetes
released OspA, OspB, and flagellin.
Oms28 secreted by strains B31, HB19, and N40 was also recovered by radioimmunoprecipitation.
This is the first report of B. burgdorferi *** protein secretion*** into the extracellular environment.''
Above link only if you want to get REALLY into taking apart that protein.
``These exoproteins may be important in helping the spirochete to survive within the host, evade the immune system, and directly or indirectly cause pathology.
Several exoprotein genes have been cloned and recombinant protein will be used to test hypotheses that these factors play a role in the host-parasite interaction.
Oms28, a previously described pore-producing protein, may damage host cells.
Enolase, which is an enzyme involved in glucose metabolism and related to the same enzyme in streptococci that triggers protein degradation, may serve as a spreading factor following mammalian infection.''
Although SAT (serine acetyltransferase) of Escherichia coli, which catalyses the first step in cysteine synthesis."
Serine acetyltransferase is a key enzyme in the
***sulfur assimilation pathway***
of bacteria and plants,
and is known to form a bienzyme complex with O-acetylserine sulfhydrylase, the last enzyme in the cysteine biosynthetic pathway."
No need for sulfur = biofilm. No cysE gene(serine acetyltransferase)= biofilm.
Fe can destroy Bb...sulfur too?
Logically Bb does NOT want serine, esp. phosphatidylserine which rebalances the HPA axis.
Our most dangerous pathogens are those that use Mn.
Posts: 9424 | From Sunshine State | Registered: Mar 2001
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hiker53
Frequent Contributor (5K+ posts)
Member # 6046
posted
Layman's translation for your wonderful research, please.
-------------------- Hiker53
"God is light. In Him there is no darkness." 1John 1:5 Posts: 8879 | From Illinois | Registered: Aug 2004
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SForsgren
Frequent Contributor (1K+ posts)
Member # 7686
posted
You mentioned Mn - Manganese. Can you comment further on how these bugs use Manganese? Manganese is a key in assimilation of bone and avoidance of osteoporosis which seems to be a problem that many of us develop over time.
-------------------- Be well, Scott Posts: 4617 | From San Jose, CA | Registered: Jul 2005
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posted
From my understanding, it's Magnesium, calcium, boron, vit D, vit K and Phosphorus, needed to prevent osteoporosis. Bb likes Manganese, it seems to be what Marnie is saying.
Posts: 153 | From England | Registered: Jun 2008
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Keebler
Honored Contributor (25K+ posts)
Member # 12673
posted
-
Just tell me what I'm supposed to do. I just can't think anymore -
(wonder if Ingrid Bergman had a little lyme brain going on in "Casa Blanca" - " . . . I can't think anymore . . . ").
-
I have read that allicin will help dissolve Bb biofilm but allicin is far to speedy for me.
posted
Marnie says above, "" Our most dangerous Pathogens are those that use Manganese "" As Manganese seems to be in a many number of foods, then it could be hard to avoid. To my understanding, not good news. Posts: 153 | From England | Registered: Jun 2008
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