__________________________________________ At 7:00 Dr. Willy Burgdorfer also mentions that he found filarial worms in the ticks as well. This is confirmation of what Dr. Alan MacDonald has found in 10 out of 10 MS patients...Filarial Nematodes with Borrelia living within the worms as endosymbionts.
__________________________________________ At time 40:24 Willy Burgdorfer (the guy who discovered Lyme Disease) also confirms that the Elisa & Western Blot test (the antibody tests) that test for spirochetes, only test for ONE strain of the spirochete. It test for the Borrelia burgdorferi strain B31 & no mutation from that strain & none of the other new Borrelias to have been discovered thereafter to cause LD. At that time there were 39 known Borrelia species & now there are well ~200 species in the US alone.
__________________________________________ This confirms the ring of echoes from many other LLMD's & sources, including what Dr. Alan MacDonald says about the poor testing in this video at 17:21. They only test for ONE of the strains of Borrelia (Bb 31) & therefore many people who have Lyme Disease fall through the crack & are diagnosed with all the various neuro diseases which encompass the same exact symptoms as Lyme Disease + the particular damage it causes to that label (Fibromyalgia, ME/CFS, MS, Parkinson's, ALS, IIH...etc). Perhaps not all of the labeled diseases are caused by spirochetes, but one way in which they can develop.
bluelyme
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Roceph aint doing much intercellualarly.. on a bright note i made a darkfield condenser out of polarizing films and got a led upgrade for my ancient p.o.s..
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TNT
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That's some great work, blue!!! That's some really nice darkfield with makeshift apparatus.
How do you know Rocephin isn't doing doing much intracellularly? Technically I know it doesn't get intracellular, but I don't see ANY ketes in your video. So, it must be doing something. For certain it's either killing them, or forcing them out of the plasma into cells and tissues. Zithromax is intracellular.
What kind of scope did you get then?
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So, has anyone in this thread seen successful eradication or a decreased load in any single Borrelia form in serum with a specific kind or combo of antibiotics and/or herbs??
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TNT
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quote:Originally posted by birthdaysuit: So, has anyone in this thread seen successful eradication or a decreased load in any single Borrelia form in serum with a specific kind or combo of antibiotics and/or herbs??
I've been able to see a significant lowering of my spirochete load with BVT (and ABX).
I also was able to see a lowering of possible fungal loads with different modalities such as SF722, Nystatin, and diet. The fungal forms are a little speculative, since I was not able to positively identify what I assumed were fungal forms. But whatever they were are pretty much completely gone. Earlier lab tests did show that I had a high load of candida, but I have not retested.
My last stained smear showed no Anaplasma morula, and the one before that (almost a month ago) showed a waning infection, as I only saw faint traces of the morulas on that slide. This was during/following a few months of Tetracycline and Omnicef. Interestingly, I do not remember seeing any morulas prior to being on Levaquin and Tetracycline together. So, Anaplasma treatment has taken some time. But, with continued treatment with Tetracycline (mainly), evidence of infection has completely gone away and now my response to the Tetracycline (and Omnicef) has signaled it's time to transition and address other infections. That's where I'm at now, in transition.
So, yes, you can definitely see a visible change with treatment and monitor treatment success via microscopy.
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quote:Originally posted by birthdaysuit: So, has anyone in this thread seen successful eradication or a decreased load in any single Borrelia form in serum with a specific kind or combo of antibiotics and/or herbs??
I've been able to see a significant lowering of my spirochete load with BVT (and ABX).
I also was able to see a lowering of possible fungal loads with different modalities such as SF722, Nystatin, and diet. The fungal forms are a little speculative, since I was not able to positively identify what I assumed were fungal forms. But whatever they were are pretty much completely gone. Earlier lab tests did show that I had a high load of candida, but I have not retested.
My last stained smear showed no Anaplasma morula, and the one before that (almost a month ago) showed a waning infection, as I only saw faint traces of the morulas on that slide. This was during/following a few months of Tetracycline and Omnicef. Interestingly, I do not remember seeing any morulas prior to being on Levaquin and Tetracycline together. So, Anaplasma treatment has taken some time. But, with continued treatment with Tetracycline (mainly), evidence of infection has completely gone away and now my response to the Tetracycline (and Omnicef) has signaled it's time to transition and address other infections. That's where I'm at now, in transition.
So, yes, you can definitely see a visible change with treatment and monitor treatment success via microscopy.
Whats BVT? And Lyme and many other infections are in fact tissue infections. They like soft tissue, marrow, cartilage and joint fluid. Just because you do not see any in serum does not mean you have eradicated the infection. A biopsy would help to help distinguish this.
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TNT
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quote:Originally posted by birthdaysuit:
quote:Originally posted by TNT:
quote:Originally posted by birthdaysuit: So, has anyone in this thread seen successful eradication or a decreased load in any single Borrelia form in serum with a specific kind or combo of antibiotics and/or herbs??
I've been able to see a significant lowering of my spirochete load with BVT (and ABX).
I also was able to see a lowering of possible fungal loads with different modalities such as SF722, Nystatin, and diet. The fungal forms are a little speculative, since I was not able to positively identify what I assumed were fungal forms. But whatever they were are pretty much completely gone. Earlier lab tests did show that I had a high load of candida, but I have not retested.
My last stained smear showed no Anaplasma morula, and the one before that (almost a month ago) showed a waning infection, as I only saw faint traces of the morulas on that slide. This was during/following a few months of Tetracycline and Omnicef. Interestingly, I do not remember seeing any morulas prior to being on Levaquin and Tetracycline together. So, Anaplasma treatment has taken some time. But, with continued treatment with Tetracycline (mainly), evidence of infection has completely gone away and now my response to the Tetracycline (and Omnicef) has signaled it's time to transition and address other infections. That's where I'm at now, in transition.
So, yes, you can definitely see a visible change with treatment and monitor treatment success via microscopy.
Whats BVT? And Lyme and many other infections are in fact tissue infections. They like soft tissue, marrow, cartilage and joint fluid. Just because you do not see any in serum does not mean you have eradicated the infection. A biopsy would help to help distinguish this.
I'm not sure why you would post a question about pathogen loads on the microscopy thread if you want more validation than microscopy. The simple fact is Bb is a blood infection, and loads in the bloodstream would generally correlate with loads elsewhere. I agree that just because one does not see them in the blood does not guarantee that there is no infection present. But, viewing pathogen load in the blood over time is a good indication of which way the infection is going.
A biopsy would be extremely inefficient at gathering data. That is why serology is almost exclusively the testing used. PCR is the gold standard currently used in the mainstream medical community regarding verification of actual infection, but that is inherently flawed for detecting most low-grade chronic infections. Just because PCR is negative does not negate the good possibility there is infection present.... not just for Bb, but for all the infections. PCR is like fishing. Just because you don't catch any fish does not mean there are no fish present. It just means you did not catch any with that cast or net.
You did ask if we are able to see decreasing loads, and we definitely are able to see that via our own microscopy.
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TNT
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BVT is Bee Venom Therapy.
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TNT
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Here are some pictures I've been wanting to post for a while now. They are pics from sterile swabs that I have stained.
These first 6 pics show a tongue swab from my own tongue of epithelial cells, candida, possible pseudo-hyphae, and bacteria.
#1. Candida on an epithelial cell:
#2. A colony of candida beside a colony of bacteria:
#3. Heavy load of candida on an epithelial cell:
#4. A small colony of candida beside a well-defined epithelial cell:
#5. Possible pseudo-hyphae:
#6. Another epithelial cell among some candida and possible pseudo-hyphae at the top of the pic:
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TNT
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Now to really gross you out!
Stained bacteria from pimple pus:
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TNT
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And, a sterile vaginal swab showing epithelial cells and bacillus bacteria:
1.
2.
3. A couple vaginal epithelial cells, chain bacillus bacteria, and a few possible WBCs:
4. A couple vaginal epithelial cells, bacillus bacteria, and very possibly, a lymphocyte:
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TNT
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And, a pic of another interest of mine....telescopy. But, only when I'm feeling good enough to get it out, which is not very often. Microscopy is SO much more interesting and insightful, but it's usually all "business." Telescopy is more for fun.
This pic taken through the ocular of my scope:
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I am searching for an LLMD that does live bloood darkfield microscopy analysis in Washington state.
Does anyone happen to know one ?
Thank you very much,
Julien
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bluelyme
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Its a ol unversity wesco i have Frankensteined into working for me ..still need to see about slr mount or other capture ... thanks for the full moon tnt and the swabs ,you are theeee bomb
welcome jules ...i dont think many docs do this ..mexico and sierra and dr j in md use microscopy . here is a list of darkefield practioners there are 2 in your state ...for a lot of research a little investment you can have a darkfield of your own ?..here is that list lymed gave
Lymedin2010
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TNT, those are awesome stains & well defined. I would expect to see more pseudo-hyphae though. Can you see them well defined without the stain? If so, you might want to do some time lapse...it would be awesome to see them develop.
I betcha the some of the commensal bacteria hold the key to Borrelia eradication. They must compete for the same resources in the wild & one may have already be secreting a protein, enzyme, or abx that eliminates spiros. I wish they would put all them one-by-one with Borrelia in vitro to see what happens.
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TNT
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quote:Originally posted by Lymedin2010: ThatDuke, did he remove all his videos...gone?
Sure looks like dude removed his whole channel. I can't imagine why he would have done a thing like that. Can someone get in contact with him and find out why?
Yeah, that's a nice scope, Lymedin. I just wish that more of these high-end scopes would be a complete unit. So many of them are missing the high magnification objectives. That really adds to the price. I saw a very nice Optiphot 2 with vertical fluorescence, plan phase objectives (including the 100x objective), and a 60x Plan Apo objective, go for $1650 just a couple days ago. A very sweet machine. Had everything you could need. The only thing you could have added-and probably should have- would have been a 100x iris objective. But the Plan Apo 60x would have done great darkfield too.
I think I actually posted the link to it a week or two ago.
It wasn't epi-fluorescence though. I guess I'm not sure the difference.
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TNT
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quote:Originally posted by Lymedin2010: TNT, those are awesome stains & well defined. I would expect to see more pseudo-hyphae though. Can you see them well defined without the stain? If so, you might want to do some time lapse...it would be awesome to see them develop.
I betcha the some of the commensal bacteria hold the key to Borrelia eradication. They must compete for the same resources in the wild & one may have already be secreting a protein, enzyme, or abx that eliminates spiros. I wish they would put all them one-by-one with Borrelia in vitro to see what happens.
I didn't do a wet mount of the tongue swab. Only the vaginal swab. Pretty neat on that one. Saw the same things, except in "black & white."
I might try a wet mount of a tongue swab sometime, now that you mention it.
I think there's a member on HW that has mixed saliva and blood. Says there's quite a show.
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TNT
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quote:Originally posted by bluelyme: Its a ol unversity wesco i have Frankensteined into working for me ..still need to see about slr mount or other capture ... thanks for the full moon tnt and the swabs ,you are theeee bomb
welcome jules ...i dont think many docs do this ..mexico and sierra and dr j in md use microscopy . here is a list of darkefield practioners there are 2 in your state ...for a lot of research a little investment you can have a darkfield of your own ?..here is that list lymed gave
Very good blue. I'm sure that ol' Wesco will do just fine. Especially with a handyman such as yourself.
Dr. M-the Bart guru-uses microscopy in his office too. Not live blood, just stained slides as far as I know. His focus is on Bart and protozoans. He has a SWEE-E-E-E-T scope. It's a high-end computerized research Nikon.
Blue, do you know why Dude took down his 'tube channel?
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Hey guys, still here. Had an issue with my email account and ebay and amazon hacked. Had to change everything and bring all accounts down unt I resolved it.
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However, if compare the image of N with the video I just posted.
It's a similar parasite-like propagation. It's possible that the intracellular infection, and reproduction, is supported in comparing what we see with how similar parasites go through their lifecycle.
If borrelia spp. is indeed intracellular in the course of its lifecycle: evasion of immune system and dissemination through the blood, but aided by the fact that it can harbor itself INSIDE erythrocytes for reproduction and immune system evasion, perhaps that is what we see in images and videos like what I posted.
The the bacteria acts like a parasite similar to malaria. Malaria is found in the blood.....but it also relies on the ability to harbor itself inside of red blood cells.
Just a thought as you carry forward.
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Use it, but compare with other sources. However, there are some good images in there to make comparisons with, and to use as a jumping off point for researching in other sources.
This source........I'm not too sure about it. Like I said, good for images etc. However, if does seem to be too live-blood-analysis cultish.
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Lymedin2010
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Your videos are back, but all the blood videos are gone.
Maybe the blood videos need to reset to public again, as I think you had that trouble before?
************************************* 2) Here at about 1:05 in Under Our Skin they show us a nice Borrelia burgdorferi (one of the Lyme Disease spirochete) that is atypical, which means it does not look like the typical textbook hard spirals but is much softer in spiraling movement.
************************************* 4) A major microscope manufacturer, CytoViva, put out this spirochete video. When I asked them how this was captured, they said that a university cultured Borrelia in the lab & added it to blood.
TNT
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That's a neat AND gross website, mustardseed2. I had come across it a while back and had forgotten about it.
Lymedin, I watched the video by Holly Ahern you posted and came across another of her videos that uses a published study to discount the Doxy 2-pill prophylaxis myth.
Looking back at old videos, it is crazy to see the difference from when I first got sick and how my samples look now after all the antibiotics.
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Also, those stains were tested on wet mounts.
So no need to dry, etc.
Apply stain to sample, then view. I might try it this weekend when I have time.
Just got to make sure the food coloring is free of particulates, etc.
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Lymedin2010
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I've encountered those blog case reports in the past too...good stuff & I have not visited the site for a while. There is lot to learn with Lyme related microscopy alone & one can spend a lifetime with pathogen microscopy & not get the complete picture.
I love the video from Holly Ahern, & DAMN she is to the point & in your face! No, Doxy does not cure!
I had tried the easter egg stains & various other stains in the past, but too many artifacts. I then bought some 1 micron filters to filter them, but never proceeded any further. Great idea though & I would love to see them in the live...you might pioneer a new method for us...waiting for your success.
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Lymedin2010
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The freezing method is really good, to just leave behind borrelia in cyst & hopefully it then wakes up for you..I am surprised no one has tried this yet? If I had more energy, I would have done more....going to soon hopefully. I may also try freeze & then stain with Wright/Giemsa.
We should all brain storm, as there must be a nice way to burst open all or most of the rbc's & just see babs & barotnella stream out. I am thinking various wavelength light, laser pointer, ultra sound....etc. Never got to this either, but I did try heating, microwaving, & then freezing to see what else I could learn. There is some nice results from freezing too, as I see many nice structures that could be bart or babs, just hard to prove as maybe it might be the wbc's organelles (lysosomes). So I am still waiting for more proof.
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Lymedin2010
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Holy crap Duke, you had so many spiros & they all look like true spirochetes. Do you have video of your most recent blood, to see the difference? One doctor who does microscopy says that she sees waxing & waning of spiros in the blood over time in hers & her patients too.
Lymedin2010
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Credit: MICHAEL ABBEY/SCIENCE PHOTO LIBRARY
Caption: Borrelia burgdorferi, the bacteria that causes Lyme disease.
______________________________________ Credit: MICHAEL ABBEY/SCIENCE PHOTO LIBRARY
Caption: Borrelia burgdorferi, the bacteria that causes Lyme disease.
______________________________________ Credit: MICHAEL ABBEY/SCIENCE PHOTO LIBRARY
Caption: Lyme disease bacteria. Light micrograph, using phase contrast, of a spiral-shaped Borrelia burgdorferi bacterium (at centre), the cause of Lyme disease in humans.
The third is a great image of a typical spirochete.
The others....you can see the slight variation in wavelength in its morphology.
I need to find some light filters to utilize.
Anyone else amazed that this seems to be the only place on the internet where we are doing this?
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Lymedin2010
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This is not the only place, there were 2 groups that opened & closed, because it fizzled out & there are a few groups on FaceBook. I gave the link in a previous post, but there are a few of them now. People from all over the world know about this & have seen the spirochete in their Lyme blood.
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That other one might have died out before I came across it.
Hopefully the other ones remain active. I see many people reference our board quite a bit. But even Lymeneteurope doesn't have a group like this.
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Lymedin2010
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What happens is the groups start & then a bunch of people are invited. Then everyone starts to post & there is a lot of interest & activity. After some time everybody learns & check their own and many see the spirochetes. Then they get bored, unless they find some other interesting pathogens. So over time there is less & less posts, because those that have been there the longest have seen & know about it & get bored...human nature. The juicier posts are from the older posts, when the group first started & many of these groups were started late 2015 or early 2016, there was a gold rush of Lyme microscopy realization then.
A list of FaceBook Groups below, I am in contact with most of the people there & they come from all over the world:
You seem to be quite involved in that group.
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TNT
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quote:Originally posted by thatdudefromkansas: I'll have to get involved there.
You seem to be quite involved in that group.
HEY!!! Don't forget about us here!
I won't do facebook, so Lymenet is my main group. I don't understand why people want their identity to be known to the whole world. Especially when it comes to something this controversial.
I see Peter Kemp is on that facebook group. Is S13 on it, too? I've wondered how he is doing. I don't see him post much at all anymore, and I hope he is doing well. He had the potential to really contribute to our staining learning curves. In fact, he did help me a lot.
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I'll be posting all videos and stuff here. =)
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TNT
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quote:Originally posted by thatdudefromkansas: yea, this is my main thing here.
I'll be posting all videos and stuff here. =)
Great!!!
-----------------------------
I found some distinct morulas in a friend's blood who is fairly healthy. He wanted me to look at his blood because he has been having some minor issues... nothing real serious. Some headaches and minor pain/discomfort in his liver/gallbladder area. Otherwise he is healthy.
So, I was completely taken back when I came across what appears to be Ehrlichia or Anaplasma. Interestingly, I found some morulas in some lymphocytes. I haven't seen that before.
I am almost certain he does not have Bb as he has a healthy number of NK cells. This is probably why he only has minor issues and is not chronically sick.
Again, I am more and more convinced that we are chronically sick because we are multiply-infected.
I came across some info about Ehrlichia commonly being sub-clinical in dogs. So, I am wondering if this is possible in humans as well. Perhaps, since we know it's definitely possible with Babesia. And, perhaps a sub-clinical infection with either Babesia or Anaplasma/Ehrlichia would set a person up for chronic Borreliosis once they are exposed to that.
Here is the article that mentions sub-clinical Anaplasma infections in dogs:
Notice that it says that morulas can occasionally be seen in lymphocytes.
[ 02-15-2017, 04:32 PM: Message edited by: TNT ]
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Lymedin2010
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You guys are upper echelon here, and there are many newbies there who need some direction.
I always post the most interesting stuff here, when I find it & try to collect new technology over there, as there are tons of it really. Also the newbies will see everything as pathogenic if they do not have some direction & even then they might be resistant & not believe at times.
Peter is there, but s13 has not identified himself. I may send him an email. There are a lot more lurkers there than posters.
Borrelia, babs, & bart can be with infection but asymptomatic & I so no reason why other organisms can present this way. Especially TBD's.
[ 02-16-2017, 10:39 AM: Message edited by: Lymedin2010 ]
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Lymedin2010
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THE SPIROCHETES IN OUR LYME BLOOD MEGA LINK:
************************************* ************************************* ************************************* I put together the best professionally documented videos of atypical forms of Borrelia burgdorferi, just as they look in our blood.
1) Holly Ahern, the Assistant Professor Microbiology at SUNY Adirondack does an interview here & they show us Borrelia spirochetes in the blood.
************************************* 2) Here at about 1:05 in Under Our Skin they show us a nice Borrelia burgdorferi (one of the Lyme Disease spirochete) that is atypical, which means it does not look like the typical textbook hard spirals but is much softer in spiraling movement.
************************************* 4) A major microscope manufacturer, CytoViva, put out this spirochete video. When I asked them how this was captured, they said that a university cultured Borrelia in the lab & added it to blood.
************************************* ************************************* ************************************* LYME DISEASED BLOOD FROM THOSE THAT CHECK THEIR LYME BLOOD VIA MICROSCOPY:
***Atypical spiro to "Tennis Racket" form & then to Cyst (Discoid Gemma type) directly in HUMAN BLOOD. Tennis racket forms are widely known & seen in Borrelia obtained from ticks & wild grown in culture. https://www.youtube.com/watch?v=Eg_Id74a_4I
That's a pretty good video. Very good resolution at 1000x! Ha Ha, that's the first I've seen darkfield with a blue filter, though. The view is a little over-stimulating in my opinion.
The holes in the RBCs could definitely be a result of a Rickettsia. But, may be a result of other things, too.
Notice all the little "granules" or "crystals" that I've referred to in previous posts regarding my blood and in Dude's blood. Do you see all those granules I'm referring to in this video. Quite a few of them here. They are roughly 2-5 microns in diameter and irregularly shaped.
I think they are small colonies of spirochetes.
I wish we knew if they are on ABX or not.
I did notice what could possibly be a clump of Rickettsia bacteria. At :47 in the video it's right in the center, almost exactly 1/3 of the screen from the left. You can see individual small round objects. May be Rickettsia, but could also be lysosomes.
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