Lymedin2010
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***Cyst forming videos directly in our Lyme Diseased human blood:
***Atypical spiro to "Tennis Racket" form & then to Cyst (Discoid Gemma type) directly in HUMAN BLOOD. Tennis racket forms are widely known & seen in Borrelia obtained from ticks & wild grown in culture. https://www.youtube.com/watch?v=Eg_Id74a_4I
Nice video work. The baking soda brushing had caused motile spirochetes to disappear short term---but they came back after she discontinued the baking soda.
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It does seem as though biofilm plays a major role in harboring dental spirochetes-- once the laser treatment had eradicated the biofilm in her gums(alongside the teeth), her mouth was almost perfectly clear of dental spirochetes two weeks later.
Of course this is much more difficult for Lyme patients-- since spirochetes are inside our red blood cells.
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Lymedin2010
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Time Lapse 15s intervals for 14hrs of fresh smear & then sped up 10x. -400x w/Vaseline slip cover seal + cam zoom. Microwave (13s high), UV light (20 min) & heat exposed sample (20s) to speed up degradation.
Caution must be observed when trying to identify spirochetes in the blood, as other objects can easily be mistaken as spirochetes.
Red Blood Cells (RBC's) are biconcave in shape & when viewed on their side, they can be mistaken as small, stubby, string-like, & with bulbous tips. Some even appear as tennis rackets when stretched on one side.
Picture a coin standing on its side or a thicker rubber band folded onto itself. The rubber band will produce bulbous tips as the two opposite ends meet flatter in the center & naturally bulge out at the ends. Similar shapes can be seen when the bi-lipid layer of a rbc folds onto itself further to produce a narrow center & bulbed tips on it standing up.
These false spirochetes are: -Usually stubby & thicker than a real spirochete. -Usually very stiff & moves uniformly with no wave motion.
You can follow this video from scratch many times over & pick a new focal point to see what ultimately happens to the object in question. You might make a new discovery that I have not noticed with my bad Lyme eyes.
So now we have 3x objects in the blood that can be mistaken as spirochetes.
1) RBC's that are positioned on their ends standing up toward the observer (this video).
2) Footings & protrusions formed from moving White Blood Cells.
3) Fibrin strands that are stand-alone & that have not attached to other fibrin strands or blood components.
Now for the contrast of the ideal spirochete in my blood in this next video. -It has bulbous tips. -It moves, spirals, or produces waves with some aggression. -I see many of these to avoid mistaking any chance occurrences.
Lots of weird rod thingies in your mouth that don't look like oral or borrelia spirochetes. Did you scrape at the gumline to get the biofilm colonies at the gumline?
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posted
Oh sorry--- I see that you did scrape the sulcus (in the video info box.) I wonder what the rods are?
I really need to get a microscope soon-- I want to see if 2 grams a day of NEEM has any effect on my blood. I also want to to look at the effect of Manuka honey, plus foods with high saponin content. I'm just afraid Ill spend a lot and get a crappy scope since I dont know much about them..
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posted
This thread is very interesting. I have not read all the posts yet, just a few quick questions first: 1. Are those photos and claims on Lymephotos.com true or false? 2. How long does it take for the bacteria to change from one form to another? (time frame to rotate abx??)
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Lymedin2010
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I purposely slacked on brushing to be able to take that sample. Before I slacked off & brushed with baking soda, I could not find a single organism. I don't know what these are exactly, and when I asked an experienced dentist who does microscopy what these are, he just called them plaques with rods. There are so many oral flora we don't know about.
It takes seconds for them to go from spiro to cyst. It can take hours to go to SOP form & all depends the solution they are in.
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posted
The interesting thing about that video, though, is that I wouldn't consider those to be bacilli.
They look much too large for that. I mean their length in respect to width. What resolution was that?
I'd be interested to look at that under dark field.
I might take a swab myself to throw on the microscope. Might even though a sample or two in BSK. Just something for fun.
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As always, watch in HD 1080.
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TNT
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AWESOME JOB! That's the clearest video I've seen yet that shows the conversion to cystic form. A great example of how quick they can change.
dude, are you still using your Amscope? If you are, I'd say your captures are pretty good even with that.
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posted
Yes, I am still using my cheap Amscope T-490 Darkfield. I purchased the 100x oil dark field lens, which is what you are seeing there. No doubt that if had purchased a more expensive one, the image would be at least a little sharper.
Unfortunately, filming the change was affected by the aberration from the RBC's it was in between, so that little extra light coming off those cells blocked what would have otherwise been a perfect opportunity to film it.
You kind of miss the exact moment the change occurs because of this. I really wanted to see exactly HOW it went about the change, but that light obscured it.
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Lymedin2010
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Great video & I had missed that one. I will add it to the collection.
When you take the mouth sample, just scrape off some tartar between your gums & teeth. Take the sample from your back molars, as it is more moist there & then spread it back & forth on the center of the slide. Place the cover slip & then add Vaseline to the edges to avoid quick dehydration if you plan on looking at it at lengths.
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if I purchase a good used TRINOCULAR microscope, can I purchase an additional "Phase Contrast" lens later-- or does the scope itself have to be constructed initially for phase contrast use?
I just want the option of purchasing a phase contrast lens--- if Im finding that the regular compound with darkfield is not giving me good video quality.
It looks as though Phase Contrast provides the brightest and best images.
The Amscopes available on Amazon seem to be a pretty good deal for the money.
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Yes-- excellent, DudeKansas-- you can see the classic "loop" beginning to form in the middle of the spirochete..probably a precursor to the cyst-- although I have seen vids where the cyst forms very rapidly with no loop and you can see the spirochete inside its protective cyst, gyrating ---and even propelling the cyst.
The dangerous thing about cysts is that when they hatch later (under more favorable conditions) there can be up to 12 baby spirochetes inside.!!! This is why doxycycline is potentially harmful --- doxy promotes the conversion of live spirochetes into cysts, as per Sapi's research. A person should probably always be taking Flagyl (cyst killer) while they are on doxycycline or Amox-- as per Burrascano's protocol. It takes cysts 18 months to die (become unviable) on their own, as per Brorsons' research.
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quote:Originally posted by WakeUp: Question for Lymedin2010--
if I purchase a good used TRINOCULAR microscope, can I purchase an additional "Phase Contrast" lens later-- or does the scope itself have to be constructed initially for phase contrast use?
I just want the option of purchasing a phase contrast lens--- if Im finding that the regular compound with darkfield is not giving me good video quality.
It looks as though Phase Contrast provides the brightest and best images.
The Amscopes available on Amazon seem to be a pretty good deal for the money.
Look up the microscope and see if there are any additional components you can add. For some microscopes, you can buy kits to convert them to phase contrast.
Make sure the microscope you are looking at is convertible. Go to the manufacturers site and look up the specs for the specific model.
It is possible, just not for every microscope.
But yes, you can buy it and convert it to a phase contrast microscope if there is a kit available.
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I'll have to look and see if I still have some old photos.
I recorded video of fibrin strands that were visible on the slide with the intention of using it to show what they look like.
They are easily differentiated from a spirochete if you know what you are looking at.
I'll see if I still have that footage saved somewhere.
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Lymedin2010
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Phase depends on scope & just see if phase exists with the particular model in question. The condensor & objective lenses are always tied to the phase viewing for maximum visuals. Many scopes can be converted though.
This is my other video showing fibrin clearly with the same setup & only difference being LED vs CFS bulb replacement. A big difference in how the camera system interprets the lighting & what is revealed.
Lymedin2010
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FYI, I use my Reichert over my Zeiss now only for one reason. The fact that the lighting system uses mirrors that reflect light from the back of the scope. In this way I am able to use a LED light, which produces even bigger unfavorable visuals, and the end result is less heat & ability to do time lapse without any major focus creep issues. The condenser on the Reichert is an Abbie & it is really inferior and tends to produce slight blurry/distorted visuals.
My Zeiss is VERY sharp because of condenser & Zeiss optics, but I forego the sharpness for the time lapse benefits. Another benefit is that I can run the LED light in the back of my Reichert with very little light spilling into the room & disturbing all night time lapse.
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I also use a high lumen LED for my microscope. I bought an LED headlamp for about 30 dollars, I can't remember the what the lumen rating was. Maybe 600? But there is a stark difference between what is visible with that light, and what is visible with a comparable level of illumination from a standard CFL bulb or any other standard bulb.
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Lymedin2010
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Yes, most definitely by the human eye. However the camera has a very difficult time picking up LED light, at least the crappy USB interpretation software does.
CFL interprets much more natural & better with my Amscope USB cam. You can see the difference of the 2 light source in the video I last posted, even with my bad combo of glass on the Reichert there is a huge differnce in detail.
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posted
Yea, I might attempt a higher lumen bulb in the near future.
My headlamp goes through AAA batteries like the cookie monster does with cookies. Batteries are expensive.
But I did notice, with darkfield, that the LED light, at the same lumens as a regular bulb, provided much better results both to the eye and with my DSLR.
Which bulb do you use currently?
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TNT
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quote:Originally posted by Lymedin2010: FYI, I use my Reichert over my Zeiss now only for one reason. The fact that the lighting system uses mirrors that reflect light from the back of the scope. In this way I am able to use a LED light, which produces even bigger unfavorable visuals, and the end result is less heat & ability to do time lapse without any major focus creep issues. The condenser on the Reichert is an Abbie & it is really inferior and tends to produce slight blurry/distorted visuals.
My Zeiss is VERY sharp because of condenser & Zeiss optics, but I forego the sharpness for the time lapse benefits. Another benefit is that I can run the LED light in the back of my Reichert with very little light spilling into the room & disturbing all night time lapse.
Shhhh.... you're making me jealous for a research grade scope. Posts: 1308 | From Eastern USA | Registered: Oct 2013
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Lymedin2010
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This bulb produces perfect light & interpretation by my USB camera. I show the bulb at time 3:20. https://youtu.be/7FlpCs0MiS8?t=201
Don't forget if you buy a used microscope expect to sell it back when you want at the same or similar price. So really you are renting it for months on end & can sell it if you are pressed for monies.
PS I am looking forward to see what you guys are finding in your mouths. I checked my sperm & nose in the past & nothing significant. Next time I will be checking a wound or a sample from thin skin areas that are red & inflammed.
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posted
Which microscope are you using? What about the one from Michael Coyle?
When you put a drop of blood on the slide do you then take a needle and crush the cells to make the bugs jump out. Then put a cover slip on the blood and view in microscope?
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Lymedin2010
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You can also gently press down on the slide afterward & before putting on oil. Do this to make the field of view even narrower & ultimately sharper.
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posted
Hey, Lymedin, I remember watching that video when I first picked up some bulbs to try out. However, I went with what I could find at Wal-Mart and Home Depot.
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Lymedin2010
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There are many that work, but the important thing to look for is DAYLIGHT white light (5000K), as it comes out clearest & best in recordings.
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posted
Hi Guys--- Well I finally bought the Amscope Trinocular, and my very first view of my red blood cells looked almost exactly like this ( one hour ago) (I don't have the a camera set up yet) Except that the black spots were a tad bigger in size, and my red blood cells looked more irregularly shaped and clumped than these ones:
I guess I am really sick based on an initial view of my blood.. I did not want to admit it.... but pictures do not lie.
Sadly, the 100 objective is not that great for my Amscope (its a bit dark and a little bit fuzzy)--- I was counting on this--- but the 40 objective is super clear/bright and and I could clearly see the black spots in about 65-70 percent of my red blood cells. I guess these scopes are all about the lens.
I scraped a small morgellons lesion and looked at the blood under darkfield--- it was absolutely beautiful!! It looked like an amazing fractal.
Well I'm guessing Bartonella may be my biggest problem-- most of my red blood cells are massively infected. The black spots are quite large. There were also some kind of balloons inside many of my cells Ill post pics when I get the camera--- but I may return this Amscope trinocular or purchase a different 100 lens for it-- since this 100 lens is not that great. Any suggestions on an excellent brand of 100 objective lens?
Thanks for all your posts regarding microscopes---At least I finally made the plunge..!!!!
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posted
WakeUp, which Amscope did you get? I use an Amscope. Once you get used to using it and figuring things out, your skills will greatly improve and you might be impressed what you are able to get out of it.
Also, if you don't want to put any money into a better 100x lens, you can get eye pieces that will increase the magnification.
The standard is usually 10x, but you can by 15 and 20 also. So you can get a resolution close to 1000x without spending nearly the same amount of money. Just a thought.
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Lymedin2010
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Which model did you buy exactly?
That image above was stained, did you stain your blood sample? It may be artifacts.
"Fig 1. Write –Stained Bartonella bacilliformis in blood of an Oroyo fever infected human (Source: The Prokaryotes)"
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posted
Kansas---Thanx for the encouragement--- I was disappointed with the 100 lens but perhaps its my lack of experience. This is the Amscope I got: (I know I overpaid, but I just had to take the plunge and start-- )
Lymed2010-- Well Im very new at this, but I'm almost positive those black spots inside the red blood cells were not artifacts....... but I will the buy the stains and keep looking.
But ---- my blood looks like total crap!!!! No wonder Im in bed most of the day.
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TNT
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quote:Originally posted by WakeUp: Hi Guys--- Well I finally bought the Amscope Trinocular, and my very first view of my red blood cells looked almost exactly like this ( one hour ago) (I don't have the a camera set up yet) Except that the black spots were a tad bigger in size, and my red blood cells looked more irregularly shaped and clumped
Way to go for taking the plunge! Don't be too discouraged, this tool will help give you some direction once you get accustomed to what you are looking at. The giemsa staining is EXTREMELY helpful I have found.
If you were only looking at live blood, it is very possible that those RBCs were merely crenated, not infected. I have found live blood great for seeing the ketes, but staining much better for differentiating objects. If you get a bunch of purple dots on your RBCs on a giemsa stain, then you may have cause for alarm.
Welcome to the gang!!
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I have added a 100x Oil Immersion lens for Darkfield at 1000x, which also required a new condenser.
However, pick up a set of 20x eyepieces if you want a greater magnification without the extra cost. I was pleasantly surprised with the resolution from the 20x eyepieces, with a dry dark field condenser.
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posted
Perhaps I'll make a video demonstrating my setup, and set it to private (might have my face in it)? For y'all to view.
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bluelyme
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Way to go wakeup...dude that would be most insightful..you all are on the front lines! on the staining can the chemicals be purchased w/o commercial liscence ?...is a fixer necessary?
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Yes, you can purchase a majority of the stains anywhere, and some are relatively cheap.
Look up staining kits on Amazon. Gram Stain kits, Giemsa/Wright, etc.
Methanol can be purchased if you use that as a fixative, etc.
You start running into trouble finding stains when you get into the more specialized stuff.
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posted
Yes, you can purchase a majority of the stains anywhere, and some are relatively cheap.
Look up staining kits on Amazon. Gram Stain kits, Giemsa/Wright, etc.
Methanol can be purchased if you use that as a fixative, etc.
You start running into trouble finding stains when you get into the more specialized stuff.
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Lymedin2010
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quote:Originally posted by thatdudefromkansas: Also, upgrade the light for dark field. The bulb was not nearly bright enough.
Take Lymedin's advice on bulb, and get a cheap 10 dollar lamp from wal-mart or something to use.
I use a 30 dollar LED headlamp, i think 1600 Lumens, but adjustable.
True--- the problem with the 100 objective is the lack of enough light.. One of the reasons why I hesitated to purchase the Amscope was the fact that I had read reviews saying that scopes with LEDs (not halogen) lights are much better and brighter-- and this Amscope does not have a led light. I will try to supplement my light with a bright lamp first-- and then perhaps buy the headlamp.
Ill also buy the stains--- but I am quite positive that I saw the dark spots inside my red blood cells -----without a stain!!
I had fiddled with the fine focus---- and then the black spots inside the cells suddenly came into view-- it was stunning-- these spots were not outside of the cells.
This also explains why I have had burning on my feet for the last 6 months-- a classic sign of bartonella. My immunity has also been poor in the last few years--- and Bartonella is an immune suppressant. I have been bitten by at least 3 ticks over the past 25 years, and have had 2 bullseye rashes, and one smaller bright red rash from these bites...
A few years ago I successfully diagnosed Cedar Rust on my Gala apple trees---- by just using 1 sample diseased leaf--- and then comparing it to dozens of online photographs of different apple tree diseases--- and BINGO --- it was cedar rust (rust colored spots on the leaves)!
I had several Cedar trees growing--- right next to my apples- and I had no idea that the cedar trees were infecting my apple trees with spores... Pictures are worth a thousand words.. Anyway, I planted Liberty apples were are resistant to cedar rust. (The Gala apple trees all died!!)
Its very gratifying to find the source of a problem---- and not to have to shell out thousands of dollars from so-called "tree experts" and "doctors" ---- who often provide a wrong diagnosis----- after taking lots of your money.
I am researching Bartonella herbs/treatments now--- but will see if I can get pics of the dark spots inside the cells--with and without staining. I might even consider trying to find out which strain of Bartonella it is with a decent blood test, once I have replicated the results several more times..
The camera is coming soon.
Have a great weekend.
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Wow--- nice resolution and video. I like the cute white blood cell.
The red blood cells at the beginning of the video do not look normal and healthy though!! Normal and health red blood cells should be round, of the same size and not configured in clump like chains--- as is the case in your video.
The cells are showing rouleau (sludge blood) which indicates undigested sticky protein-- meaning the red cells are not charged negatively (repelling each other properly) , and are thus not repelling each other as they should--- and instead they are clumping in the long chains visible at the beginning of the video-- which probably means you have low energy. The red cells do look pretty healthy though, even though they are in chains-- their shape is nice and plump with smooth edges-- far better than how my cells look. (My edges are smooth, but the shape of the cells is not round/plump-- instead a lot of my cells have a smooth irregular blob shape with black specks inside.)
I saw a video of live blood of before and after drinking barley grass juice-- and the positive effect on the blood rouleau was amazing in just one hour. This effect can also be achieved with pycnogenol, and supposedly with mangosteen (or was it noni?) juice. Exercise also causes red blood cells to separate from their chains and to declump. Pancreatic enzymes also help with the digestion of proteins-- so the stickiness of the red cells is lessened and chains fall apart.
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Another thing I noticed in your most recent blood video was that many of your red cells have a weird paisley or carrot root type shape--- when they should be nice and round and plump. This paisley shape is not normal, I think.
I think Dr. Chambers in the video above believes that weird paisley shape is due to a parasite. (at about minute 6)
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Lymedin2010
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Only the first 2:30 is the untreated blood (pre-heat). This was a wetter mount than usual, as I thought the heat would lyse most of the rbc's.
When you see rbc's standing on their sides, it means the slip cover was not compressed as much & there is space between slide & cover for clumping. If I create a thinner smear & compress the slip cover, then it does not look as bad.
Also, if one takes venous blood & not finger prick blood, then it will look healthier as you don't have to squeeze the finger & cause some trauma.
All depending on what you ate, drank, & meds you took before extraction, blood can look different. The slight changes in preparation from slide to slide can also make it look different too.
TNT
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quote:Originally posted by Lymedin2010: This was a wetter mount than usual,...
When you see rbc's standing on their sides, it means the slip cover was not compressed as much & there is space between slide & cover for clumping. If I create a thinner smear & compress the slip cover, then it does not look as bad.
Also, if one takes venous blood & not finger prick blood, then it will look healthier as you don't have to squeeze the finger & cause some trauma.
All depending on what you ate, drank, & meds you took before extraction, blood can look different. The slight changes in preparation from slide to slide can also make it look different too.
I agree. It's interesting the conditions some people try to "diagnose" by just the appearance of the RBCs on a particular sample. What I have found after looking at perhaps hundreds of samples is that the amount of rouleaux and crenation depends almost entirely on the volume of blood under the coverslip.
Try this experiment for comparison: Place two drops of blood on a slide, one on each end with enough margin on the ends that your coverslips will not extend over the ends of the slide.
On the one side, use a very small drop of blood; on the other, use a large drop of blood. The large drop will easily spread across the area of the whole coverslip, whereas the small drop won't even reach the edges. Then look at both samples.
In the large drop, the RBCs will have much rouleaux and crenation, but on the small drop, they will appear round, evenly spread, and healthy.
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Lymedin2010
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Yes, I have seen similar results TNT.
-1 drop 37% HCL on 1 drop finger pricked blood. -400x w/cam zoom.
RBC's & WBC's have lysed. String-like objects observed, but no definitive signs of spirochetes.
Although, my subsequent tests with diluted vinegar produced tons & tons of spirochetes & tons of SOP with subsequent bleb release into the blood plasma. Video to come eventually.
************************************************
Freezing blood for 24 hrs did not produce any spirochetes that emerged out of cysts either as I suspected might happen after long term observations.
posted
Ooops-- it was Goji juice--- (not mangosteeen juice) that rectifies the blood Rouleaux "stickiness" problem (sludge blood) seen in live blood, within 24-48 hours-- according to this live blood video presenter, who is supposedly a Johns Hopkins trained medical doctor (hopefully not paid to sell Goji):
The narrator basically says that blood rouleaux is also related to blood acidity. Another way to alkalize is to take 1/4 teaspoon of baking soda in a quart of water or just drink trader joe's alkaline water.
So far I'm not seeing much Rouleaux in my own blood--- nor is there any crenation (scalloped edges)-- but my RBCs are oddly deformed, and I am still seeing little black dots clinging to the outside of the red blood cells-- although not as many as yesterday. (I saw 15 cells with black dots attached per screen today, plus I think I might have seen two spirochetes with bulbous ends.)
One of those live blood experts was saying that live cells with scalloped edges usually indicate some sort of toxin in the body and/or oxidation. Goji has one of the highest antioxidant levels (Orac score--Oxygen Radical Absorbance Capacity) of all juices.
Pycnogenol also has a very high Orac score. It would be interesting to see if Goji or Pycnogenol supplementation for a few days can reduce the scalloped edges and rouleaux seen in live cells.
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