bluelyme
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Thanks tnt ..great info re swiss agent .cant wait for uos to release doc ....i got the script for dap but its so close to a sulfa i am scared stiff after bactrim episode ..the vasculitis is sorta calmed down after much iv curcumin and rocephin
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TNT
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quote:Originally posted by bluelyme: ...rifampin is kicking something as is houttynia ...
I love Houttuynia! And CSA! Both have really helped me over the past year. Not curative by themselves....but very synergistic with ABX.
Just had to put my plug in about herbals. Wonderful medicine. And Stephen Buhner is GENIUS!
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I am gonna prepare a batch of the BSK-H without the antibiotic mixture.
If anybody needs some, just message me. I might be able to help out.
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Lymedin2010
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Staphylococcus aureus, the one that causes biofilm & issues with joint replacement prosthetics & any other foreign objects in the human body (this is well known). Also the cause of skin infections & respiratory infections, including MRSA.
I would imagine this may be an added issue for Lymies & I never hear of anyone talking about this. I can see this not only being in a Lymie body, but living within Borrelia biofilm.
TNT
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Amazing!
Lymedin, you always amaze me with what you uncover! That CytoViva video of the Rickettsia is exactly what I am seeing in my live blood from the beginning! Remember the "Bartonella" organism I showed video footage of? We debated about whether they were dumbbell ketes, or a Bart-like bacteria. I finally decided it must have been overflow from leaky gut. Now I'm sure they are Rickettsias! Probably Anaplasma. Especially since I'm finding unmistakable morulas in my stains. It would be interesting to know what species of Rickettsia the CytoViva video is showing.
Here are the two videos I had posted that document my Rickettsia bacteria:
Lymedin, thanks for that video, and of the one of Borrelia. It's so good to have lab verification of carbon copies of what we are seeing under our own scopes!
dudefromkansas, welcome back! I'm sorry to hear about the scope. FWIW, there is a well-equipped Zeiss Photomicroscope III on Ebay right now for about 3 grand. I don't know what your plans are or what you may now be looking for, but it's a sweet scope:
Just gotta make sure it's right for what I need, and I am also now wary of buying used due to the last experience.
I have contemplated starting with a lower end fluorescent capable microscope.
However, I also want to enhance my culture methods first. So my work now will be focused on improving the culture method before I think about doing anything more advanced than what I am doing now.
It's time and money, so gotta make sure it is done right.
Great videos and great research provided everyone. Maybe we could get some sort of collaboration going on some of this stuff, direct collaboration.
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bluelyme
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tnt that second vid was awesome , that blo was chasing the kete with a vengance , i hope they werent colaborating but if we could just sick em on the ketes ha ha , or is phage therapy any kind of real probability with regards to risk vs benifit
posted
TNT, I want to say you are doing some good work with your stuff, and it has motivated me a bit to begin my work again.
Excellent
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TNT
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quote:Originally posted by thatdudefromkansas: TNT, I want to say you are doing some good work with your stuff, and it has motivated me a bit to begin my work again.
Excellent
Hey, thanks! I really appreciate the compliment! But, I have to say, you guys have been a great inspiration to me! It was this thread that first showed me what all was possible with microscopy. The work of Lymedin2010, S13, and PeterKemp was a huge motivation for me to get involved, especially Lymedin's. It's hard not to catch Lymedin's enthusiasm.
And, dude, your pioneering & culturing skills are really amazing! To come up with homemade BSK medium is genius! And your video capture of the kete to cyst conversion was just timeless and priceless! I think it's great we have slightly differing interests among us. It's very complimentary.
I've learned a lot, and much of it is because of you guys here! Keep up the good work fellows! And that's to all of you-- those of you who are veterans, and those of you who joined more recently.
I hope you new guys become regular contributors and this really becomes a movement.
I also wish S13 and PeterKemp would come back on and give us their great input! They both have some great microscopy skills, not to mention Lymedin2010.
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Lymedin2010
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Peter Kemp is on FaceBook & is active in the LD community. We have all hit a limitation on what we can do with a microscope, other than to see the spiros.
I have contacted Cytoviva on that video about Borrelia in blood & this is what they had to say...
"Thank you for your interest in CytoViva technology and the ability to observe Borrelia and related organisms. Our patented enhanced darkfield microscopy enables the capture very high signal-to-noise images of these types of pathogens in-situ in tissue, blood cells and other environments.
By adding hyperspectral imaging to the microscope, you can spectrally characterize these pathogens in these different environments.
The video you referenced was captured by microbiology researchers at Auburn University who were studying Borrelia. These bacteria were specifically cultured by this microbiology group.
CytoViva provides fully equipped optical microscopes for this type of imaging and can also provide integrated hyperspectral imaging as required.
Please let us know how we can support you with more insight regarding this technology.
Kind regards,"
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Any idea on the cost of those CytoViva microscopes.
I'm guessing in the range of 30,000 dollars.
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Lymedin2010
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TNT, I have seen what I called "bean' like objects in my blood too & it looks very similar to Ricketssia. I always thought they were the discoid gemma of the spiros. We do have one capture of a spiro that morphs into this structure & I would have to dig it up again. In tick juice, I do not see any discoid gemmas, but I have seen fairly large cysts & cyst small enough that they get burried & are indistinguishable from the tick gut cells.
Yea, I too would love to obtain a CSF sample. I had done 2 spinal taps before I had a microscope & have not had one since.
I am so tempted to extract some sinovial fluid from my knee at one point, as I am expecting to see more spiraling forms in that fluid.
None of the Cytoviva prices are listed, so they must be in the thousands. No need for a Cytoviva scope, as the new 4K addition the any scope will bestow it with grand improvements. Look into a good 4K camera with an adapter, such as the Canon SLR's or Sony Alpha cameras.
Check out these good fluorescent scopes you can put a lower bid amount.
TNT
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quote:Originally posted by Lymedin2010: TNT, I have seen what I called "bean' like objects in my blood too & it looks very similar to Ricketssia. I always thought they were the discoid gemma of the spiros. We do have one capture of a spiro that morphs into this structure & I would have to dig it up again.
Yes, definitely dig that one up, I would love to see it! I have seen the disc-shaped cysts of borrelia with my darkfield (like what dude showed us in the conversion video) and there is no mistaking the cysts. Darkfield seems to be the best technique for viewing them.
Also, were you able to find those pics of the other lyme patient's morulas? I assume they were on Facebook? I would enjoy seeing them, too!
quote:Originally posted by Lymedin2010: We have all hit a limitation on what we can do with a microscope, other than to see the spiros.
Yeah, I can relate to an extent. I'm just encouraging all of us to post the things we do see, even if similar things have already been posted. If nothing else, it keeps the thread alive and keeps our interests up.
I'm a little unclear if you have done any Giemsa smears, Lymedin. It's an indispensable tool for particular infections, as you already know. Also, I was wondering how you are doing these days. Since we haven't heard much out of you lately, I figured you were still pretty low. That's been my impression. Maybe you are doing well enough you are busier with life. What are you doing for treatment now? Have you considered BVT??? I am making some real progress on it, along with herbs and an ABX or two. The BVT didn't really start kicking butt until I got to the nominal dose, which is 10 stings per time. But, it took time to build up to that. Of course, maybe you've already considered it since you follow Eva Sapi's work very closely.
And, S13, if you are still out there, pop in and give us an update and show us anything of interest! Anything.
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TNT
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quote:Originally posted by Lymedin2010: TNT, I have seen what I called "bean' like objects in my blood too & it looks very similar to Ricketssia. I always thought they were the discoid gemma of the spiros. We do have one capture of a spiro that morphs into this structure & I would have to dig it up again.
quote:Originally posted by TNT: I have seen the disc-shaped cysts of borrelia with my darkfield (like what dude showed us in the conversion video) and there is no mistaking the cysts. Darkfield seems to be the best technique for viewing them.
In the same darkfield sample as the cysts, I also clearly saw what you are calling "bean-shaped" organisms. So, I am pretty sure what I am seeing are not the same things. I'll go back and look at that and maybe put it up.
[ 12-13-2016, 02:00 PM: Message edited by: TNT ]
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TNT
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Lida Mattman's 4th edition of Cell Wall Deficient Forms- Stealth Pathogens is finally being released and you can order your copy now!
It is on a pre-order sale at Amazon for $149.95!
I just thought I would let you guys know!
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Lymedin, Staph. Aureus is an extremely common bacteria that can cause significant infections. Usually a dermatological infection, anytime someone says they got staph, that's it. Cuts, abrasions, etc, that get infected are generally always caused by staph aureus. It's a common infection in surgeries, and is always treated prophylactically for that.
It's also a concern in our microscopy, especially live microscopy, because it is one of the most common contaminants. It can render cultures useless. If you touch something ungloved, you might contaminate it.
If you want a break from staining for Borrelia, take some swabs of your skin. Practice and refine staining that. You'll get staph. aureus on a skin swab. You can even just take a sterile q tip and dab a bit of saline on it, swab your skin, and then transfer that to a slide. It's a good way to practice. And you can get practice differentiating between the different types of bacteria and determine gram negative or gram positive, etc.
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Lymedin2010
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TNT, thanks for asking. I've been trapped in hell, as we all have, with many pains, exhaustion & my brain is on fire & hence less posts on here.
We have all become more experienced here & it requires a bit more vigor & brain power. Every now & then I got a slight relief from the brain fog. ABX & even herbs seem to have just stopped working.
I had done Giemsa in 2012 & I bought a cheaper batch and the stains were filled with tons of artifacts. I have just purchased the same strain from your recommendations & could probably use a lesson in the finer details & any tricks of the trade that you might have happened to stumble upon.
Dude, yea I took a Courseca course on Aureus & it was very interesting, as I did not know these things before the course. Ever since that course I have been upon to tertiary infections having a profound impact on Lyme as a whole & probably from multiple pathogens more likely.
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Lymedin2010
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When we asked Alan MacDonald about the My Horrific Lyme Video in an email, this is what he responded within that email & he then posted his response publicly on LymenetEurope. You can check the post for yourself.
"Dear Zoran,
I have viewed your video and I concur that your images do indeed demonstrate Spirochetal forms
in various profiles. I would avoid the word "debris" because I have evidence from my DNA Probe studies
with Probes absolutely specific for Borrelia DNA ( two sites on The Chromosome- Burgdorferi and two sites on the
chromosome of Borrelia Miyamotoi) that each and every DNA containing membrane bound Borrelia
variant ( spirals, cylindricals, Segmented, String of Pearls, Granular forms, and Liposome (Blebs) , and Cystic forms
are ALL CAPABLE of regenerating the spiral Borrelia form under ideal circumstances. You have described
"medusa" profiles of Borrelia, and this is a good name for what you see in some of your images.
You have not described the Biofilm Borrelia communities. These are extremely important, and my work has proven with
DNA Probes for Borrelia DNA ( Molecular Beacons) the Biofilm communities of Borrelia
dwell Inside of Amyloid Plaques in Alzheimer's disease. I attach some images for you to inspect from DNA Probe hybridization
TNT
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quote:Originally posted by Lymedin2010: TNT, thanks for asking. I've been trapped in hell, as we all have, with many pains, exhaustion & my brain is on fire & hence less posts on here.
We have all become more experienced here & it requires a bit more vigor & brain power. Every now & then I got a slight relief from the brain fog. ABX & even herbs seem to have just stopped working.
I'm so sorry to hear this, although I have been suspecting it. I have been having success with BVT and I don't think you should try it.... I KNOW you should try it!! It's better than CBD! REALLY! I forget where I put my prescription pad, otherwise I would write you a script for it!
quote:Originally posted by Lymedin2010: I had done Giemsa in 2012 & I bought a cheaper batch and the stains were filled with tons of artifacts. I have just purchased the same strain from your recommendations & could probably use a lesson in the finer details & any tricks of the trade that you might have happened to stumble upon.
I think the Romanowsky stain will give you a clue as to what is going on. Unless it simply is Borrelia out of control, or something like a virus.
Did you get the "Blue" or the "Red" stain from that company? Either way, they are very similar and should be able to see the same things. The red is equivalent to straight Wright's stain, and the blue is equivalent to Wright-Giemsa. Both are Romanowsky, and either one will be very helpful.
There is no mixing with those products, so there really are no steps other than having a dried smear and staining it. After preparing my slide I allow the blood to dry by placing it in my oven at 100 degress F. That temp is used when doing a smear for Malaria so that the parasites don't destruct in the process of drying. I do it that way too in case it's the same for other apicomplexans.
I draw out of my bottle of stain very carefully with a sterile pipette. I have found that you get artifacts if you don't sufficiently flood the top of the slide. That said, you still only draw out maybe a 1/2 an ml, or less. So, only a very small amount is used. But, if you don't use enough stain, it will begin to dry on the slide before you rinse, and that produces the artifacts. After allowing the stain on the slide for 2-3 minutes, I drop the slide in a coplin jar of distilled water and allow to soak for about 1-2 minutes before rinsing with distilled water from one of those lab squirt bottles. Allow to dry in a covered container (to minimize airborne contamination), and you are ready to view.
I plan on posting some pics and video from my darkfield demonstrating the difference between cysts and Rickettsia-like bacteria. Stay tuned.
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Lymedin2010
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There is a special PCR DNA sequencing going on now at uBiome.
Sequence all 5 locals from: gut, nose, skin, mouth, & genitals for less than $100, rather than the $400 usual price.
I added my blood to the nose sample, as sometimes there is blood in the nose anyways. Many times there is blood in my nose, so a little more won't hurt.
Lymedin2010
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I bought the blue one. Thanks for the instructions & it sounds easy enough, but it always does on paper & a bit more messier & tricky in person until you have done it a few times.
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I had sent off some blood slides to Dr. MacDonald to have stained, but then he stated he had retired and, as such, was unable to continue that work. So he was not able to get around to staining my slides. It was for MS research and was provided free of charge.
If he is active again, and doing that work....drop him my name and let him know I still have MS, haha. I'm not gonna bother him again. It'd be nice to have a definitive answer, though. I could even get him a CSF sample. I can provide you details in a message.
Also, you can find online courses to enroll in sometimes relevant to microbiology, etc. www.edx.org is a good place. They have courses taught by professors from MIT, Harvard, etc, free to take.
Anyways, I made a short video. Slow internet here in Kansas, like the life here, haha. Its a video of a gemma with a spirochetal body (tennis racket) showing the development of a second body, perhaps something like transverse fission occurring (one bacteria becoming two, asexual), perhaps simply a single organism reverting to an atypical spiral form, or perhaps it is a small cyst with a second spirochete emerging (though it is rather flat and discoid, making me think it's either a single organism, or the transverse fission).
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Lymedin2010
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Dude, I would love to see the video. Thanks for the edu link.
I will have to find the gemma conversion video again, may take some time.
Check out this spiro a fellow Lymie captured, as it moves & looks exactly like what I see in the ticks that I observed, at least for some of the spiros. Some are longer & some are shorter too & there is no standard size it seems.
This is the same exact form & movement we see in the CytoViva video & what I see in my blood from time to time. Granted there will also be false-spirochetes in our blood that can many times come pretty damn close to this, but will not have the complete vitality & movement of a true spiro.
quote:Originally posted by thatdudefromkansas: https://youtu.be/YMdunXbr2eI
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TNT
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You guys are referring to "gemmas." How are gemmas different than cysts?
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Lymedin2010
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I think Gemmas are immediate cysts & cysts can grow larger & can in itself have various morphologies from perfectly round, to oval, to wavey, and at times with a spiro protruding out.
On another note, Do you want to check ticks under a microscope & then want to confirm presence of Bb?
"
CARE PLUS 38404 LYME BORRELOISE TICK TEST
Quickly and accurately indicates presence of the Borrelia bacteria in the tick, which may cause Lyme disease. No waiting time for laboratory results. 95,8% reliability within 10 minutes. Includes checklist. Provides important information for your doctor, as a result of which any necessary treatment can be applied quickly – this increases the likelihood of success.
You must act quickly after being bitten by a tick. We know earlier is certainly better when it comes to the removal of the tick, detection and treatment of Lyme disease. Use the Care Plus® Tick Test to immediately determine whether the engorged tick is carrying Borrelia bacteria, which can cause Lyme disease. This (self-)diagnosis test looks like a pregnancy test and provides a 95,8% reliable result within 10 minutes. (study 2011)
This self-administered test and the checklist help provide information early on to you and to your healthcare practitioner. In the event of a positive result, and when you experience any symptoms, we recommend to consult your doctor and take the filled in checklist with you. On the checklist you note valuable information about the bite (date, site on your body, area), the tick itself (size), skin reddening, test result Tick-Test and various possible symptoms. While it’s not a substitute for clinical advice, this test in combination with the checklist can help motivate people, who otherwise might have waited, to seek medical attention, especially if they have a positive result for bacteria in the tick. Contents: Tube, liquid, wooden stick, pipette, test cassette, leaflet/checklist.
User instructions: Step 1. Remove the tick. Step 2. Place the tick in the empty tube. Step 3. Crush the tick on the bottom of the tube with the wooden stick. Step 4. Use the pipette to add 10 drops of liquid to the tube. Step 5. Close the tube and shake it vigorously. Step 6. Use the pipette to suck some liquid from the tube.Note: do not transfer any parts of the tick itself, as these can block the test field. Step 7. Put 5 drops of sample solution into the funnel shaped opening of the Care Plus® test cassette and lay down the test cassette in horizontal position. Step 8. The test result will be visible within 10 minutes.
Negative test result
A line appears in the control area (C). This means that the test has been carried out correctly. If no line appears in the control area (C), the test result is invalid and the test procedure must be repeated using a new test. No line appears in the test area (T). This means that no Borrelia bacteria have been found in the tick.
Positive test result
A line appears in the control area (C). This means that the test has been carried out correctly. If no line appears in the control area (C), the test result is invalid and the test procedure must be repeated using a new test. A line appears in the test area (T). This means that Borrelia bacteria have been found in the tick. In the event of a positive result, we recommend to consult your doctor and take the filled in checklist with you.
Take note: the intensity of the lines may vary, but this does not change the test result in any way.
Tip: Even if the Tick Test indicates a negative result, always visit your doctor if a red ring (Erythema migrans) develops around the bite area or if you experience any symptoms."
TNT
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So, the gemmas are the most juvenile stage of the cyst? Ok. I just wanted to make sure I had my morphology correct because of these videos I just uploaded. About the time you think you have all the morphologies of borrelia figured out, BAM, you're hit with another one! I can't wait til Lida Mattman's 4th edition arrives!!
Lymedin, I've seen that test kit advertised, and wondered how accurate it really was. But, not because I'm going to be collecting and messing with any ticks, that's for sure!
TNT
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I have a video with ketes, gemma cysts, and Rickettsia bacteria all in the same frame but I have to edit the sound on that one. I will post it as soon as I'm able to.
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TNT
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Have a few interesting darkfield pics of gemma cysts inside some leukocytes. Will post them later as well.
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TNT, what is your microscope setup?
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TNT
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It's an American Optical 10 series with an oil darkfield condenser. Those darkfield videos I just posted are with a funnel stop in the 100x objective, so my darkfield may not be as precise as it could be on those videos. I recently got a 100x plan objective with an iris, but have not done any darkfield with it yet. Also, those videos were with a scope that currently has a left hand stage control, which made it difficult for me to use, thus the shakiness in the video.
See the pics of my setup a couple pages back.
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check out from about 45 seconds onward.
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TNT
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Dude! Those are those granules I was talking about! The smaller ones look identical to the ones I've seen in my blood. It looks like they (the larger ones) are some type of pregnant gemma.
Are you off ABX right now by any chance?
Great video!
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And I have noticed a significant increase in the presence of these bodies as well as the spirochetal ones upon stopping, along with increased symptoms.
Had to take a break due to GI stuff from the (I presume) clarithromycin.
Interesting, I saw a large number of these granular bodies. It looked more like a cluster of spirochetal bodies attached to each other or something else.
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I have also purchased a PlanApo 100x oil objective with iris to use.
Hopefully it will bring some resolution to the aberration you see with y current objective, and will also provide me with higher resolution so this stuff is a little clearer.
I'm also messing with the digital zoom on my camera at 400x to see if i can get increased resolution at around 2,000x.
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Currently watching and recording a spirochete emerging from a cyst. Will have that video up later. Batter on camera is getting low, but hopefully I can record long enough to get the spirochete separating.
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TNT
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How'd it go, dude, did your camera battery hold out?
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Waiting on my slow internet to finish upload.
Nope, didn't hold out. Would have taken quite a while, and the file would have been massive anyways. That would have taken days to upload with my current internet speed.
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It's Plan Apo, as opposed to Plan Achro, and I see a big difference.
I think it was well worth the price. Minute details of the bacteria are more readily visible, as well as surface detail of granules.
More use will give a better understanding of all the visual advantages. I have a test video I will share whenever it has uploaded.
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TNT
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quote:Originally posted by mustardseed2: I started doing some staining. This showed up fairly bright. Any idea what this could be? OR just an artifact from a poor stain?
I seems to have a lot of random stuff like this kicking around:
I agree with Lymedin, I see no particular morphologies in those pics. As a tip: When you put stain on top of your dried smear, make sure you sufficiently flood the top of the slide. That way it will not begin to dry before you rinse. This will minimize the possibility of artifacts.
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